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We narrowed to 545 results for: SEC

Showing: 201 - 220 of 545 results

  1. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...usually added to the media for uptake by the cells. Second, you’ll need tRNAs and tRNA synthetases specific...incorporated into the cells via plasmid. Third, a second plasmid will be needed to introduce the gene of...

  3. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...meaning it is typically used in conjunction with a second vector that delivers gRNA, such as lentiGuide-Puro...useful tips for generating your own lentivirus. Secondly, you can use a LentiCas9-Blast viral prep to create...

  4. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ... the lab Scientists discovered that deleting a section from the lacZ gene (a mutation called lacZΔM15)...β-galactosidase enzyme. Providing DNA encoding this section of amino acids (called the α-peptide) to a lacZΔM15...

  5. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ...cells, hijack host cell machinery, and synthesize a second strand of DNA before expression of their cargo ...DNA goes through the inter-molecular annealing or second strand synthesis. In contrast, a scAAV vector with...

  9. Developing Lab Management Software for Biology

    Type
    Blog Post
    ... if there is either success or failure: In our second example we’ve added some simple but critical logic...procedures, not artificially constraining them). In the second example we have a separate worm model and tube ...

  11. Career Coaching for Scientists: Why and Where Do I Find One?

    Type
    Blog Post
    ... as effective in mentoring for this transition. Second, a coach will help you set goals and then hold ...us) offers a free report on her website called “Secrets to Success in Graduate School”. She has also launched...

  12. Working with Nuclear Receptors

    Type
    Blog Post
    ...with NRs) exist in steady-state complexes with secondary coregulator (or co-coregulator) partners. The ...information can be found in the Datasets and Reagents sections of the current NURSA website. NURSA and Addgene...

  13. Hot Plasmids February 2024

    Type
    Blog Post
    ... piece on mStayGold inspired and informed this section.  Find mStayGold plasmids at Addgene here!   Ando...  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with brilliance By Hridaya Patel...

  14. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...cargo-specific safety   After replication competency, the second concern with virus safety is the disease-risk of...ultracentrifugation, heat sealed tubes can be used for extra security. Finally, when spinning down lentivirus, you’ll...

  17. Lab Automation at Addgene

    Type
    Blog Post
    ...easy sample transfer between 96 well plates. The second pod can pick up 8 tips and position the tips indepently... tricks, be sure to share them in the comments section.   Additional Resources on the Addgene Blog Apps...

  18. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...of the new viral preps from recent months: New INTRSECT viral preps available!  We are expanding our inventory...ready to use viral preps from the Deisseroth lab INTRSECT project. Check out the new additions.  Image calcium...

  19. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...and mVenus (bottom). Cells were imaged every 30 seconds for 3 hours. Image from Lee et al., 2020. SPOTlight....A184S-AAV1 here New Dual and Triple feature INTRSECT viral preps from the Deisseroth Lab now available...

  20. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...one end of the DNA fragment (Figure 1, middle). A second recombination event then excises the DNA between...invert the reporter and GeneR fragment. Then the second recombination event would excise the reporter from...
Showing: 201 - 220 of 545 results