We narrowed to 303 results for: rigi

1. Multiplex Genome Editing with CRISPR-Cpf1

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   ...crRNA array (bolded red arrow).  This undoes the original reversing of the direct repeats, leading to expression...

2. Screening for Successful Genome Editing with Digital PCR

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   ... number of target DNA molecules present in the original sample with a great deal of precision. This means...

3. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   ...much higher base editing activity compared to the original TadA-7.10 enzyme. After testing numerous TadA ...

4. Developing a cold tolerant E. coli using specialized chaperones

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   ...a much slower rate under cold temperatures, and frigid conditions decrease the potential for microbial...

5. Targeted Mutagenesis with EvolvR

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   ...targeted mutagenesis rate ~9-fold higher than the original nCas9, while increasing the standard mutation ...

6. 15 Years of Addgene: The Top 15 Plasmids

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   ...Note: this plasmid was called pLKO-Tet-On in the original publication but its name was subsequently changed...

7. Viral Vectors 101: Inverted Terminal Repeats

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   ...plasmid.  Within the AAV genome, ITRs serve as the origin of replication and are required for replication...

8. Viral Vectors 101: Viral Vector Elements

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   ...cells. In adenovirus, the ITRs serve as a type of origin of replication to initiate replication of the dsDNA...

9. Viral Vectors 101: The AAV Retrograde Serotype

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   ... forth and trace some neurons!  This post was originally written by Leila Haery in 2017 and significantly...

10. How Addgene Creates Content

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    Blog Post

    ...scientific support staff receives – in fact, the blog originally started as a way to easily store and share in-depth...

11. Antibodies 101: Affinity Reagents

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    ...the orientation of the two fragments. However, rigidity can be introduced through mutation, allowing diabodies...

12. RNA Extraction Without A Kit

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    ...homogenization solution and phenol addition of the original no-kit protocol into one step. After homogenization...

13. Visualizing Protein Turnover In Situ

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    ... clever combination of FUNCAT and PLA PLA was originally published by the Söderberg lab in Sweden as a...

14. The time and cost required to make a plasmid

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    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...

15. CRISPR 101: Cas9 vs. The Other Cas(s)

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    ... deletions.   Cas12a – The staggered cutter  Originally classified as Cpf1, Cas12’s primary difference...

16. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

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    ...inconsistencies across geographical regions When we originally started gathering this information, many assumed...

17. New Tool for Lineage Tracing: The ClonTracer Library

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    ...drug discovery process.” The ClonTracer library originally came about as Bhang and co-workers were trying...

18. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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    ...was contributed by Johnna Roose. This post was originally published on Johnna's New Under The Sun Blog ...

19. Working with Nuclear Receptors

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    ... through factors (both of endocrine and dietary origin) that are diffused or actively transported from...

20. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ...dCas9. Within a few rounds of cell division, the original methylated strand is diluted to undetectable amounts...