We narrowed to 386 results for: nans

1. Mouse Modeling, Part 1: Genetically Engineered Mice

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   ...Outbred strains of mice are useful for comparing dominant and recessive traits and their impact on diseases...

2. A Look at Addgene's QC Process

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   ...plasmids are sent out to Plasmidsaurus for long-read nanopore sequencing. This platform sequences the entire...

3. Design Tips for Prime Editing

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   ...prime editing systems by manipulating cellular determinants of editing outcomes. Cell, 184(22), 5635-5652...

4. Viral Vectors 101: Chemogenetics

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   ...Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D, Kullmann DM, Lieb A (2019...

5. Plasmids 101: FLEx Vectors

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   ...Saunders A, Johnson CA, Sabatini BL. 2012. Novel recombinant adeno-associated viruses for Cre activated and...

6. Plasmids 101: Terminators and PolyA signals

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   ...many studies focus on promoter strength as a determinant of gene expression levels, the terminator also...

7. Experimenting in an Open Source lab: from CRISPR to Cats

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   ... a new project at a makerspace is finding the financial resources to pursue it. Since makerspaces are ...

8. Plasmids 101: 5 factors to help you choose the right cloning method

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   ... to the use of toxic ccdB to prevent any non-recombinant vectors from propagating. The ligation-independent...

9. Fluorescent Tagging of Endogenous Genes with SapTrap

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   ...plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP...

10. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    ...feature of balancer chromosomes is that they produce dominant phenotypes visible on the flies. For example, ...

11. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    ...between two molecules more closely is Förster Resonance Energy Transfer, FRET. Additional opportunities...

12. Truncated gRNAs for Regulating Gene Expression

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    ...where her research is focused on engineering nanoparticles as drug delivery systems. She is also a NASA...

13. Pooled Library Amplifications

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    ...For larger libraries that require hundreds of nanograms of DNA, you will likely need to perform several...

14. Production of Virus in Insect Versus Mammalian Cells

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    ...). HEK293 cell line as a platform to produce recombinant proteins and viral vectors. Frontiers in Bioengineering...

15. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    ...Antibody GuideAddgene's collection of ready-to-use recombinant antibodies...

16. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

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    ...fluorescence lifetime imaging microscopy-based Förster resonance energy transfer (FLIM-FRET) in living cells (Figure...

17. Plasmids 101: Knockout/Knock-In Plasmids

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    ...marker HSV-tk is used to select against random recombinants.   Design your targeting construct. For...

18. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    ...particular type will interact. A protein with a nanomolar Kd will exist primarily as a dimer in cells while...

19. PITChing MMEJ as an Alternative Route for Gene Editing

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    ...which has been difficult to modify due to NHEJ’s dominance over HDR in this species. As CRISPR technology...

20. Which Fluorescent Protein Should I Use?

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    ...effect called FRET (fluorescence [or Förster] resonance energy transfer). FRET occurs when energy transfer...

21. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...Medical Center.  You’ve probably heard of Forster Resonance Energy Transfer (FRET). Through the non-radiative...

22. Viral Vectors 101: The Retroviral Lifecycle

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    ...is present in the capsid, as the engineered, recombinant genome is (thankfully) gutted of many essential...

23. Droplet Digital PCR for AAV Quantitation

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    ...tool for titration and integrity evaluation of recombinant adeno-associated viral vectors." Human Gene Therapy...

24. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ...In this example we’re going to be looking for recombinant monoclonal antibody plasmids from the James Trimmer...

25. Plasmids 101: Colony PCR

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    ...Resources Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR ...

26. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

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    ... among the earliest uses of GFP. Fluorescence resonance energy transfer (FRET): This is used to study ...

27. Neuronal labeling with Spaghetti Monster

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    ...neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV) has been widely ...

28. How to Write a Scientific Review Article

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    ...deacetylases in B-and T-cell development, physiology and malignancy." Genes & cancer 6.5-6 (2015): 184. PubMed PMID...

29. Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More

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    ...three-month generation time and labor-intensive maintenance compared to invertebrate model systems, and their...

30. Save Time with Transient Plant Leaf Transformations

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    ...antibodies in plants using syringe agroinfiltration." Recombinant proteins from plants. Humana Press, 2009. 41-...