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We narrowed to 660 results for: otos

Showing: 181 - 200 of 660 results
  1. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

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    Blog Post
    ... of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus with ...methods to use CRISPR to detect SARS-CoV-2. Other protocols and tools for detection have been made openly ... Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents for Pseudotyping Lentiviral Particles...
  2. High Complexity Golden Gate Assembly with NEB’s DAD

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    Blog Post
    ...the Type IIS restriction enzyme and thermocycler protocol. It will also identify overhangs with a high potential...possible, NEB also optimized the thermocycler protocols and made sure that T4 ligase was the correct choice...screening for selection, failed under normal cycling protocols, and required a 48-hour incubation at 37 °C. While...
  3. Viral Vectors 101: Virus Safety

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    Blog Post
    ...viruses also have a propensity to integrate into proto-oncogenes, which can lead to cancer. These viruses...reason for lab accidents is not following safety protocols.) If administering virus to mice, part of your... be safe is to know and closely follow safety protocols. This is especially true when you’re tired, in...
  4. Tips for Titering Your Lentiviral Preps

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    Blog Post
    ...drawbacks of different methods (for comprehensive protocols for all of the methods discussed here refer to...staining. Check Out Our In-Depth Fluoresence Titering Protocol Another popular approach utilizes the antibiotic...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 2....
  5. Troubleshooting and Optimizing a Western Blot

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    Blog Post
    ...Sonication based lysing protocol Subcellular fractional kit or lysing protocol Not enough target...using a subcellular fractionation kit or a lysing protocol developed for isolating proteins from a specific...errors Search online/ask an expert Review protocol videos for a visual guide to good technique ...
  6. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    Blog Post
    ...genome, removing the risk of oncogenicity or genotoxicity seen with other vectors (Lukashev et al., 2016...along with a description of their experimental protocol. The three plasmids which have been deposited ...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK....
  7. Cloning Mammalian Cells with the Agarose Method

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    Blog Post
    ...figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning method procedure...Vectors Resources on Addgene.org Check Out Our Protocols Pages Browse Fluorescent Protein Plasmids Find...
  8. PCR Applications for Copy Number Variation Assays

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    Blog Post
    ...Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer  ...variants based on real-time LightCycler PCR. Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014...
  9. Viral Vectors 101: Producing Your rAAV

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    Blog Post
    ... process, check out the Addgene AAV Production protocol. Titering Once you’ve produced, harvested, and...process, refer to the Addgene AAV Titration by qPCR Protocol. If you’re interested in the ddPCR titering process...Transfer PlasmidAAV Variables That Matter AAV protocols on addgene.org  AAV Titration by qPCR Using SYBR...
  10. Genome-wide Screening Using CRISPR

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    Blog Post
    ...of the genome and located immediately 5’ to a protospacer adjacent motif (PAM) sequence. Co-delivery of...a given phenotype. Image Credit: www.flickr.com/photos/72005145@N00/5600978712/ What CRISPR-based ...Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology 115: . https://doi.org/10.1002...
  11. Transferable Skills Guide: Identifying Your Transferable Skills

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    Blog Post
    ...you’ve gained experience working with similar protocols, and look - they’re seeking someone who has, to...Maybe there was a time you noticed an issue with a protocol and you took it upon yourself to fix the issue...you are in charge of completing many meticulous protocols or tasks, you’ve probably developed an attention...
  12. Summer SciComm Series: Modes of Communication

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    Blog Post
    ...and ecology can make for beautifully compelling photos and videos, while molecular, micro, and cell biology...academic to informal writing can difficult.  Photography Medium (high for microscopy) Visually compelling...context via still images. Some things cannot be photographed or need text to properly contextualize. Graphics...
  13. Droplet Digital PCR for AAV Quantitation

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    Blog Post
    ...Learn our protocol for AAV titration by qPCR Browse our collection of viral vector related protocols Learn...Browse our blog posts related to viral vector protocols and tips Read all of our viral vectors blog posts...
  14. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

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    Blog Post
    ... target is present immediately upstream of a Protospacer Adjacent Motif (PAM), the sequence NGG.  If these... within the editing window (bases 4-8 of the protospacer). While this is a small window, this system can... target sequence has been modified!  The exact protocol may vary depending on your specific experiment...
  15. QC Sequencing Technologies at Addgene

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    Blog Post
    ...them. DNA or RNA is pulled through the pore via motor proteins. When each nucleotide passes through the...Next‐Generation sequencing Technologies. Current Protocols in Molecular Biology, 122(1). https://doi.org/... Molecular Biology Reference Sequence Analyzer Protocol: Sequence Analysis of a Plasmid ...
Showing: 181 - 200 of 660 results