...Connie Cepko, Harvard Medical School Ali Cetin and Shenqin Yao, Allen Institute Ben Deverman, Broad Institute...

...of DNA into prokaryotic chromosomes - Endy and Shearwin Labs Ralstonia-GG-Kit : Assembly of Ralstonia ...

...Fire Lab. Available with and without PATC, which diminishes epigenetic silencing in the germline. A "FLP-...

...uniqueness of CRISPRi, as compared to several recently published works on applying the wild-type CRISPR system ...

...52255 pUC119-gRNA U6 PCR template none S. pyogenes Sheen 51295 pRGEB31 rice snoRNA U3 BsaI none S. pyogenes...

... and a new pSerOTS. Recently the Rinehart lab published a new strain of recoded E.coli (rEcoli XpS 192872...

...Promoter Project (Link opens in a new window) was established to advance research and therapies for brain disorders...

... of Cas9 in vivo and ex vivo , the Zhang Lab established Cre-dependent and constitutively expressing Cas9...

...ligand DKFZp686F2250, KL-1, Kitl, MGF, SCF, SF, SHEP7 KL klotho - LACRT lacritin MGC71934 LECT2 leukocyte... stimulating hormone receptor) MGC14337, MSH-R, SHEP2 MC2R melanocortin 2 receptor (adrenocorticotropic...Schaefer H, Chen J, Hu Z, Zalocusky KA, Shankar RD, Shen-Orr SS, Thomson E, Wiser J, Butte AJ. 2018. ImmPort...

...platform, termed SHERLOCK, can identify target sequences using a fluorescent reporter. In SHERLOCK, a quenched... the presence of that specific target. SHERLOCK (and SHERLOCKv2) allows for greater amplification and ...bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the Feng Zhang lab , is a Cas13a-based...flow strips. Other Cas enzymes can be added to SHERLOCKv2 to detect multiple targets. For example, Cas12...Nishimasu, H., Ran, F. A., Hsu, P. D., Konermann, S., Shehata, S. I., Dohmae, N., Ishitani, R., Zhang, F., & ...system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman, E., Younger, S. T., & Rinn...

...The insert is typically the genetic element one wishes to study using a particular plasmid. Promoter Region...expression of an endogenous gene. This is frequently accomplished through expression of an shRNA targeting the...edit genomes. Genome editing is most commonly accomplished using CRISPR technology. CRISPR is composed ...concentrations. We suggest checking your plasmid's datasheet or the plasmid map to confirm which antibiotic...with a different fluorophore so they can be distinguished from one another. Similar to DNA replication...

... Prigge M, Schneider F, Davidson TJ, O'Shea DJ, Sohal VS, Goshen I, Finkelstein J, Paz JT, et al. 2011...Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I, Thompson KR, Deisseroth K. 2010 Molecular and... Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M, Fenno LE, Gradinaru...

...the number of signaling molecules that can be distinguished in your application. The indirect detection ...Cross-link the samples with formaldehyde or UV light. Shear the samples using sonication to break DNA up into...protein. Micrococcal nuclease digestion is used to shear the DNA in these assays. This method is suitable...specific to your antibody and assay may already be published. If it is not, you’ll need to validate the antibody...

...onto the insert of interest using PCR. This is accomplished by PCR amplifying the insert sequence using ...round of reverse transcription. This deletion abolishes transcription of the full-length virus after it...

...plasmid(s) online (left), choose to submit published or unpublished plasmid(s). Fields marked with an asterisk...submitting plasmid(s) using a spreadsheet (right), click the “Download Deposit Spreadsheet” button to download ... the online form you can submit "Published plasmids" or "Unpublished or pre-publication plasmids" and ...plasmids. You can submit "Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that...(for online submission) or "Download Deposit Spreadsheet" (for emailed submission) in the Deposit menu..."Submit Online Deposit" or "Download Deposit Spreadsheet". Start your plasmid deposit There are two options...Choose to submit plasmid(s) online or by using a spreadsheet. The plasmid information can be entered using...

...Gordon Fishell New viral prep pAAV_BiSSTe10_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiCHATe27_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiVIPe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiLAMP5e3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiPVe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiPVe3_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...Gordon Fishell New viral prep pAAV_BiSSTe4_ChR2_mCherry AAV-PHPeB Optogenetics Gordon Fishell New viral...

...deeper imaging into thick tissues. Light Sheet Microscopy Light Sheet Microscopy typically utilizes a configuration...evanescent energy field. Lightsheet microscopes that project ultra-thin sheets of excitation light through...tissues need to be imaged via Lightsheet microscopy. Although not all lightsheet microscopes can cover samples...1); similar to an MRI or CT scanner. This is accomplished by focusing excitation light to a point in the... bleaching) to a single focal point. This is accomplished by using, for example, red light instead of ...configuration of two or more objectives to create a thin sheet of excitation light that propagates perpendicular...sectioning (5). One such example is the lattice lightsheet that projects a number of thin cone-shaped beams...

...sharing policy. Since early March, the open access publisher has required authors to include a note as to where...result of consultations between researchers and publishers. Nonetheless, the initial release caused a storm...increasing the ability of reviewers (and, once published, anyone) to detect possible errors in assumptions...their own data? A distinct (perhaps similarly impoverished lab) might apply different or novel tools and... video, generating huge amounts of data. When published, this data is usually condensed into a statistically... data are likely to improve research. What is published is typically a tiny fraction of what was actually...data accessibility and methodological detail. Publishers have also contributed to this issue by requiring...

...research project, the vast majority of what gets published in scientific journals flies under the radar and...have time to keep up with all the research being published on our own topic. Only on occasion do we then ...   Video abstracts about research, especially published research, have grown in popularity over the last...this: “In terms of total view count and videos published, YouTube reported a total of 865,995 views for...online article downloads – especially if they are published in open access journals.   Check Out Addgene Videos...Facebook is continuing its growth, and while many publishers and researchers have Facebook pages, few share... leading the way, the future of complementing published research with video overviews was growing quickly...

...Deisseroth 83895 AAVrg pAAV-hDlx-Flex-GFP-Fishell_6 Gordon Fishell   Optogenetics Optogenetics continues...Cepko 83898 AAVrg pAAV-mDlx-ChR2-mCherry-Fishell-3 Gordon Fishell   Chemogenetics Chemogenetics offers...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 83897 AAVrg pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Controls Plasmid...pAAV-mDlx-GFP-Fishell-1 Biosensors Plasmid Serotype Name 83899 AAVrg pAAV-mDlx-GCaMP6f-Fishell-2 ...