...preferences to help you identify proximal lines you care about. If you have cell lines without a certified...

...pleasant, it’s far worse for employees if you don’t care enough to tell them about areas they need to improve...

...Monday's talks alone were worth the trip. Dr. Dana Carroll's excellent keynote address was the first of 19 ...

... Caroline LaManna...

...Director Joanne Kamens several years ago through the Healthcare Businesswomen’s Association and it was through...

...detection methods did you use for expression of AAV cargo? Did you use GCaMP expression? Direct Fluorescence...

...WPRE-SV40 124650 AAV9 pAAV-CamKIIa-C1V1(t/t)-mScarlet-KV2.1 55645 AAV5 pAAV-hSyn Con/Fon hChR2(H134R...

...451 (2008): 33-42. PubMed PMID: 19185711. Rosado, Carlos, et al. "Rosella: a fluorescent pH-biosensor for...

...increase efficiency of insertion and gene replacement. Care to share your favorite CRISPR tool or resource? ...

... Randall Carter...

...analysis and sharing whatever she knows with whoever cares to listen. In March 2020 she will start her own ...

...machinery) along with the viral plasmid containing the cargo of interest, and HEKs will produce and package viral...

... MG, Yang D, Miller TE, Feldman J, Hauser BM, Caradonna TM, Clayton KL, Nitido AD, Murali MR, Alter G,...

...in a 50 mL conical tube and dispose after use. Carefully pour the filtered and diluted cell culture supernatant...Resin will appear compacted after centrifugation. Discard the flow through into a waste container. Add 5 ... 2 min to remove PBS. Repeat Steps 30–31 3x , discarding buffer from the collection tube each time. Place...Centrifuge at 1000 x g for 2 min to collect the sample. Discard column after use. Determine antibody concentration...min at RT . Centrifuge at 3100 x g for 8 min . Discard the flow through into a waste container. Add the...times to mix. Centrifuge at 3100 x g for 8 min . Discard the flow through into a waste container. Repeat...additional times to ensure a full buffer exchange. Discard the flow through into a waste container. Fill the...

...developed and characterized by Stanley Perlman, of the Carver College of Medicine and Paul B. McCray, from the...

...background in our study - so you might want to be careful with these clones (and help us figure out what ...

...with the outside surface of the exosome or are carried inside the vesicle, which may also contain other...

...-resistance is necessary to improve tuberculosis care and control. In her current research, Sheen aims...

...methods section of your publication. You should carefully read and follow the journal’s specific author ...

...to create a single library containing fragments carrying sample-specific barcodes. Rather than continuing...

...Data Sheet by RSS-ICTD Amino Acid Structure Quiz/Card by Andrey Solovyev Lab Help by Kris Shamloo These...

...near the target site.” pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (124651-AAV9) Recombinase AAV Targeting...

...Serotype Name 124651  AAV9  pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 124603  AAV9  pAAV-EF1a-DIO-ChrimsonR-...

... These travel awards are intended to help early career scientists attend a conference of their choice ...

...of SciCommSummer Scicomm Series: Modes of CommunicationCareers in Science Communication: Science Writing...

... Carissa Fish...

...incredible place.” Doench has spent most of his research career working on miRNAs and RNAi. He first heard about...

...will have to learn and stretch a little, but be careful not to go too far. Is the assignee interested...

...CRISPR plasmid will have all desired genomic edits carried out by Cas9. Multiplexing applications include ... and delivered to specific genomic locations to carry out unique tasks. We’ll discuss more Cas9 fusion...location in the genome, where the transposase then carries out integration of donor DNA. Both types of CASTs...with the sequence, so the edit is not considered “scarless”. Figure 7: Overview of CRISPR transposases Browse...that displays improved specificity, and editors carrying an ADAR truncation that retain RNA editing capabilities... Yang, L., Esvelt, K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013)...Jaenisch, R. (2013). One-Step generation of mice carrying mutations in multiple genes by CRISPR/CAS-Mediated...

...a transformed mesophilic, for example E. coli, carrying a plasmid expressing a protein of interest). Mesophilic...

... through Addgene. These plasmids can be used to carry out any biochemical and biophysical study involving...

...cancer variants derived from analysis of lung adenocarcinoma genomes (Lung Cancer Mutant Collection) or ...

... system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the DNA sequence, which...

...keep track of what works, and what does not work. Careful documentation will keep you on task and will prevent...

...obvious component of a biosensor is the thing that you care about within your cellular or animal system. This...

...Additional Resources on the Addgene Blog Science careers Plasmids CRISPR Viral vectors Fluorescent proteins...

...other AAV. Therefore, one should aim to keep their cargo below 4.4kB (with the ITRs bringing total genome...

...  At some point in your long and illustrious lab career, you will likely accidentally leave your antibody...

...domain.   Check out our new Isotype trading cards!     References and resources References Janeway...

...of molecules including proteins, lipids, and carbohydrates, in this blog post we’ll focus on antibodies...

...DREADDs were engineered from members of the human muscarinic receptor family and are activated by small molecule...

...result in a high number of background colonies carrying the unmodified plasmid. For insertions, it’s possible...

...differentiation between blue and white colonies. Take care in making your plates: X-gal is light and temperature...

...laboratory animals during in vivo research and the CARE guidelines for case reports are two sets of guidelines...

... die unless they have incorporated the plasmid carrying the required gene.  The table below lists some...

...dissolved in dH 2 O. *Carbenicillin can be used in place of ampicillin. Carbenicillin is more stable, so ...Protocols Cloning Protocols Introduction Plasmids can carry one or more antibiotic resistance genes, which confer...resistance to a specific antibiotic to the bacteria carrying them. The presence of an antibiotic resistance...that antibiotic - usually conferred by a plasmid carrying the antibiotic resistance gene. The following ...100 mg/mL 100 µg/mL Bleocin 5 mg/mL 5 µg/mL Carbenicillin* 100 mg/mL 100 µg/mL Chloramphenicol 25 mg/mL...

...of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast segment...

...incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna, Joanne Kamens, Lenny Teytelman, April...

...necessary result of the #MeToo movement. We are carefully attentive to genuine inclusion, but not worried...

...different plasmid backbones that varied in size or carried different genes. In these cases, gene expression...