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  1. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...Site-Specific Genome Alterations. Edited by Doudna J and Sontheimer EJ. Methods in Enzymology Vol. 546; (2015). PubMed...inactive CRISPR-Cas9 using asymmetric donor DNA. Richardson CD, et al. Nat Biotech. 34(3); (2016). PubMed...

  3. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...do aptamers! Corn was recently described in 2017 (Song et al., 2017). Corn naturally dimerizes, and this...PMID: 28747322. PubMed Central PMCID: PMC5602116. Song, Wenjiao, et al. "Imaging RNA polymerase III transcription...

  4. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    ...’s software in order to generate signals in a reasonable range. If colleagues in your group already have...interest can still be gated out later. Based on personal preference, some people like to make stricter ...

  6. Harnessing Bacterial Toxins for Allelic Exchange

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    Blog Post
    ...arguably the most widely and productively used  (Thomason et al., 2014). In recent years, CRISPR-Cas9 technology...reports 5 (2015): 15096. PubMed PMID: 28060411. Thomason, Lynn C., et al. "Recombineering: genetic engineering...

  7. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...is also being realized. There are a variety of reasons to consider using genome editing to change the ...yellow striate mosaic virus (Gao et al., 2019) or sonchus yellow net rhabdovirus (Ma et al., 2020) have already...

  8. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...RNAs." PloS one 11.2 (2016): e0149483. Rogers, Jameson K., and George M. Church. "Genetically encoded ...-tagged human ORF clones to allow testing and comparison of different cell-free expression systems; the...

  9. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post
    ...structure associated to these tools, skills, and personnel Define pricing according to all of the needed ...managers, and techs’ financial books.  To find personnel salary ranges, go to online job information sites...

  11. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    ...fluor through a process known as bioluminescent resonance energy transfer (BRET). The fluor then emits light...calcium release have been made using fluorescent resonance energy transfer (FRET) probes. These probes generally...

  12. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    ... J. H., Kim, Y. S., Machado, T. A., Quirin, S., Benson, B., Kadmon, J., Raja, C., Chibukhchyan, A., Ramakrishnan... Jason Nasse...

  13. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...Sleeper, M. M., Sanmiguel, J., Wu, D., Gao, G., Wilson, J. M., & Sweeney, H. L. (2008). Adeno-Associated..., K. L., Ayasoufi, K., & Barry, M. A. (2019). Comparison of Gene Delivery to the Kidney by Adenovirus,...

  15. Cancer and the Immune System: Deciphering the Relationship

    Type
    Blog Post
    ...surface targets by tumor cells, are a few of the reasons antibodies may result in a lack of response. Antibodies...efficient immunotherapy must be to reach a flexible personalized treatment, and not universal correction of cancer...

  16. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...J. M., Xu, A., Sisley, T. A., Weissman, J. S., Adamson, B., & Liu, D. R. (2021). Efficient C•G-to-G•C ...s41587-021-00938-z Koblan, L. W., Doman, J. L., Wilson, C., Levy, J. M., Tay, T., Newby, G. A., Maianti...

  50. Countdown to Halloween @Addgene

    Type
    Blog Post
    ...plasmid? Our lab paid tribute to the changing seasons – and they also invited Father Time to the party...
Showing: 451 - 500 of 721 results