We narrowed to 113 results for: PIP

...Mulitchannel Pipetting Technique Learn how to pipette multiple samples at once using a multichannel pipette and...and avoid inconsistent pipetting with our simple tips Bacterial Transformations We walk you through a standard...

...use Watch the Video! Pipetting Learn about selecting the correct pipettor and pipette tip, how to dispense...dispense liquids, and how to handle the pipette Watch the Video! Centrifugation Learn about selecting and ...

...digest results. Equipment Electrophoresis chamber Pipetman Reagents Liquid DNA aliquot of your plasmid of...instructions) Gel loading dye Electrophoresis buffer Pipet tips Procedure Select restriction enzymes to digest....2-0.5 µL of enzyme because it is difficult to pipette less volume than this; 0.2-0.5 µL will likely be... more enzyme is usually better. Mix gently by pipetting. Incubate tube at appropriate temperature (usually...

...minimizes the number of small volumes you need to pipet. If you are using the same template DNA for all ...wish to perform and add 10% extra to account for pipetting error. In this example, we have 7 different reactions... (0.14µl) = 1.54µl Mix the contents by gently pipetting up and down several times. Keep tube on ice. Add...mix so you know that the primers have been added, pipet the forward primer onto one side of the tube wall...

...chromosomes. Collect the supernatant into a new tube by pipetting or carefully pouring. Optional: Add 5 μL of 2 ...particles Bottom phase - Organic phase (protein) Pipet the aqueous DNA layer and place it in a new microfuge...the tube after the 70% ethanol wash. You can also pipet the supernatant out of the tube if you are worried...

... process works. Equipment Autoclave Water bath Pipetman Reagents 37 g pre-mixed powder consisting of: ...10 - 15 mL of agar. Autoclavable flasks Sterile pipettes Ice bucket to hold antibiotic Antibiotic at 1000...pouring. Measure your desired amount of agar with a pipete for the first plate to get a good idea of what ...

...stab or glycerol stock . Using a sterile loop, pipette tip or toothpick, touch the bacteria growing within...plate, to create streak #2. Using a third sterile pipette tip, toothpick, or sterilized loop, drag through...

...restriction digest. Equipment Electrophoresis chamber Pipetman Pipet tips Reagents Liquid DNA aliquot of your plasmid...

...autoclave. Only mechanical pipetting should be done in the laboratory. Don’t mouth pipette! There may be some ...

... formulation buffer and sample and make sure to pipet back and forth a few times to mix the iodixanol ...buffer. Use a P1000 to the bottom of the filter and pipette up/down and wash off the walls of the filter to...