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We narrowed to 194 results for: lenti

Showing: 181 - 194 of 194 results

  1. Plasmid Collections

    Type
    Collection
    ...Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus...

  3. CRISPR Plasmids and Resources

    Type
    Collection
    ...Request ready-to-use viral preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of...

  4. Retrovirus Plasmids

    Type
    Collection
    ...Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...

  6. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...). AAV does not have a membrane envelope like lentivirus, but instead is contained by a capsid which is...

  8. Immunology Research Plasmids and Resources

    Type
    Collection
    ...labs of William Hahn and David Root . Multiple Lentiviral Expression (MuLE) System Kit : A modular and ...system that can be used to construct complex lentiviruses for modification of mammalian cells with a single...

  9. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide...available at Addgene. Gamma-Retrovirus Versus Lentivirus Lentiviruses and gamma-retroviruses fall under the Retroviridae...main difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting...that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and ...containing only the necessary packaging genes, while lentiviruses also contain accessory genes specific to each...gamma-retroviruses may not be efficiently packaged by lentiviral packaging systems, and vice versa. Gamma-retroviral... 293T (HEK293T) cells (Figure 3A), similar to lentiviral packaging methods . The three plasmids described...

  10. CRISPR Guide

    Type
    Guide
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...

  11. Plan Your Experiment

    Type
    Guide
    ...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable,...high efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector...

  12. Guide to Using Pooled Libraries

    Type
    Guide
    ...cells of interest. With others, notably pooled lentiviral plasmid libraries, the plasmids must first be...Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...delivering as virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells...libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...

  13. Molecular Biology Reference

    Type
    Guide
    ...these plasmids to create viral particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that...viral service offers select ready-made AAV and lentiviral particles. Visit our viral service page to learn...

  14. Science Guides

    Type
    Guide
    ...that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus...
Showing: 181 - 194 of 194 results