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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 110 results for: PIP

Showing: 101 - 110 of 110 results

  1. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...minimizes the number of small volumes you need to pipet. If you are using the same template DNA for all ...wish to perform and add 10% extra to account for pipetting error. In this example, we have 7 different reactions... (0.14µl) = 1.54µl Mix the contents by gently pipetting up and down several times. Keep tube on ice. Add...mix so you know that the primers have been added, pipet the forward primer onto one side of the tube wall...

  2. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...chromosomes. Collect the supernatant into a new tube by pipetting or carefully pouring. Optional: Add 5 μL of 2 ...particles Bottom phase - Organic phase (protein) Pipet the aqueous DNA layer and place it in a new microfuge...the tube after the 70% ethanol wash. You can also pipet the supernatant out of the tube if you are worried...

  3. Pouring LB Agar Plates

    Type
    Protocol
    ... process works. Equipment Autoclave Water bath Pipetman Reagents 37 g pre-mixed powder consisting of: ...10 - 15 mL of agar. Autoclavable flasks Sterile pipettes Ice bucket to hold antibiotic Antibiotic at 1000...pouring. Measure your desired amount of agar with a pipete for the first plate to get a good idea of what ...

  4. Protocol - How to Streak a Plate

    Type
    Protocol
    ...stab or glycerol stock . Using a sterile loop, pipette tip or toothpick, touch the bacteria growing within...plate, to create streak #2. Using a third sterile pipette tip, toothpick, or sterilized loop, drag through...

  7. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ... formulation buffer and sample and make sure to pipet back and forth a few times to mix the iodixanol ...buffer. Use a P1000 to the bottom of the filter and pipette up/down and wash off the walls of the filter to...
Showing: 101 - 110 of 110 results