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Showing: 181 - 200 of 621 results

  2. Designer PUF Proteins for Any RNA Target

    Type
    Blog Post
    .... Get the PUF Assembly Kit! Tips and tricks For those interested to try the PUF Assembly Kit out for themselves...tool…designer PUF proteins! A newly available PUF Assembly Kit makes it possible to devise RNA binding proteins...factors (PUF). In a single step, researchers can now assemble designer PUF domains for RNA specificity engineering... Reference Abil Z, Denard CA, Zhao H.  Modular assembly of designer PUF proteins for specific post-transcriptional...

  3. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    ...flow of electric charges across cell membranes and maintain membrane potential in response to visible light...across the axon's membrane: once a certain number of positive ions crosses the cell membrane, a threshold ...rhodopsins enable ions to flow across the axon’s membrane, thereby controlling neural activity (Bernstein...Stoeckenius. "Rhodopsin-like protein from the purple membrane of Halobacterium halobium." Nature new biology...

  4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...also encodes the Assembly-Activating Protein (AAP) and the recently discovered Membrane Associated Accessory... AAV genome and docks with the genome onto an assembled capsid. Then Rep52/40, which also bind the capsid...generate the three VP proteins. The three VP proteins assemble into capsid at a 1:1:10 ratio (VP1:VP2:VP3). There...reading frame (ORF). While AAP promotes virion assembly, the role of MAAP has not been clearly defined...

  5. FlipGFP, a novel fluorescence protease reporter to study apoptosis

    Type
    Blog Post
    ...apoptosis reporter, ZipGFP, which utilized the self-assembling split GFP (GFP1-10 and GFP11) to increase fluorescence...animals. To do this they modified a different self-assembling split GFP (GFP1-9 and GFP10-11) by flipping a...rectify this the Shu lab first improved the self-assembling capability of sfCherry approximately 20 fold ...FlipGFP to study apoptosis in HeLa cells, zebrafish embryos, and Drosophila. They treated HeLa cells expressing...the spatiotemporal dynamics of apoptosis during embryo development. GFP fluorescence increased over time...

  6. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...three different membrane-targeting flavors – PDGFR, GPI, and SGZ, to improve neuronal membrane trafficking...segment (PDGFR TMD) that anchors the sensor to the membrane and displays it on the extracellular side. In .... PDGFR is a C-terminal fusion to the PDGFR transmembrane domain in the mammalian expression pMinDisplay...glycosylphostidylinositol anchor. SGZ contains a PDGFR transmembrane domain, followed by a modified form of the cytosolic...

  7. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...they hear in their classes,” she said, “but they remember the moments when they discover something new for...focused on an emerging scientific field, BioBuilder embraces new opportunities for scientific exploration, ... Lab is a fully equipped, state-of-the-art lab embedded in LabCentral’s community of innovators that opened... Blog Synthetic Biology and the Importance of Assembly Standards Synthetic Photobiology: Optogentics ...Posts Resources on Addgene.org Guide to SynBio Assembly Standards Find Plasmids for Creating and Controlling...

  8. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ...Receptor: GPCRs are also known as seven-(pass)-transmembrane domain receptors, due to the seven alpha helices...helices that transverse the cellular membrane. The receptor recognizes the appropriate ligand to activate...proteins on the intracellular side of the cell membrane. Heterotrimeric G proteins are important molecular...kit at Addgene! TRUPATH: Bryan Roth’s lab also assembled a collection of plasmids to elucidate the role...DREADDs) (Roth, 2016). DREADDs were engineered from members of the human muscarinic receptor family and are...

  9. CRISPR Pooled gRNA Libraries

    Type
    Collection
    ...164084 Knockout Human Sabatini 3rd 10 7,501 Human Membrane Protein Activation Library 113345 Activation Human...

  10. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...cell divisions of the C. elegans embryo when all essential embryonic genes are individually depleted via...reminded of the extent that C. elegans researchers embrace open science and share resources and tools. That...flybase. microPublication is run and maintained by members of the worm community but there are Drosophila ... lab. SapTrap is a reagent toolkit and plasmid assembly line for the creation of CRISPR targeting vectors...

  11. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...Cultured rat hippocampal neuron expressing pan-membrane ASAP5. Solid blue circle marks the initiation ...via the Kv2.1 targeting motif as well as a pan-membrane construct without the Kv motif. Altogether, if...kinetics and high responsivity near the resting membrane potential, ASAP5 is a great new option. Find ASAP5...specifically binds to active (GTP-bound) Gα. Gα-YFP assembles into functional heterotrimers with endogenous ...optional accessory plasmids, the collection targets members of all four G protein families: Gs, Gi/o, Gq/11...

  12. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    ...fundamental takeaway. Writing these points will help you remember what to say and provide your audience with a clear...venue and professionalism of the conference, and remember to wear comfortable shoes, as you will likely ...your breath before diving in to the next figure. Remember your outline: Instead of getting bogged down in... the minutia or coming off as a broken record, remember the outline you created for yourself. Hit the ... Resources on the Addgene website Become of a member of the Addgene depositor community Learn about ...

  13. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...specific RNA site. The EMBO Journal, 17(17), 5201–5213. https://doi.org/10.1093/emboj/17.17.5201 del Solar...plasmid copy number. The EMBO Journal, 20(24), 7323–7332. https://doi.org/10.1093/emboj/20.24.7323 Resources...post was originally written by Jason Niehaus in December 2015 and was updated by Emily P. Bentley in June...

  14. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...pic.twitter.com/sIIoIsIFd1 — Henry Lab (@EvoEmbryo) September 24, 2018   Xenopus tropicalis (Gary Gorbsky..., mix them in a tank, and observe the beauty of embryo development in real time. I was already a biology...nucleus and put it in a fertilized egg to get edited embryos (replacing the nucleus is still very difficult)...female mosquitoes. Now they are trying to replace embryo injection (hard to do, expensive, not every lab...

  15. Bioinformatics at Addgene

    Type
    Blog Post
    ...start using. Prior experimenting with open source assembly algorithms meant that we already had a clear idea... individual steps to automate plasmid sequence assembly, and possible solutions for each in the form of...quality score analysis, k-mer counting, or sequence assembly), but the order and usage of the scripts needs...the freedom to use multiple open-source sequence assembly tools at the appropriate place in our workflow...Tower to augment our pipeline and enable our team members to automate their weekly sequencing tasks. More...

  16. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...Anyone could fill out the form at any time and a member of our Business Development team would review it.... Identify your enemies In Felicia Day’s book Embrace your Weird, Day identifies nine enemies that stand...Frida Kahlo or you’re stuck drawing stick figures, remember your training as a scientist has been building...world. Penguin Publishing Group. Day, F. (2019). Embrace your weird face your fears and unleash creativity...creativity. Gallery Books. Boyes, A. (2019, November 13). 5 mental mistakes that kill your productivity. Harvard...

  17. How to Be an Excellent Trainee

    Type
    Blog Post
    ... place, learning something new. So how can you remember everything? Write it all down! And I mean everything...second rack in the corner freezer sounds easy to remember in the moment….but you’ll probably forget and ...going to each space in order to double-check you remember where the equipment, reagents, and samples are...to ask! Especially for those new to the bench, remember to let your bench mentor know what you don’t know...polite, appreciative, and clear about their needs. Remember, your bench mentor’s training time is time set...

  18. Lentivirus Production

    Type
    Protocol
    ...drifted. Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into sterile tubes. Store...

  19. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...and breed and they have large, easily modifiable embryos, which is ideal for observing and manipulating ...majority of studies involving axolotls focus on embryonics and biochemistry. Though limited at first, advances...humans so they are often used to study vertebrate embryology and cell development. One major resource for ...understand human disease alleles during and after embryogenesis. Addgene has over 600 plasmids that contain ... a Model Organism. News-Medical. Retrieved on September 18, 2020 from https://www.news-medical.net/life-sciences...

  20. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    ...By breaking the transgene into smaller pieces. Reassembly of fragmented genomes is the key to fitting large...full-expression cassette. AAV’s natural ability to reassemble fragmented genomes is the basis of several split...with both part A and part B AAVs, the fragments reassemble and produce the full-length gene. Below are overviews...Vector, Big Gene Transduction: Fragmented Genome Reassembly of Adeno-associated Virus. Molecular Therapy ...of large genes to the retina by dual AAV vectors. EMBO Mol Med 6:194–211 . https://doi.org/10.1002/emmm...
Showing: 181 - 200 of 621 results