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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 323 results for: virus

Showing: 181 - 200 of 323 results

  1. Deisseroth INTRSECT Collection

    Type
    Collection
    ...targeting strategy that allows adeno-associated virus (AAV)-borne payloads to be expressed in cells based...recombinase-expressing animal lines and retro-grade expressing viruses delivering additional recombinases 10–14 . Figure...principles for working with INTRSECT recombinases, viruses, and important controls to consider as part of ...

  3. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...does not raise the safety concerns associated with virus work. Fourth, working directly with proteins removes...

  4. Plasmid Collections

    Type
    Collection
    ...Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus All Viral Plasmids ...

  9. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...replication. Cell lines expressing the Epstein–Barr virus (EBV) nuclear antigen 1 (EBNA1) or the SV40 large-T...

  11. Plasmids 101: The protein expression toolbox

    Type
    Blog Post
    ... a gene (cDNA) can be done by either plasmid or virus. A plasmid carrying your cDNA can be targeted for...mechanism to integrate your choice cDNA. Both lentivirus and retrovirus can package cDNAs for genomic delivery...

  16. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...library to screen for genes involved in West Nile Virus (WNV) induced cell death. The Wu lab library consists.... PubMed PMID: 26837755   SEPTEMBER 2016 Zika virus plasmids Now avaliable through Addgene The Vaithi... Addgene’s repository. The genes, from the Zika virus Asian genotype PRVABC59, were obtained from the ...using direct transfection precluding the need for virus. Furthermore, dCAS9-VPR expression is under the ...First, GMAP-compatible backbones for lentivirus LV 1-5 and retrovirus RV 2-5 were constructed. The authors...use the library, the authors packaged it into lentivirus and used the resulting lentiviral library to ...dCas9-VPR limits one’s ability to package it into lentivirus and later create stable cell lines expressing...

  19. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ... the virus.  Reversing the orientation of the direct repeat protects the (+) stranded lentivirus RNA from... options for Cpf1 multiplexing: Transfection, lentivirus, and AAV Cpf1’s ability to process its own pre-crRNA... multiplex system from a lentiviral vector.  Lentiviruses carry a (+) strand RNA copy of the DNA sequence...

  20. To Each HIS Own

    Type
    Blog Post
    ...histidine-tagged protein expressed by recombinant vaccinia virus. Proc Natl Acad Sci U S A. 1991;88(20):8972-6. Moks...
Showing: 181 - 200 of 323 results