...multichannel pipette set to 5 µL. For dilutions 6–8, use the 20–200 µL multichannel pipette set to 50 µL. For...dilutions, use the 20–200 µL multichannel pipette set to 90 µL and mix by pipetting the liquid up and down...ddPCR plate onto a chilled 96-well freezer block and set aside in the droplet generation BSC to cool. Prepare... Droplet Reader. Open the QuantaSoft software to set up a new plate layout. Designate the sample name,...

...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...-day experiment in the lab. You have two ways to set up the experiment. The first way, it will take you... project (with metrics if possible) and, at the outset of a task, I outline the activities that are needed...

...are ready to begin the screening process. Want to set yourself up for a screening success? Before you start...look for off-target changes. This method requires a set of control cells to compare the sequencing reads ...

...newly synthesized DNA strand. Each degenerate primer set contains three consecutive randomized bases (NNN)...NNN) at a given codon site and multiple primer sets tile across the protein or region of interest. A low...

...from someone seeing me speak in a non-interview setting. There are many resources on self-promotion (how...of this article will be talks for non-academic settings. Many of these guidelines will apply to any type...

...contamination with RNase leading to degradation that can set you back days. Working with plasmid DNA is much easier...pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple PCR with ...contains humanized Cas9 expression cassette and sgRNA expression cassette. The target sgRNA can be cloned...of validation for DSB mediated EGFP expression cassette reconstitution. When the target sequence was digested...took place and reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP target plasmid and pX330-sgRNA...

...energy, Martí Aldea and collaborators have created a set of “innocuous tags” (inntags) less likely to alter...

...while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help scientists...

... recently deposited “Mobile-CRISPRi” plasmids, a set of modular CRISPRi tools that can be used in diverse...

...endeavor.  Break the process down into steps and set aside time during the week to tackle each one.  Get...

...careers. Failure to strengthen the talent pipeline sets the world up for reduced impact of scientists and...spearheaded by Cynthia Fuhrmann at University of Massachusetts Medical School. It has been supported by a Steering...

...  Cloning grade DNA Molecular cloning includes a set of techniques that are used to insert recombinant...

...the biggest hurdles for scientific sharing. So we set out to streamline this process. We take pride in ...

... Mix & Go! E. coli Transformation Kit and Buffer Set  from Zymo Research to make competent cells because...

...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ...library that contains up-to-date information and data-sets, as well as an interactive gRNA viewer. The Moffat...address this problem, Csaba Pál’s lab has created a set of vectors that allows one to use the MAGE method...method in unmodified bacterial species (4). This set of plasmids (dubbed pORTMAGE) expresses the λ Red recombinase... kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository. The... unique tool with their Yeast Prototrophy Kit, a set of plasmids designed to complement unused auxotrophies...Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles...

...in leukemia, the Lowe lab was able to identify a set of mutations that made cancer cells more resistant...systems check out Gerald Rubin’s lab who created a set of modular vectors that can be used to optimally ...

...Goedhart and colleagues in the Gadella Lab devised a set of optogenetic switches for the GEFs ITSN1, TIAM1...controlled phospholipase D (PLD). Now they present a set of variants called SuperPLDs with 10, 30, 50, and...

...specifically developed to thrive in laboratory settings. One of its most attractive features is its stability...easily maintained in a laboratory environment. What sets Arabidopsis apart from other plant model organisms...

... go over the details step-by-step. 1. Digestion Set up restriction digests for your insert (or donor ...of recipient plasmid to insert. It is critical to set up a negative control ligation reaction with no insert...

...approaches to visualize bacteria in an educational setting. The Symposium on ‘Creative Approaches to Presenting...