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We narrowed to 39 results for: SEC

Showing: 1 - 20 of 39 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...Depositor Collections INTRSECT Plasmid Collection INTRSECT Plasmid Collection INTRSECT Implementation Plasmids...SOM-IRES-Flp). Image from Fenno et al., 2014. INTRSECT INTRSECT (intronic recombinase sites enabling combinatorial...Find intersectional expression plasmids that combine multiplexed recombinases, including Cre, Flp, and...neurons defined by a single gene or projection). Intersectional expression approaches combine multiplexed recombinases... multiple parameters. Figure 1: Examples of intersectional cell population definitions using recombinases...recombinases. A) Cre and Flp are multiplexed to enable intersectional viral targeting of populations expressing only...recognition sequences within synthetic introns. INTRSECT was first shown as a proof-of-concept targeting...

  2. Worm Expression Resources

    Type
    Collection
    ... links to protocols, can be found below: SapTrap-SEC kit for C. elegans genome engineering - Bob Goldstein...incorporate a self-excising drug selection cassette (SEC). More information regarding this kit can be found...

  3. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...parameters: 95 °C for 15 min, 35 cycles of (95 °C for 30 sec, 60 °C for 1 min, 72 °C for 1 min), and 72 °C for...Along with the video, you can find the protocol section from the original publication below. You can also...construct. Electroporate cells with 250 volts for 5 msec in a 2 mm cuvette using an electroporation system...

  4. Antibody Guide

    Type
    Collection
    ...similar factors. Secondary antibodies Secondary antibodies, often called secondaries, are usually much...and a conjugated secondary antibody specific to your primary antibody. Multiple secondary antibodies will...if your fluorophore is bound to a secondary antibody, the secondary antibodies must be able to distinguish...Therefore, secondary antibodies are species specific and are often referred to by [secondary source] [primary...[primary source secondary recognizes], e.g. , “goat anti-rabbit.” Choose a secondary that recognizes your...applications, and is covered in the following Isotypes section. The variable region of the antibody is the antigen...the amplification of signals by having multiple secondary antibodies binding to a primary antibody which...

  5. Immunology Research Plasmids and Resources

    Type
    Collection
    ... the secretion of antibodies, and also function as antigen presenting cells. B cells in secondary lymphoid...chromogranin A (parathyroid secretory protein 1) CGA CHGB chromogranin B (secretogranin 1) SCG1 CKLF chemokine-like... SDS, SWDS SCG2 secretogranin II (chromogranin C) CHGC, SN, SgII SCGB3A1 secretoglobin, family 3A, member...HIN1, LU105, MGC87867, PnSP-2, UGRP2 SCT secretin - SCTR secretin receptor SR SCYE1 small inducible cytokine...SDCN, SYND3 SDC4 syndecan 4 MGC22217, SYND4 SECTM1 secreted and transmembrane 1 K12 SEMA3A sema domain,... a recurrent infection and rapidly initiate a secondary immune response. Browse Plasmids for Immunology...

  6. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...Return to top Secretory Pathway The secretory pathway is named for its role in secreting proteins from...Voeltz 49155 mCh-Sec61 beta Endoplasmic Reticulum Sec61 mCherry Gia Voeltz 49154 BFP-Sec61 beta Endoplasmic...Posts Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization... shipped. Many of the proteins that enter the secretory pathway require chaperones to help with folding... or search the table below to find markers of secretory pathway components. ID Plasmid Structure Targeting...Endoplasmic Reticulum Sec61 BFP Gia Voeltz 73209 pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL Endoplasmic Reticulum...

  7. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ... for Imaging Insulin Secretion A Ratiometric Sensor for Imaging Insulin Secretion in Single beta Cells...S0896-6273(20)30398-6. Edward Boyden Calcium Intersectional viral expression of GCaMP6m/s in cells depdent...Vcre Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional... pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Expression of GCaMP6f in PV...755-60. Loren Looger , Eric Schreiter Calcium Intersectional viral expression of sRGECO (constitutive or...Flp-dependent) Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of Diverse Functional... pii: S0896-6273(20)30432-3. Karl Deisseroth , INTRSECT 2.0 Project Calcium Mitochondrial and cytosolic...

  8. Luciferase Plasmid Collection

    Type
    Collection
    ...organisms, ranging from bacteria to aquatic animals to insects. Luciferase catalyzes the oxidation of a substrate...Rluc), and NanoLuc® Luciferase (Nluc). Luc2 is the second-generation version of Firefly luciferase that ...transcriptional reporters. However, they are not secreted, so cells must be lysed or the substrate must ...to measure luminescence. Gaussia luciferase is secreted and more stable, making time course experiments...size. NanoLuc® Luciferase is small, bright, and a secreted version of the protein is available – these attributes...applications. Browse the Collection Highlights section to learn more. Highlights of Luciferase Tools in...ATP Timothy Ryan 24348 pAC-hRluc Renilla Actin5C Insect expression of renilla luciferase Liqun Luo 51970...

  9. Antibody Plasmid Collection

    Type
    Collection
    ...in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits include consistency between lots...mammalian tissue culture cells, the R-mAbs are secreted into the culture media and can be collected for...domains to obtain recombinant hybridomas, which secrete antibodies as Fab' fragments, isotype-switched ...antibodies against zebrafish neural receptors and secreted proteins suitable for wholemount immunostaining...mCherry Monoclonals, Nanobodies, Sybodies, or ScFVs Secondary antibodies (search for "anti-mouse" or "anti-rabbit...

  10. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...express a gene; however, we have included a function section for if you want to stably express an insert using...vector pFA6a vectors - Epitope tagging in S. pombe Insect/Baculovirus UAS, MT, Polyhedrin Targeted Gene ...LIC - Vectors from the Gradia lab pFB-LIC-Bse - Insect expression vector from Gileadi lab Worm unc-54,...bacterial expression (Gateway) pYD1 - Expression, secretion, and display of proteins on the extracellular ...pFastBac LIC vectors and pFastBac Dual LIC vectors for insect expression Fluorescent proteins (GFP, mCherry, ...

  11. Antibody Production

    Type
    Collection
    ... and a species-specific fluorescently-labeled secondary antibody. The fluorescence pattern is compared...untransfected control. Immunohistochemistry (IHC) Tissue sections are fixed, permeabilized, and labeled with the...species-specific horseradish peroxidase (HRP)-conjugated secondary antibody. The tissue labeling pattern is compared...antibody and a species-specific HRP-conjugated secondary antibody. Protein expression is compared to an...

  12. AAVED

    Type
    Collection
    ...achieve targeted neuronal manipulation (aka intersectional control). The end result of the meeting will...protocols showing examples of AAV strategies for intersectional control. Please see our list of suggested materials...representing a reference for AAV use in neuronal intersectional control. Through your participation in this...open science! Please see the meeting preparation section for how to best help contribute to this publication...

  13. Arf GTPase Family

    Type
    Collection
    ..., Gateway GEF Iqsec1 9922 1114 Gateway GEF Iqsec2 23096 1488 Bacterial (pET) GEF Iqsec3 440073 1182 GEF...

  14. Structural Genomics Consortium Plasmids

    Type
    Collection
    ...AmpR 26095 pFHMSP-LIC-N N/A Expression vector for secreted proteins with N-terminal hexahistidine tag and...AmpR 26100 pFHMSP-LIC-C N/A Expression vector for secreted proteins with C-terminal hexahistidine tag and...

  15. All Antibodies

    Type
    Collection
    ...experimental details in the Antibody Applications section of our product pages. We also include negative ...affinity reagents such as scFvs, DARPins, Fabs, and secondary antibodies. To learn more about antibodies and...

  16. Institute for Protein Innovation

    Type
    Collection
    ...antibodies targeting families of cell surface and secreted proteins from a collaboration between Addgene ...antibodies targeting families of cell surface and secreted proteins (glypicans and integrins) and common ...

  17. Zinc Finger Consortium: OPEN Reagents

    Type
    Collection
    ...future publications. For your Materials and Methods section: "The plasmid kit used in this publication was ...(Addgene kit # 1000000013)" For your Reference section: Oligomerized pool engineering (OPEN): an 'open-source...

  18. CRISPR Plasmids - Drosophila

    Type
    Collection
    ... been designed for use in Drosophila and other insects.... been designed for use in Drosophila and other insects. Cut Fully functional CRISPR/Cas enzymes will introduce...

  20. Cre-lox system

    Type
    Collection
    ...recombinases that are less toxic in Drosophila hsp70 Insect Rubin 32606 Ttr:Cre Cre expressed in visceral endoderm...EGFP, and sgRNA expression hSyn AAV Zhang 60820 pSECC Cre, Cas9 and sgRNA expression EF-1 alpha Lentiviral...dSTOP-loxP>-myr::GFP Cre dependent myr-GFP expression Insect Rubin 51503 AAV pCAG-FLEX-tdTomato-WPRE Cre dependent...
Showing: 1 - 20 of 39 results