...need accessory packaging vectors to generate the virus. Bacterial vectors This system is great for overexpressing...

...Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids ...

...vector packaging service, but lets us know what viruses would be helpful to the scientific community....

...'ve ignored those used in eukaryotic cells and viruses and focused only on those found in bacteria. Some...

...does not raise the safety concerns associated with virus work. Fourth, working directly with proteins removes...

...and minimize misfolding and recombination during virus production needed to deliver CRISPR-Sirius into ...

...Central PMCID: PMC5918177. Volak, Adrienn, et al. "Virus vector-mediated genetic modification of brain tumor...

...elements for deliveries via adeno-associated virus (AAV) and lentivirus, demonstrating simultaneous editing of...

...These new iGluSnFR3 variants come in AAV packaged virus aliquots being deposited at Addgene. Other plasmids...

...replication. Cell lines expressing the Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T...

...delivery methods include retrovirus [2], lentivirus [4], adenovirus [5], Sendai virus [6], plasmid electroporation...stem cells (iPSCs) by temperature-sensitive Sendai virus vectors. Proc Natl Acad Sci U S A, 2011. 108(34)...

...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Return...

... a gene (cDNA) can be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for...mechanism to integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery...

... lab suspected that the co-transduction by two viruses would lower editing efficiency, the split editors...

...share information about the emerging SARS-CoV-2 virus quickly and openly. By sharing results faster and...

...elements, to examine their function. Bacteria and viruses have illustrated the power of polycistronic gene...

...simply fused the wild-type Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase to the C-terminus ...was made possible through their use of engineered virus-like particles. However, there’s still a long way...

...library to screen for genes involved in West Nile Virus (WNV) induced cell death. The Wu lab library consists.... PubMed PMID: 26837755   SEPTEMBER 2016 Zika virus plasmids Now avaliable through Addgene The Vaithi... Addgene’s repository. The genes, from the Zika virus Asian genotype PRVABC59, were obtained from the ...using direct transfection precluding the need for virus. Furthermore, dCAS9-VPR expression is under the ...First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors...use the library, the authors packaged it into lentivirus and used the resulting lentiviral library to ...dCas9-VPR limits one’s ability to package it into lentivirus and later create stable cell lines expressing...

...tested in rats. Abbreviations: AAV, adeno-associated virus; GRE, gene regulatory element; NHP, non-human primate...vector packaging service, but lets us know what viruses would be helpful to the scientific community. Text...

... the virus.  Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from... options for Cpf1 multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA... multiplex system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the DNA sequence...