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  1. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    ...the work you've done. If you want to maximize the impact of your communication, you will also have to explain...communicate with non-scientists, to establish broader impacts for their work in education and policy and to draw...

  2. A Novice's Perspective on Neuroscience - SfN2017

    Type
    Blog Post
    ... about and develop technologies that will have impacts on many different facets of life. It also seems...neuroscience. AAV tools from Addgene are already having an impact on the field Not to toot our own horn too much,...

  3. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...offices, Addgene would not be able to have such an impact on scientific materials sharing. I would also like...further fulfilling our mission for an even bigger impact. In early 2016 we surveyed hundreds of scientists...

  6. Clear and Accessible Writing

    Type
    Blog Post
    ...the use of alt text to describe images in online spaces. Alt text is used by screen readers to provide ...professional development workshop, “Make a Greater Impact Using Clear and Accessible Scientific Writing.”...

  7. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...a gRNA is useless without an adjacent PAM or protospacer-flanking sequence (PFS) in some cases. The resource...the protein would be helpful to know for viral packaging limitations. Maybe you want to read literature...

  8. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ...with some of our most popular biosensors already packaged and ready to deliver. Ready to give biosensors...AAV as your vehicle, we’ve got you covered with packaging plasmids! References and Resources References...

  9. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...tricky to find: each of the factors can negatively impact both translation and fidelity of translation, leading.... Or, to quote Ryan Mehl, PhD, GCE has “10x the impact of PCR. But it’s 100x more complicated.” And Ryan...

  10. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ... these amazing tools in your experiments? Let’s unpack the myriad number of emerging viral applications...just one at a time. Many of these libraries are packaged and delivered with viral vectors instead of a ...

  11. How Addgene Creates Content

    Type
    Blog Post
    ...over their favorite science topics. What better space to do that than in the Addgene blog?  Fig...expertise of our antibody team to rely on. High-impact content like the antibody guide always gets an ...

  12. Antibodies 101: Monoclonal Antibodies

    Type
    Blog Post
    ...of the antibody - the paratopes - may change and impact the specificity and avidity of the antibodies produced... chain or light chain genes within a hybridoma impacts the specificity, avidity, and number of different...

  13. AAV Q&A with Tim Miles

    Type
    Blog Post
    ...why hot dogs are sold in packages of 8 yet hot dog buns are sold in packages of 10…)  If you missed the...

  14. Hot Plasmids - February 2022

    Type
    Blog Post
    ... of performing similarly to Cas9 screens in a compacted library. Overall, Cas12a’s ability to target multiple...significant off-target effects, yet small enough to be packaged in AAV. A dCasMini-mediated adenine base editor...

  16. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...editing, but it retains its gRNA-directed targeting capacity. By using a gRNA to target fluorescently labeled...that they could evaluate the degree of chromatin compaction even for this small distance! To the authors’...

  19. Summer SciComm: Preprints

    Type
    Blog Post
    ...scientists know the results, your work won’t have any impact! There are lots of ways scientists communicate...efforts, inspire future research, and increase their impact. Open Access and Transparency Another major benefit...

  20. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...amazing affinity reagents we just didn't have the space to give proper credit to. Thankfully, as Addgene's..., or be able to access an epitope folded into a space that would size-exclude an antibody. In imaging ...

  21. Addgene: The First Twenty Years

    Type
    Blog Post
    ...distributed milestone! We were thrilled to see the impact our repository was having on the scientific community...expanding our viral vectors service to include AAV Packaged on Request! “I love Addgene. I think resources...

  22. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    ...that there are sex differences in the therapeutic capacity of stem cells. Yet, very few researchers consider...Future of Research Learn How Notions of Scientific Impact Are Evoloving Check Out Extracurricular Activities...

  23. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...the many freely-available DNA-editing software packages to assemble your expected full plasmid sequences...when designing a sequencing primer, you need some space between your primer binding site and the first feature...

  24. Engaging with science and society at pgEd

    Type
    Blog Post
    ...But as the science moves forward at a blistering pace, it is becoming ever more urgent for scientists ...at pgEd, I take on a variety of projects in the space of science education, communication, and policy....

  25. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...and selection markers. Need fewer components? A spacer can be inserted instead, allowing everything to...entirely, but instead will have to replace it with a spacer.) Each component available on the Fragmid website...

  26. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...FRET pair. If you need to free up crowded optical space for multiplexing, GECO sensors are available in ...the calcium sensors described are also already packaged into viral vectors and ready to go. Pro tip: Vectors...

  27. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...allows you to study signaling pathways and the impacts of neural circuits on behavior. Figure 1:...channels. When working with PSAM receptors, your impact on the cell is determined by what IPD your PSAM...

  28. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...Gag structural protein on yet a third plasmid (packaging plasmid). Viral production then proceeds by transfection....   Figure 1: Separation of retroviral packaging components onto distinct plasmids for safety.  ...

  29. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...depiction of chromatin within a nucleus -> DNA compaction around histones -> Examples of histone-modifying...flanked by randomized nucleotides in place of a protospacer adjacent motif (PAM). They're used to determine...
Showing: 271 - 300 of 556 results