We narrowed to 642 results for: ato
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TypeBlog Post...delivery of Cas9 endonuclease, Cas9 nickase, Cas9 activators, Cas9 repressors, select gRNAs, and CRISPR pooled...expression of a gene under the control of the tet operator. AAV AAVs are the workhorses of both chemo- and...
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Quantifying DNA? Here are Five DNA Quantification Methods to Consider
TypeBlog Post... at 230 nm. The A260/A280 ratio is used as an indicator of DNA purity. Ideally, this number should be ...like a PCR product). This method also gives an indicator of DNA or RNA contamination based on the presence... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog Post...following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...impact on virus replication. While we and our collaborators are starting these experiments, there's so much... -
Celebrate National Book Month with Addgene
TypeBlog Post...relevant science." - Randall Carter, Contracts Administrator Inferior: How Science Got Women Wrong - and ... Tsang, Science Communications and Marketing Coordinator Non-science favorites The Mistborn series by... -
New and Upcoming Viral Vectors - June 2019
TypeBlog Post...37825 AAV1 pAAV-CAG-GFP 59462 AAV2 pAAV-CAG-tdTomato (codon diversified) 114472 AAV5, AAV8 pAAV-...ppAAV-CKIIa-stGtACR1-FusionRed New tetracycline transactivators Plasmid Serotype Name 99120 AAV1 pAAV-ihSyn1... -
Fluorescent Protein Travel Awards - Protein Variants, a Serotonin Sensor, and an Artificial Leaf Replica System
TypeBlog Post...serotonin is a well-known but poorly studied neuromodulator, and evidence suggests that it is incredibly...sensor). In preliminary studies with the lab’s collaborators, they were able to use the serotonin sensor ... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...For my undergraduate degree, I majored in human anatomy, but I also took classes in biochemistry, microbiology...the time I was ready to graduate with my BSc in Anatomy, the University of Otago had established the Centre... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...SF-Venus-iGluSnFR). iGluSnFR3: Improved Glutamate Indicators for Synaptic Imaging However, the first two generations... 30377363 Aggarwal, Abhi, et al. . “Glutamate indicators with improved activation kinetics and localiation... -
Science Career Options
TypeBlog Post...skills include: Science Reporter Scientific Illustrator Research and Development Scientific Advisor..., including: Livestock Breeder Scientific Illustrator Plant Breeder Zoological Worker Conservationist... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...bodies do for you? R bodies are robust linear actuators that could be used in a variety of applications...applications, purified R bodies could also perform as actuators in microelectromechanical systems (MEMS) devices... -
Resume Writing for Non-academic Science Careers
TypeBlog Post...how I got my current job as a Communications Coordinator. Wondering how you can develop more transferable...Theresa Liao (@theresaliao) is the Communications Coordinator for the Department of Physics & Astronomy, University... -
Advice for Scientists Starting a Lab
TypeBlog Post...will choose to start their own academic research laboratory. How does one even go about starting a lab? Obviously... does 7 or 8 jobs: “a teacher, departmental administrator, lab and safety manager, researcher, adviser... -
New and Upcoming Viral Vectors - May 2020
TypeBlog Post...AAVrg pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls... -
Hot Plasmids - February 2022
TypeBlog Post... cassette provides increased simplicity for combinatorial genetic screens, and this optimized toolkit ...by the fusion of Cas12f with a transcriptional activator, CasMINI is able to induce high levels of gene... -
Luminescent Imaging with Nano-lanterns
TypeBlog Post...Since their first publication in 2012, the Nagai laboratory has assembled a collection of multicolored nano-lanterns... Nano-lanterns and your experiments The Nagai laboratory has a done a wonderful job characterizing Nano-lanterns... -
Viral Vectors 101: Viral Vector Elements
TypeBlog Post...respectively, act as RNA polymerase II promoter and terminator regions and are slightly different in sequence.... Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press; 1997. Principles of Retroviral Vector ... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog Post... Expression Low Tropism for Mouse Cells and Hematopoietic Cells Low Packaging Capacity (~4.5kb From...S, Varmus H. Retroviruses. Cold Spring Harbor Laboratory Press; Cold Spring Harbor, NY, USA: 1997. PubMed... -
How Addgene Creates Content
TypeBlog Post...technical skills, and experiences from thousands of laboratories that makes up our Help Center, blog, website...graphic designer upon request that the science communicator "just sketch a western blot out quickly." Final... -
7 Tips to Secure a STEAM Internship This Summer
TypeBlog Post...don’t guess. Simply reach out to the program coordinator with your question(s). This precautionary step...notice within 24 hrs, send a note to the program coordinator. 6. Virtual interview prep Prepare for your... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...Vellai lab’s new plasmid, T444T, contains T7 terminators adjacent to the T7 promoters to prevent the incorporation...can take as long as several days. In 2015, the laboratory of long-time Addgene depositor Alice Ting used... -
CRISPR 101: Non-Homologous End Joining
TypeBlog Post... sequence (“microhomologies”). NHEJ doesn’t obligatorily introduce indels. Given the end structure of...JS, Konermann S, Trevino AE, Scott DA, Inoue A, Matoba S, Zhang Y, Zhang F (2013) Double Nicking by RNA-Guided... -
3 Challenges in Plant Synthetic Biology
TypeBlog Post..., one that is not food, not medicine, and not mandatory – people can decide for themselves if they want...duty to pursue a degree in a synthetic biology laboratory and received her M.S. from CSU in 2014. Outside... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog Post... probably left your plate for too long in the incubator. The small colonies are called satellite colonies...your sequencing results back, you can use the chromatogram to spot whether any discrepancies between your... -
Transferable Skills Guide: Public Speaking
TypeBlog Post...interview better! As a scientist turned science communicator, I’ve done a lot of public speaking, and I'm... turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving board” so you end... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog Post... plasma membrane to be activated by the DREADD actuator after it has been administered. Although we found...Research Center. Using a combination of in vivo and anatomical techniques, her research aims to gain a deeper... -
The Future of Research Symposium Boston 2015
TypeBlog Post...compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains contested...counselor, Director of Education for the Jackson Laboratory for Genomic Medicine and a Senior Research Associate... -
Rabies and Neuronal Tracing
TypeBlog Post...this study laid the foundation for how different anatomical regions of the brain receive inputs and compute.... 5. Nassi JJ, Cepko, CL, Born RT, Beier KT. Neuroanatomy goes viral! Front Neuroanat. 2015 Jul 1;9:80... -
Educational Planarian Tools for Teaching Developmental Biology Techniques
TypeBlog Post...experiments in the beginning. In most classrooms, the laboratory experience is focused on classical embryology...for other developmental models including flies, nematodes, and zebrafish Find plasmids for Mammalian RNAi... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...experiences to undergraduate students (1, 2), but educators face significant challenges in designing suitable...Functional genomics research in an introductory laboratory course. CBE-Life Sci. Educ. 17, ar1. (2018). ... -
Open Resources and Plasmid Tools For Studying C. elegans
TypeBlog Post...open worm browser, which allows you to view the anatomy of worm layer by layer, the NeuroML C. elegans ...neural network, and Sibernetic, a fluid mechanics simulator that can be used to simulate muscle tissue as ...