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We narrowed to 627 results for: PROC

Showing: 181 - 200 of 627 results

  1. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    ...relaying key points about an unfamiliar topic or lab process. Along with our role as a plasmid repository, Addgene...-by-step instructions to navigate various lab processes.  Our most recent protocol video detailing Streaking...details every stage of the selection and checkout process on Addgene’s website: We’ll be continuously updating...

  2. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...viral vector. To start the AAV packaged on request process, simply navigate to your plasmid of interest. If...the same trusted production and quality control processes as our in-stock service: ddPCR, purity testing...GC/mL for AAV2. There are no quotes or special processes involved; simply request your plasmid of interest...

  3. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    ...the template and primer binding site, increase processivity, or improve thermostability. What came out on...increasing editing efficiencies 2-3-fold. However, the process of double nicking increases indel formation slightly...repair (MMR) complex, to temporarily inhibit this process and promote copying of the edited strand to the...to the numerous innovations that streamline the process of prime editing, the Liu lab contributed an improved...

  4. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...development has caught up with it. The peer review process has been challenged with many closely spaced CRISPR... are trying to solve, and finally how we might proceed in the future. When we think of designing experiments...These functions help speed up the sgRNA design process by removing repetitive tasks and/or by providing...providing features to help with the post-design process such as primer and plasmid design. Examples:  “multiplex...

  5. Plasmid Preps: Different Purity, Different Quantities, Different Uses

    Type
    Blog Post
    ...Transformation grade DNA  Transformation is the process of inserting exogenous DNA in a microorganism. ...contaminants that have been used during the purification procedure. Good indicators of DNA purity are the absorbance...transfection grade DNA. Transfection is the process of inserting an external plasmid in mammalian cells...

  6. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ..., you can submit plasmids at any point in that process and we provide an option to keep your deposit on...making two changes to the policy that will make the process a bit easier and more efficient. Our original policy...impact of your work. Plus, it’s costly for us to process and store materials that aren’t distributed or ...

  7. An Addgene Summer Internship

    Type
    Blog Post
    ...  I applied to Addgene and began the interview process with Amy, who let me know in March that I successfully...crunch and knew that if we were to restart the procedure we would not have enough time. During the following...This report shared my work by breaking down the process of each day, my background, observations, etc. ...

  8. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ... Addgene has no investors or stock. All of our proceeds go into running the repository and serving scientists...curious what specifically drives the decision making process at Addgene, just look to our core values. These...deposit and request plasmids (via web interface, processes, distribution, and customer service) Addgene provides...

  9. Viral Vectors 101: Transductions

    Type
    Blog Post
    ... What is a transduction?  Transduction is the process of transferring foreign nucleic acid into a cell.... Transductions are similar to transfections, processes which introduce foreign nucleic acid into cells...   Figure 1: Transduction to expression process. A viral particle enters a host cell, enters the...

  10. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    ...production process while still ordering viral vectors. Virus production can be a frustrating process that requires...developing ourselves as a knowledge base for the processes of viral production and testing so that researchers...

  11. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    ...Autophagy (Greek for “self-eating”) is a process by which cytoplasmic material, including organelles,...lysosomes for degradation. Autophagy is a dynamic process which involves autophagosome synthesis, delivery...inherent limitations because it turns a dynamic process into a static measurement and it provides limited...

  13. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    ...Award highlights TTOs that accelerate science by processing agreements quickly! If your TTO receives a Golden...transfer professionals: Describe to your TTO how unprocessed agreements slow down your research; provide specific...all future requests/deposits. This reduces the processing time of request MTAs and deposit agreements to...

  14. Don’t Wait To Launch Your Health Science Startup

    Type
    Blog Post
    ...background and/or connections to even begin this process. I am the cofounder and CEO of miLab, a Boston-...innovative ideas in all facets of the entrepreneurial process in order to develop those ideas into commercial...people for guidance.  Before even beginning the process of launching a company, you need to determine whether...

  15. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    ... is to quickly generate a single cell line and proceed to use it for experiments.” - Alex Chavez, researcher...scientists, producing lentivirus is not always an easy process. The cloning capacity of lentiviral vectors (~8.5... genes involved in a wide variety of cellular processes. While these are widely requested and incredibly...

  16. The Crispr Babies Saga Shows the Need for Action, Not More Delays

    Type
    Blog Post
    ...and effective. We do that through a standardized process of good research practice: computer simulations...’s experiments prove otherwise, the scientific process does seem to be working. I would argue that one...He and the CRISPR Babies is that the scientific process, as imperfect as it may be, does work when it is...

  17. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    ...fact, precise light-based control of biological processes is highly useful in other fields, including synthetic...to directly activate and inactivate biological processes. Chemical effectors are suboptimal for a number...intensity and wavelength can be used to study dynamic processes. Conversely, varied input patterns may also be...

  18. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    ...polymerize, allowing the slower exonuclease activity to proceed. By including only one type of dNTP in the reaction... can decide exactly how far the T4 exonuclease proceeds: at the first occurrence of the chosen nucleotide...: Schematic of SLIC. To start the SLIC cloning process, a gene of interest is PCR amplified to add 5′ ...

  19. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...easier with more perspectives contributing to the process Senior people (of which there are never enough,...during the meeting.  Scientists do better with process, so don’t just get together and chat. Have an agenda...started. Have regular meetings. Experiment with the process. Have each person write a Development Plan. Set...

  20. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...effect of protein synthesis and degradation. These processes control the abundance of proteins in cells. Homeostatic...incorporation of puromycin into peptide chains." Proceedings of the National Academy of Sciences 51.4 (1964...puromycin of amino acid incorporation into protein." Proceedings of the National Academy of Sciences 45.12 (1959...
Showing: 181 - 200 of 627 results