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We narrowed to 658 results for: otos

Showing: 181 - 200 of 658 results

  1. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    ...combine computational processing with biological protocols and revolutionary physical approaches. However...the structure in a similar way to the biological protocol, and assembly designs based around the use of ...commercially available tools and components. Rapid prototyping techniques such as 3D printing, on-demand CNC...

  2. Plasmids for Stem Cell Research

    Type
    Collection
    ...activators. Nat Commun. 2018 Jul 6;9(1):2643. Otonkoski Lentivirus Human Expression of human Sox2, Nanog... doi: 10.1038/nature10323. Crabtree Fibroblasts Motor Neurons Retroviral Mouse/Human Conversion of mouse...mouse and human fibroblasts into functional spinal motor neurons. Cell Stem Cell. 2011 Sep 2;9(3):205-18....2015 Nov 5;17(5):543-56. Buganim Skin Fibroblasts Motor Neurons Lentiviral Human Direct Lineage Reprogramming...Reprogramming Reveals Disease-Specific Phenotypes of Motor Neurons from Human ALS Patients. Cell Rep. 2016 Jan...2023(15)30159-6. Brennand iPSCs Cortical or Lower Motor Neurons CRISPR/TALEN Human Transcription Factor-...Differentiation of Human iPSCs into Neurons. Curr Protoc Cell Biol. 2018 Jun;79(1):e51. Next generation ...

  3. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type
    Blog Post
    ...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in...2018 and involved presentations and a handout on protocol sharing, material sharing, computational reproducibility...management plans, electronic lab notebooks, wet lab protocols, reagent sharing, bioinformatic tools, data and...

  4. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

    Type
    Blog Post
    ...selecting a bright fluorescent protein and a photostable fluorescent protein. In the third post of this...applications (e.g. labeling of cells or tracking promotor activity), but it is problematic in applications...verify a number of key properties (brightness, photostability, oligomerization) of a number of promising ...

  5. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    ... area. Unless you are looking for a particular protocol or are trying to replicate an experiment, the ...to the methods section for finer points of the protocols. Are there any red flags about how the work was...Louisiana State University. She is a fan of photosynthesis and writes about autotrophs and biochemistry...

  8. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

    Type
    Blog Post
    ...CRISPR-Cas activity. For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity... blue light (Bubeck et al., 2018). 3. Reduce cytotoxicity of CRISPR-Cas editing in tissues The small size... systems prevents the off-target effects and cytotoxicity that result from excessive nuclease activity...

  9. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    ... and delivers it cargo to certain cell types.  Botond Roska’s lab at the Institute of Molecular and Clinical...types (ex: rods or cones) or cell classes (ex: photoreceptors which encompassed both rods and cones).  Targeted...also expressed. If Cre-mCherry expresses in photoreceptors and horizontal cells, and Cre-dependent-GFP...

  10. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ... of a BSL-3 lab. Jesse Bloom’s lab published a protocol and reagents for pseudotyping lentivirus with ...methods to use CRISPR to detect SARS-CoV-2. Other protocols and tools for detection have been made openly ... Pettie D, King NP, Balazs AB, Bloom JD (2020) Protocol and Reagents for Pseudotyping Lentiviral Particles...

  11. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...the Type IIS restriction enzyme and thermocycler protocol. It will also identify overhangs with a high potential...possible, NEB also optimized the thermocycler protocols and made sure that T4 ligase was the correct choice...screening for selection, failed under normal cycling protocols, and required a 48-hour incubation at 37 °C. While...

  12. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...viruses also have a propensity to integrate into proto-oncogenes, which can lead to cancer. These viruses...reason for lab accidents is not following safety protocols.) If administering virus to mice, part of your... be safe is to know and closely follow safety protocols. This is especially true when you’re tired, in...

  14. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...drawbacks of different methods (for comprehensive protocols for all of the methods discussed here refer to...staining. Check Out Our In-Depth Fluoresence Titering Protocol Another popular approach utilizes the antibiotic...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 2....

  15. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...Sonication based lysing protocol Subcellular fractional kit or lysing protocol Not enough target...using a subcellular fractionation kit or a lysing protocol developed for isolating proteins from a specific...errors Search online/ask an expert Review protocol videos for a visual guide to good technique ...

  17. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...figure 1.   Find Additional Useful Protocols on Addgene's Protocol Pages Agarose cloning method procedure...Vectors Resources on Addgene.org Check Out Our Protocols Pages Browse Fluorescent Protein Plasmids Find...

  18. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    ...genome, removing the risk of oncogenicity or genotoxicity seen with other vectors (Lukashev et al., 2016...along with a description of their experimental protocol. The three plasmids which have been deposited ...Gonçalves and Ventura labs’ plasmids, including protocols, check out the plasmids at Addgene: pAdSh.PGK....

  19. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer  ...variants based on real-time LightCycler PCR. Curr Protoc Hum Genet. 2014;80:7.21.1-7.21.. Published 2014...

  20. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ... process, check out the Addgene AAV Production protocol. Titering Once you’ve produced, harvested, and...process, refer to the Addgene AAV Titration by qPCR Protocol. If you’re interested in the ddPCR titering process...Transfer PlasmidAAV Variables That Matter AAV protocols on addgene.org  AAV Titration by qPCR Using SYBR...
Showing: 181 - 200 of 658 results