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We narrowed to 643 results for: ato

Showing: 301 - 330 of 643 results

  1. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    ...our curated list of biosensors too, as another laboratory may have already created the sensor that you ...PMC18018. 2. Miyawaki, Atsushi, et al. "Fluorescent indicators for Ca2+ based on green fluorescent proteins ...

  2. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

    Type
    Blog Post
    ...zebrafish), D. melanogaster (fly), C. elegans (nematode), S. cerevisiae (budding yeast), S. pombe (fission...Distribute pending QC. The name of the Principal Investigator and Organization where your plasmids were first...

  3. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...high-quality antibodies for IHC. Working with collaborators and support from the Brain Research Through ...intuitive guide for checking their staining and neuroanatomy without the fear of referencing a wrong section...

  6. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...human tRNA genes and, until now, we've overlooked regulatory fragments of these transcripts. I remember when...Medicine presented another unexpected finding in the regulatory RNA field. The Rajewsky lab is studying circular...

  8. Pooled Library Amplifications

    Type
    Blog Post
    ...protocol, the size of the cuvettes your lab’s electroporator uses, etc. The DNA amount can be anywhere from...Screening Zhang Lab - Multiplexed Overexpression of Regulatory Factors (MORF) Library 3,548 250 - 10,000...

  9. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...If you have ever worked in a molecular biology laboratory you have likely done a polymerase chain reaction...method that can help save time and effort in the laboratory.  There are two main categories of multiplex ...

  10. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...additional protein characteristics. For example, Masato Kanemaki's lab has developed a CRISPR/Cas-based...of auxin to the culture medium. Any number of regulatory sequences, including protein enhancers and repressors...

  11. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ...in real time! I follow great online science communicators like Phil Plait @BadAstronomer, Emily Graslie...when I was trying to find local informal science educators, and ever since, the State Museum has been the...

  12. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    ...viral vectors) without triggering a destructive inflammatory immune response. At the time, adenoviral vectors...choroideremia, X-linked retinitis pigmentosa, achromatopsia, and age-related macular degeneration are ongoing...

  16. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ... confident that their teammates and project collaborators have completed their work thoroughly. It’s kind...Veckerelli is the Administrative and Operations Coordinator at Addgene. In addition to her operational responsibilities...

  18. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ... region is transferred into the plant. In the laboratory we can utilize this natural process by replacing...and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration can often be seen by...

  19. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...famous ones are influenza hemagglutinin (HA), myelocytomatosis viral oncogene (myc), simian virus 5-derived...usually provided by red fluorescent proteins such as tdTomato or RFP or blue fluorescent proteins like Cyan....

  20. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    ...fluorophore protein. Years later, Samie Jaffrey’s laboratory took advantage of their deep knowledge of the...Farzin. RNA Nanotechnology and Therapeutics. Boca Raton: CRC Press, 2014. 5. Hayat, Akhtar and Marty, Jean...

  21. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...available in the repository. GCaMP, a calcium indicator (biosensor, by Addgene’s categorization) was first...Targeted Neuronal Manipulation. Frontiers in Neuroanatomy, 13. https://www.frontiersin.org/articles/10.3389...

  22. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...carefully regulated by a mix of positive and negative regulators. The genome of E. coli is about 4.6 million ...trading out ADP for ATP to re-activate DnaA. These regulatory mechanisms allow E. coli to control chromosomal...

  23. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    ...different bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number...own replication machinery such as Rep or other initiator proteins, they do not require host proteins for...

  25. Hot Plasmids - November 2023

    Type
    Blog Post
    ...example, neurons treated with a phorbol ester activator gave fluorescent signal in the ticker tape from...transmission, and plasticity and is a popular marker of excitatory synapses in assays like IHC. In fact, Anti-PSD...

  26. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...more feasible, the lab of Alessio Ciulli and collaborators developed CRBNmidi (Kroupova et al., 2024). ...Bentley Time to get flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS...

  27. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...resistant infection or not, because scientists in the laboratory will need to isolate and grow whatever bacteria...method that is frequently used in the clinical laboratory. What can we do to stop the emergence of antibiotic...

  28. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...studies on reducing antibiotic prescriptions for respiratory tract infections. The video abstracts provide...  “Singing” Fish Rely on Circadian Rhythm and Melatonin for the Timing of Nocturnal Courtship Vocalization...
Showing: 301 - 330 of 643 results