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We narrowed to 303 results for: crispr cas9

Showing: 181 - 200 of 303 results

  1. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...accounts with  Addgene. We now have the incredible CRISPR/cas9 tools at our disposal to to modify the worm genome...would be useful to our model  so that we can use CRISPR/cas9 to build the mutants, analyse their motor behaviour... crispr...

  2. CRISPR Plasmids - Empty gRNA Vectors

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    Collection
    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires... CRISPR Empty gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids... plasmid contains Cas9 and if so, which function of Cas9. We have plasmids with Cas9 that can cut, activate...independent U6 promoters and Cas9 from CBh promoter. Ventura A CRISPR/Cas9 toolkit for multiplex genome...cassettes are assembled into a Cas9 expressing vector. Yang A Robust CRISPR/Cas9 System for Convenient, High-Efficiency...accessory pack is available for dCas9 and FokI-dCas9. Yamamoto Multiplex CRISPR/Cas9-based genome engineering...System Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI pCRISPR 42875 Bacteria BsaI none S. pyogenes...

  3. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post
    ...adeno-cre. AAV-based gene editing compared to CRISPR editing CRISPR, of course, has started a revolution in ...whether to use AAV or CRISPR for a given experimental approach. First, unlike CRISPR, the AAV-based approach...combining CRISPR with AAVs for improved genome editing is hampered by the fact that even small Cas9 homologs...PMCID: PMC3277219. 6. Ohmori, Tsukasa, et al. "CRISPR/Cas9-mediated genome editing via postnatal administration...combined AAVs with CRISPR systems. Ohmori et al 2017 use a dual vector system composed of SaCas9 and a gRNA in...in gene editing. However, while CRISPR is remarkably effective for introducing non-sequence specific frameshift...proven more challenging to develop high efficiency CRISPR-based approaches for the generation of knockins...

  4. CRISPR Plasmids - Bacteria

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base...Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 42875 pCRISPR BsaI E. coli, ... CRISPR Bacteria CRISPR Plasmids: Bacteria...following CRISPR plasmids have been designed for use in bacteria. Cut Fully functional CRISPR/Cas enzymes... Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of genomic DNA...immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus, avoiding... pneumoniae Kanamycin none, need Cas9 plasmid Marraffini 42876 pCas9 BsaI E. coli, S. pneumoniae Chloramphenicol...

  5. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    Blog Post
    ...-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation method...developed, relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish...systems, and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite these... Resources on Addgene.org Browse our zebrafish CRISPR plasmid collection Read our molecular biology reference...

  6. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type
    Blog Post
    ...editing Read more about CRISPR Learn all the basics about CRISPR in our CRISPR 101 blog series Resources...compatible with all Cas9 and Cas12 homologs that the researchers tested, including SpCas9, SaCas9, LbCas12a, enAsCas12a... Cell Symposia: Gene- and Cell-based Therapies: CRISPR, Stem Cells, and Beyond meeting earlier this month...the DNA. ABEs are somewhat slow and need a strong Cas9 domain to “hold on” long enough to the DNA to induce...Addgene.org Find base editors for your research Read our CRISPR guide Find plasmids for your research ... enAsCas12a, SpCas9-NG, SaCas9-KKH, CP1028-SpCas9, and CP1041-SpCas9. The resulting base editor was named... weakly compatible with many Cas domains beyond SpCas9. In order to expand the gene-editing applications...

  7. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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    Blog Post
    ...A, Li K, Wang B, Jin S, Reiser M, Lockey RF. CRISPR/Cas9 nuclease cleavage combined with Gibson assembly...mixing and matching of parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning...use of Gibson assembly combined with the popular CRISPR technology and is described in a recent publication...restriction enzyme or PCR to make linearized vector, the Cas9 enzyme along with a specific gRNA was used to cleave...second example of using Gibson Assembly along with CRISPR was also described recently (Jiang et al. 2015)...chromosome (up to 100 kb) were specifically cut out via CRISPR and then assembled into a vector using Gibson assembly...homology with the bacterial chromosome fragments. CRISPR cutting was used to circumvent the need to PCR ...

  8. Addgene’s Top 10 Blog Posts from 2014

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    Blog Post
    ...and improved. The CRISPR posts written by our guest bloggers, the experts in the CRISPR/Cas field, have ...Plasmids 101: Yeast Vectors By Marcy Patrick CRISPR-Cas9 FAQs Answered! By Caroline LaManna Plasmids...past year. A quick glance shows that interest in CRISPR continues to grow as the genome editing technique...Popular Posts of 2014 How to Design Your gRNA for CRISPR Genome Editing By John Doench (Guest Blogger) ...

  9. CRISPR Plasmids - Zebrafish

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Zebrafish CRISPR Plasmids: Zebrafish...following CRISPR plasmids have been designed for use in zebrafish. Cut Fully functional CRISPR/Cas enzymes...Enzyme(s) Delivery Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 46759...none, need Cas9 plasmid Chen and Wente 42250 DR274 T7 BsaI In vitro transcription none, need Cas9 plasmid...CRISPR plasmids that have been designed for use in zebrafish. CRISPR...Zebrafish Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...

  10. Zebrafish Plasmid Collection

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    Collection
    ...Joung Lab CRISPR/Cas9-based conditional mutagenesis in zebrafish - Wenbiao Chen Lab A CRISPR/Cas9 Vector ...modified CRISPR-Cas9 system - Shuo Lin Lab Re-purposed Ac/Ds transgenic system enabling CRISPR/dCas9-interference...Collection CRISPR Please refer to Addgene's CRISPR Guide for a general introduction to CRISPR technology...for Targeted Integration Using CRISPR/Cas9 - Jeffrey Essner Lab CRISPR-Cas/RNA-Guided Nuclease (RGN) expression...the laboratory use of zebrafish. CRISPRz - A database of CRISPR/Cas9 target sequences that have been experimentally...morpholinos, zinc finger nucleases, TALENS, and CRISPR/Cas9-based gene editing make the zebrafish a stalwart... or the zebrafish CRISPR resources for a full selection of plasmids expressing Cas9 and empty gRNA backbones...

  11. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...repurposed for CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too...more of these kits, as there are many options! CRISPR CRISPR-Cas can harness the power of MoClo to expand...including conditional gene expression, multiplexed CRISPR, and chromosomal integration of larger expression...targetability and effectiveness. Perhaps the most intuitive CRISPR MoClo application is to assemble multiple unique...unique sgRNAs into a multi-TU targeting construct (Cas9, sgRNA, reporter, etc.). This allows for the multiplexing...edit optimization, and more. While there are many CRISPR-Cas libraries and tools available for certain model...biologists, Addgene has not one, not two, but three CRISPR MoClo kits for plants that work for both monocots...

  12. CRISPR Plasmids - Tagging

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Protein Tagging CRISPR Plasmids...Read our CRISPR 101 post for more information about HDR/repair template design. How to use CRISPR to tag...for a CRISPR-based system to add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system...system consists of two components: An all-in-one CRISPR/Cas9 vector based on the Zhang lab's PX330 , simultaneously...pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA, & FBL-specific...SapTrap CRISPR/Cas Toolkit SapTrap is a modular toolkit from Erik Jorgensen's lab for building CRISPR targeting...

  13. Finding nucleic acids with SHERLOCK and DETECTR

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    Blog Post
    ...the last few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop... DNA Endonuclease Targeted CRISPR Trans Reporter Cas12a (Cpf1) is a CRISPR Cas variant that can also cleave...Resources on Addgene.org Checkout our CRISPR guide page Browse the CRISPR Collection ...develop the method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR) (Chen et al., 2018). Both...Together SHERLOCK and DETECTR demonstrate the power of CRISPR-based diagnostics. SHERLOCK- Specific High Sensitivity...Abudayyeh et al 2016). Cas13 can be guided by a single CRISPR RNA (crRNA) to cleave ssRNA or mRNA. It also exhibits...reporters for each enzyme. In addition, Csm6, a CRISPR type-III effector nuclease, can be used in conjunction...

  14. CRISPR Plasmids - Prime Edit

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    Collection
    ... CRISPR Prime Edit CRISPR Plasmids: Prime Edit Browse CRISPR Plasmids By Function ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Prime...contains Cas9. ID Plasmid Expression System Promoter Guide RNA Type Cloning Enzyme Co-expressed Cas9 Selection...transcriptase (RT) is fused to the C terminus of Cas9 H840A nickase. The fusion enzyme is capable of installing...template. During prime editing, the pegRNA directs the Cas9 nickase to the target sequence where it nicks the...nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used in PE2...PE6 - small prime editors with optimized RT and/or Cas9 domains PE7 - addition of RNA-binding exonuclease...

  15. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ...Resources and references References Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...screening, and validation of CRISPR libraries. The Genome Engineering using CRISPR/Cas Systems Forum- Moderated...Lentiviral CRISPR Libraries- A nice overview of what to consider when using pooled lentiviral CRISPR libraries...Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...but we do distribute several of our most popular CRISPR pooled libraries and one of our most popular barcoding...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Curr Protoc Mol Biol. 2016 Jul 1;115...

  16. CRISPR Plasmids - Yeast

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    Collection
    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Yeast CRISPR Plasmids: Yeast Browse ... following CRISPR plasmids have been designed for use in yeast. Cut Fully functional CRISPR/Cas enzymes...) Validated In Resistance Co-expressed Cas9 Depositing lab Cas9 species = S. pyogenes (PAM = NGG) 49014... Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of genomic DNA...immunoprecipitate FLAG-tagged Cas9. Design your gRNA sequence to direct dCas9 to a specific locus, avoiding...CRISPR plasmids that have been designed for use in yeast. CRISPR...

  17. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    ...discovery. CRISPR is a great example (the top requested plasmids from 2015 and 2016 were CRISPR plasmids...lentiviral vectors, many of which are used to deliver Cas9 and other genes to mammalian cells. For this reason...ubiquitin ligase, cellular senescence, autophagy, CRISPR, and so much more (find the full, searchable list...

  18. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...used  (Thomason et al., 2014). In recent years, CRISPR-Cas9 technology has also aided in the creation of ...15109831. Pyne, Michael E., et al. "Coupling the CRISPR/Cas9 system with lambda red recombineering enables...genomes using exogenous enzymes: Lambda red and CRISPR Developed over the past 20 years, Lambda Red recombineering... "RNA-guided editing of bacterial genomes using CRISPR-Cas systems." Nature biotechnology31.3 (2013): ... and Kristala LJ Prather. "The no-SCAR (Scarless Cas9 Assisted Recombineering) system for genome editing...blog Read our Plasmids 101 blog posts Find our CRISPR blog posts Learn more about Lambda Red recombineering...

  19. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    ...with the gRNA), application, cas9 species, PubMed ID, and depositor. The CRISPR applications we currently...Design Your gRNA Use the CRISPR Software Matchmaker Browse Alll of Our CRISPR Posts Additional Resources... Browse gRNA Design Tools Read Our CRISPR Guide Pages Find CRISPR Plasmids  ...visualize, nick, purify, tag, scaffold, CRISPR-display, and dCas9-FokI. This table is both sortable and ... one.  At Addgene, over 120 labs have deposited CRISPR reagents, including many gRNA-containing plasmids...articles. If you’re targeting your favorite gene with CRISPR, using one of these validated gRNAs can save you...target selection and gRNA design are essential for a CRISPR experiment.  The selection process is often time...

  20. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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    Blog Post
    ...time CRISPR user? Check out our tips for 1st time CRISPR users Learn how to Choose the Best Cas9 Variant...Find Other Cas9 Variants for Purifying Genomic Regions of Interest Here Brush Up on Your CRISPR Background...the resolution, the Xu lab created a dCas9-based CAPTURE (CRISPR Affinity Purification in situ of Regulatory...at hundreds of loci at once (Liu et al., 2020). CRISPR Affinity Purification in situ of Regulatory Elements...Background with Addgene’s CRISPR Guide Page ... crispr...co-expressed, the gRNA targets the dCas9 to the loci of interest and dCas9 is biotinylated by BirA. From here...
Showing: 181 - 200 of 303 results