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We narrowed to 470 results for: mag

Showing: 181 - 200 of 470 results

  1. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...Imagine being able to determine whether two proteins are within 10 nanometers of each other, or measure...FRET! Read on to find out more about this amazing imaging technique and find further tips for using FRET ...demonstrated the use of single-molecule FRET for imaging biomolecules in live cells (Sustarsic and Kapanidis...

  2. Plasmids 101: Modular Cloning

    Type
    Blog Post
    ...functional units which are transcribed together. Image courtesy of Robert Hurt. Understanding how individual... Figure 2: The three assembly levels of MoClo. Image courtesy of Rob Hurt. What makes MoClo systems...You don’t have to start from scratch! As you can imagine, there is an array of distinct goals within metabolic...

  3. Lentivirus Production

    Type
    Protocol
    ...growth. Left panels: bright field images; right panels: GFP channel images. Last reviewed on: August 2, 2023... Hazardous waste container pH meter Stir plate Magnetic Stir Bar Reagents DMEM high glucose, Corning 10...Heat-inactivated FBS 1x PBS pH 7.4 without calcium or magnesium, Corning 21-040-CV (cations can affect the attachment...

  4. DNA Purification Without a Kit

    Type
    Blog Post
    ... Figure 1: Purify DNA using glass filters. Image: Kim and Morrison, 2009. This method provides...RNA can be amplified. Compared to a commercial magnetic bead-based kit, this cellulose-based method was...Steps to purify DNA using a cellulose dipstick. Image: Zou et al., 2018. Regeneration of silica columns...

  5. Droplet Digital PCR for AAV Quantitation

    Type
    Blog Post
    ...droplets have been read, the software outputs an image of the fluorescence amplitude measured for each ... should have very few positive droplets. In the image to the right, there is approximately 1 positive ...  References Furuta-Hanawa, Birei, Teruhide Yamaguchi, and Eriko Uchida. "2D droplet digital PCR as ...

  6. MXS Chaining

    Type
    Blog Post
    ...fluorescent proteins and the availability of powerful imaging technologies, the combination of multiple fluorescent...was designed to create plasmids for fluorescence imaging or flow cytometry applications in mammalian cells...MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems...

  7. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...distinguishable thanks to the use of phenol red. Image adapted from Zolotukhin, S., et al. "Recombinant...indicates the location of the purified AAV. Left image adapted from Zolotukhin, S., et al. "Recombinant...harvesting of the purified AAV using option #1. Right image adapted by permission from Macmillan Publishers ...capsid proteins VP1, VP2, VP3; M protein marker. Image adapted from Strobel Benjamin, Miller Felix D., ...

  8. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    ...Dropbox, Box, One Drive, and G Drive. ‘Big’ data like image banks and sequencing files is likely stored in institutional...selectively bring particular items into an ELN – e.g. an image that will be re-used frequently in the group’s research... but also to the associated data (spreadsheets, images, sequencing data, etc.) used and/or referenced ...

  9. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ... run, all markers are read in parallel.  Let’s imagine we’re looking for cells in a sample that are negative...often easier to identify different populations by imagining (or drawing) a quadrant over the dot plot. Created...to see these populations, I suggest using the imaginary quadrant to help (Figure 5). Even though this ...

  10. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ... for imaging applications; better tissue penetration which is useful for therapeutic and imaging applications...frankenbodies, an anti-HA scFv-based probe for fluorescent imaging. Learn more about antibodies  Read about another...

  11. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...virtually” via a webcam. In the office, we use a Black Magic Pocket Cinema Camera 4K. It’s wonderful and simple...zoom, stop/start record, and allow me to see an image.  Remote video: So first things first was getting...HDMI transmitter/receiver, to beam the camera’s image to my remote location, live. After doing a bit of...

  12. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...of pTOX that encode one of two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018...hours, the resulting colonies turn subtly blue or magenta. This is particularly valuable when first optimizing...antibiotic resistant clones. But if they are blue or magenta, they are real! Planning an allelic exchange experiment...

  13. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    ...updated by Susanna Stroik January 24, 2023. DNA damage drives genome instability and contributes to cancer...or it can introduce specific mutations into the damaged DNA if these modifications exist in the template...synthesized sequences are retained by the original damaged DNA molecule. Unlike in DSBR, following strand ...

  14. March for Science

    Type
    Blog Post
    ...gutting 38 EPA programs: draft budget  Science Magazine: A grim budget day for U.S. science: analysis ...analysis and reaction to Trump’s plan Science Magazine: NIH, DOE Office of Science face deep cuts in Trump’s ...Environmental Science and Technology: Crossing The Imaginary Line The New York Times: A Scientists’ March on...

  15. Adenovirus Guide

    Type
    Guide
    ...that responds to general signals of infection or damage. Insertional mutagenesis Random insertion of DNA...

  16. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ...looks like the one found on the left side of the image below. It has a promoter (blue arrow) followed by... using UV light – please note that UV light can damage DNA and that proper personal protective equipment... worn when visualizing using UV as it can cause damage to the eyes and skin. When running a gel for purification...

  17. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    ... have expressed GFP) that I couldn’t get a good image. You could probably also do Western blotting against..., but to do it you would need a pretty good gel image. If you are analyzing short DNA fragments, run PAGE...Primers UCSC Genome Browser: For eploring genomic DNA ImageJ: Free Software for Analyzing Gels ...

  18. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    ...contain many epitopes that antibodies can recognize. Imagine you’re doing a word search puzzle. You’re not always...concentration of cell lysate was added to each well. Image from Sun et al., 2010.   Enzyme-linked immunosorbent...fluorophore-conjugated secondary antibody or Fab. Image from Harden et al., 2014.   In vivo uses For ...

  20. Centrifugation

    Type
    Protocol
    ...they are distributed evenly around the center (see image to right). Even distribution ensures that the centrifuge...Using the centrifuge in an unbalanced state can damage the centrifuge and be dangerous for the user. If...different protocols. Improper use of a centrifuge can damage the equipment, your samples, or you, so be sure...
Showing: 181 - 200 of 470 results