...a zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer...sites. (B) A TAL effector nuclease (TALEN). Similar in principle to the ZF nuclease, the components of the...the array recognize individual nucleotides. For over ten years, zinc finger (ZF) arrays have been the go-to...number of zinc fingers that recognize various nucleotide triplets. With some trial and error, ZF arrays...years of research has shown that when fused to a nuclease or activation domain, a ZF array is an effective...without their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and ...Bogdanove at Iowa State University published the nucleotide recognition code of the TAL effectors, which ...

...innate immune system; and reconfiguring of the nuclear compartment, gene expression, and protein translation...Adenovirus Tales: From the Cell Surface to the Nuclear Pore Complex. PLoS Pathog, 11(6), e1004821. https...

..., W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid of the Bryan strain of Rous sarcoma virus:...purification of the virus and isolation of the nucleic acid. Proceedings of the National Academy of Sciences...

...VchINT does not recruit other nucleases (such as Cas3) or have its own nuclease activity. Instead, it forms...

...antimicrobials using efficiently-delivered RNA-guided nucleases.” Nature Biotechnol. 32(11): 1141-5. (2014). PubMed... Bikard, David, et al. “Exploiting CRISPR-Cas nucleases to produce sequence-specific antimicrobials.” ...

...translocation trap (ITT) system to analyze signal-induced nuclear translocation of proteins in 2004. In addition,...addition, we have recently developed the oligoribonucleotide (ORN) interference-PCR (ORNi-PCR) method to suppress...

...10aa-H2B-WPRE3-BGHpA AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq ...pAAV-hDlx-GiDREADD-dTomato-Fishell-5 Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...pAAV-hDlx-GqDREADD-dTomato-Fishell-4 Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons...

...Previous research has shown that regions such as the nucleus accumbens (NAc), which is linked to the reward ...cytokine interleukin IL-1 beta in the dorsal raphe nucleus (DRN), a region thought to be involved in these...

...been demonstrated in zebrafish using zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another...deactivation method in zebrafish are short, modified oligonucleotides called morpholinos. When injected into zygotes...

...take the nucleus and put it in a fertilized egg to get edited embryos (replacing the nucleus is still ...

...profiling of off-target cleavage by CRISPR-Cas nucleases." Nature biotechnology 33.2 (2015): 187. Pubmed.... 3. Fu, Yanfang, et al. "Improving CRISPR-Cas nuclease specificity using truncated guide RNAs." Nature...

... base editors probably due to the presence of nucleosome or other proteins. You can detect base editing...PMC4873371.  2. Nishida, Keiji, et al. "Targeted nucleotide editing using hybrid prokaryotic and vertebrate...

...that MECP2 binds to methylated CpG dinucleotides and CAC trinucleotides during neuronal maturation (Tillotson...

...unfolding and sliding at upstream standby sites." Nucleic acids research42.4 (2013): 2646-2659. PubMed PMID...selection systems for unmodified Enterobacteriaceae." Nucleic acids research 43.13 (2015): e83-e83. PubMed PMID...

...fluorescence. In Brainbow 2.1, GFP is tagged with a nuclear localization sequence, allowing it to localize ...mouse generates cells that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic...

...results in a polymerase error rate of up to 2% per nucleotide position. The “sloppy” PCR reaction is created...degraded by uracil DNA glycosylase (UDG) and Exonuclease III (ExoIII) (step 4). UDG recognizes uracils...

... of certain expressed proteins endA, endA1 Endonuclease I (nonspecific cleavage of dsDNA ) mutation ...recombination, increases plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability...

...genome engineering technologies like zinc finger nucleases (ZFNs) and TALENs. CRISPR: An Adaptive Immune ...Through sequence homology, these crRNAs guide a Cas nuclease to the specified exogenous genetic material, which...the individual crRNAs. (3) The tracrRNA and Cas9 nuclease form a complex with each individual, unique crRNA... alternative PAM sites . Cpf1 , another CRISPR nuclease, recognizes a 5’-TTN PAM, improving targeting ...Cascio VM, Joung JK. 2014. Improving CRISPR-Cas nuclease specificity using truncated guide RNAs. Nat Biotechnol...Shinagawa H, Makino K, Amemura M, Nakata A. 1987. Nucleotide sequence of the iap gene, responsible for alkaline... E. 2012. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science . 337...

... CRISPR-mediated nucleic acid cleavage. We have harnessed the type II CRISPR nuclease system to facilitate...nterspaced S hort P alindromic R epeats) is a microbial nuclease system involved in defense against invading phages...by co-expressing the S. pyogenes Cas9 (SpCas9) nuclease along with the guide RNA. For most applications... have found that adding an additional guanine nucleotide to the 5’ of 20-bp guide can increase targeting... genes. It consists of three components: A nucleolytically inactive Cas9-VP64 fusion A sgRNA incorporating... have found that adding an additional guanine nucleotide to the 5’ of 21-bp guide can increase targeting...GFP-KASH fusion facilitates FACS sorting of cells and nuclei. Return to top AAV - Cas9 mouse CRISPR-Cas9 is ...

...include a marker like the Hoechst stain for the nuclei. (See Brewer et al, 2022 for a sample protocol.... (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels C1-C3 show magnified detail...