CRISPR Plasmids and Resources
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...Request ready-to-use viral preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of...
We narrowed to 192 results for: lenti
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Using AAV for Neuronal Tracing
TypeBlog Post...). AAV does not have a membrane envelope like lentivirus, but instead is contained by a capsid which is... -
COVID-19 Resources
TypeCollection... lab - Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee... -
Neurodegeneration Research Collection
TypeCollection... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian... -
Fluorescent Protein Guide: Biosensors
TypeCollection... Mitophagy Dual-emission mitophagy biosensor (lentiviral expression of pH sensitive mt-mKeima) Spatiotemporal... -
Immunology Research Plasmids and Resources
TypeCollection...labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System Kit : A modular and ...system that can be used to construct complex lentiviruses for modification of mammalian cells with a single... -
Gamma-Retroviral Vector Guide
TypeGuide...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide...available at Addgene. Gamma-Retrovirus Versus Lentivirus Lentiviruses and gamma-retroviruses fall under the Retroviridae...main difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting...that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and ...containing only the necessary packaging genes, while lentiviruses also contain accessory genes specific to each...gamma-retroviruses may not be efficiently packaged by lentiviral packaging systems, and vice versa. Gamma-retroviral... 293T (HEK293T) cells (Figure 3A), similar to lentiviral packaging methods . The three plasmids described... -
CRISPR Guide
TypeGuide... libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available... -
Plan Your Experiment
TypeGuide...production, see our viral vector guides . Lentiviral vectors Lentiviral vectors are a popular option for producing...types like primary cells. For CRISPR delivery, lentiviral and adeno-associated virus (AAV) vectors are ...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...we don’t discuss CRISPR screens in this guide, lentiviral vectors are a common choice for conducting genome-wide...CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages...advantages for viral vector delivery. Both lentiviral and AAV vectors are safe to use in the lab, but ...into your viral vector) is smaller than that of lentivirus. Thus, AAVs are generally only compatible with... -
Guide to Using Pooled Libraries
TypeGuide...cells of interest. With others, notably pooled lentiviral plasmid libraries, the plasmids must first be...Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...delivering as virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells...libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout... -
Science Guides
TypeGuide...that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus... -
Molecular Biology Reference
TypeGuide...plasmids to create viral particles (most commonly lentiviral, retroviral, AAV, or adenoviral particles) that...