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Showing: 181 - 200 of 435 results

  2. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...optogenetic or fluorescent tool with magnets would enable new research and applications that don’t yet exist...Gaykema, R. P., Spano, A. J., Beenhakker, M. P., Kucenas, S., Patel, M. K., Deppmann, C. D., & Güler, A....

  4. Tips for arabidopsis transformation

    Type
    Blog Post
    ...complementing mutant phenotypes. Arabidopsis is amenable to the floral drip or dip transformation method...at Stanford University. She studies cell fate maintenance in the stomatal lineage and has made countless...

  6. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    ...Cas13, REPAIR is the first CRISPR-based system to enable precise RNA editing. The addition of RESCUE further.... "Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature...

  7. DNA Purification Without a Kit

    Type
    Blog Post
    ...bacteria and denature the DNA and proteins in solution. The pH is then lowered using a renaturing solution...

  8. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    ...Payen E, Negre O, Wang G, Hehir K, Fusil F, Down J, Denaro M, Brady T, Westerman K, Cavallesco R, Gillet-Legrand... Answered Learn How Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening Read about Genome-Wide...

  9. CRISPR Antimicrobials

    Type
    Blog Post
    ... which may also induce cell death. CRISPR also enables increased specificity - rather than targeting a...antibiotics, but the field is currently undergoing a renaissance. Yosef et al. designed a two-phage CRISPR system...

  11. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...very flexible cleavage system. LwaCas13a is also amenable to multiplexing; expressing 5 gRNAs simultaneously.... "Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection." Nature...

  12. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    ...several years ago – recently collaborated with Senator Barbara Boxer to introduce the Alan Reinstein Ban... Ban Asbestos Now Act of 2016 in the U.S. Senate. If this bill passes, it will put an immediate stop to...

  13. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ... courses of treatment with antibiotics These scenarios expose bacterial populations to antibiotics at...counter-intuitive considering the other three scenarios, but a short treatment regiment might wipe out...

  15. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ..., … Li, D. (2024). Adenine transversion editors enable precise, efficient A•T-to-C•G base editing in mammalian...optimization of a uracil-N-glycosylase variant enables programmable T-to-G and T-to-C base editing. Molecular...

  16. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...retains important functional properties, and is amenable to a wide range of biophysical assays (Kroupova...low-expression proteins of interest) and mScarlet-I3 for scenarios requiring rapid maturation and prolonged imaging...

  18. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...Proper controls provide the constant variables that enable the correct interpretation of the effect of the...transfection control measures transfection efficiency and enables observation of any effects of the transfection ...

  19. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also called TDH3 ... expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length version is...

  20. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...demonstrated excellent counter-selection which enabled very efficient recombineering.     We were inspired...CRISPR/Cas9 system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia...
Showing: 181 - 200 of 435 results