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Showing: 401 - 440 of 965 results
  1. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post
    ...the more detailed questions the organizers wanted to touch on in organizer David Riglar’s blog post. Essentially...Symposium On the FOR website, the FOR Boston 2015 organizers indicate that the main question they wanted to...panel was that there are many companies and organizations (including Addgene), that need people who are...
  2. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...in the comments or shoot us an e-mail at blog@addgene.org and maybe we can write a blog post about it! ...including subcellular visualization of 6 different organelles, a cell cycle indicator for mouse embryonic stem...mitochondrial fusion with a new tri-chromatic assay Georg Ramm’s lab at Monash University has developed a ...viability. In their recent work in Nature Methods, Georgieva et al describe the rational production of a new...have fewer effects when fused to your POI. Georgieva et al developed the inntags via a bioinformatics...information on inntags, read our recent blog post. Georgieva, et al. Nature Methods. 2015 Aug 31. CRISPR-Display...monitoring cargo trafficking, and for discovering organelle contact sites. This suite of deposited fluorescent...
  3. Plasmids 101: Repressible Promoters

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    Blog Post
    ...has also been implemented in a variety of other organisms, such as sphingomonads and bacillus.  ADH1 The...mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750-6-43Kaczmarczyk A, Vorholt JA...overexpression transgenes. Sci Rep 12, 3835 (2022). https://doi.org/10.1038/s41598-022-07852-7 De Boer, Herman A., ...and PolyA Signals FLEx Vectors Resources at Addgene.org Tetracycline Collection ...
  4. A Guide to Getting Started in Undergrad Research

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    Blog Post
    ...Psychological and Personality Science 11:647–657 . https://doi.org/10.1177/1948550619882032 Chrousos GP, Mentis A-...threatens diversity. Science 367:749.2-750 . https://doi.org/10.1126/science.aba8039 Cordova FA (2016) Embrace...Embrace uncertainty. Science 351:994–994 . https://doi.org/10.1126/science.351.6276.994 Moore E (2021) One...I built a network of mentors. Science. https://doi.org/10.1126/science.caredit.abi6939 Additional resources...
  5. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    Blog Post
    ...Cell Culture. Anal Chem 84:4227–4232 . https://doi.org/10.1021/ac2033112 Ligasová A, Vydržalová M, Buriánová...Fluorescence Microscopy. Cells 8:1510 . https://doi.org/10.3390/cells8121510 Sung H, Kang SH, Bae YJ, Hong...Lines. In Vitro Cell Dev Biol Anim 38:86 . https://doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2...for working with viral vectors Resources on Addgene.org Check out Addgene's written and video protocols...
  6. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post
    ... blood stream to commute to and invade multiple organs, and most importantly escape the immune mechanisms...environments for tumor cells spreading to other organs during metastasis. Evading Recognition Tumor cells...2002): 991-998. PubMed PMID: 12407406. 4. Weiner, George J. "Building better monoclonal antibody-based therapeutics...Ghana Modulate Cancer Signalling Resources on Addgene.org Check out Our Cancer Collection Pages Use the...
  7. Antibodies 101: Flow Cytometry

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    Blog Post
    ...of Scientific Instruments 43:404–409 . https://doi.org/10.1063/1.1685647 McKinnon KM (2018) Flow Cytometry...Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot J, Guerin CL, Le Van Kim ...instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616-011-9415-0 Additional resources ...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...
  8. Purple Plants that Report Pollutants

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    Blog Post
    ...biology world, many of scientists’ favorite model organisms belong to a group called dicots — think Arabidopsis...-Gamboa, L., Saleh, L., Pathak, S., Swyers, N., Morgan, A., Meerdink, S., Kuzio, C., Calderon, S., Sheng... Grasses. Plant Biotechnology Journal. https://doi.org/10.1111/pbi.70480 Additional Resources Browse...
  9. Interview with Bart Newland, Addgene’s Newest Board Member

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    Blog Post
    ...of a lawyer can bring a new perspective to an organization. I'm used to thinking about the downstream results...how to manage them without them paralyzing an organization. I think these are things that I can bring to...that business experience and judgement to the organization and see how I can help. Q: What do you enjoy...
  10. Addgene’s Top 10 Blog Posts from 2014

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    Blog Post
    ...research with the community, so email us at blog@addgene.org if you are interested. Addgene's Most Popular...Plasmids 101: Origin of Replication By Kendall Morgan Save Time and Money by Making Your Own Competent...101: The Promoter Region – Let's Go! By Kendall Morgan Plasmids 101: Mammalian Vectors By Marcy Patrick...
  11. Let There Be LITE Plasmids

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    Blog Post
    ...tissue. “The brain is such an amazing and dynamic organ,” Konermann explained. “It helps us adapt to influences...use is by no means limited to particular model organisms or cell types. “This tool is really flexible and... Kendall Morgan...
  12. Modulate the Activity of 17 Signaling Pathways with One Kit!

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    Blog Post
    ...growth, survival, and differentiation.” https://www.addgene.org/kits/sabatini-wood-cancer-pathway-orfs/ Finding...Technologies from 2015 Additional Resources on Addgene.org Find Other Kits for Your Research Find Pooled... Kendall Morgan...
  13. Reflections and Looking Ahead

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    Blog Post
    .... Even more remarkably, 1,160 labs from 522 organizations in 41 countries enriched our repository with...antibody characterization. Nature Protocols. https://doi.org/10.1038/s41596-024-01095-8 Kahn, R. A., Virk, H...biomedical research. eLife, 13, e100211. https://doi.org/10.7554/eLife.100211...
  14. From our Table to Yours: An Inside Look at Lunch at Addgene

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    Blog Post
    ...sports, movies, and just about anything. As our organization grew, we continued this tradition. We now have...delicious) team building activity that is easy to organize. We’ll dedicate a post to these competitions in...Hack Days. During Hack Days, teams at Addgene will forgo their regular To-Do lists (other than emergency ...
  15. SciComm with the Experts at Science in the News Part 2

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    Blog Post
    ...Set up your own Science by the Pint series and organize evens where scientists can grab a beer and chat...institutional knowledge and continuity - two lead organizers on each program, one junior and one senior.  ...For more info about starting your own SciComm organization and to learn about some of SITN's other initiatives...
  16. Celebrating Accomplishments in the Lab

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    Blog Post
    ...lab do for fun as a group? The Ellis Lab self-organizes cinema nights, game nights, and trips to eat out...flexibility and you aren’t locked into a schedule.” George Church comments on how he would go home in the ... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) for taking the ...
  17. Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections

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    Blog Post
    ...collections pages, please shoot us an e-mail at help@addgene.org. We’d love to hear your feedback. Additional ...set of plasmids you’ve deposited with us. Don't forget to get yourself out there! Additional Resources...Inside Addgene Posts Additional Resources on Addgene.org Browse Our Special Collections Pages Suggest...
  18. IBEX Knowledge-Base: A Data Resource for Multiplex Tissue Imaging

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    Blog Post
    ...reagent type, target species, target name, host organism, isotype, clone details, vendor, method, tissue...tissues. Nature Protocols, 17(2), Article 2. https://doi.org/10.1038/s41596-021-00644-9 Radtke, A. J., Kandov...Academy of Sciences, 117(52), 33455–33465. https://doi.org/10.1073/pnas.2018488117 More resources on the Addgene...
  19. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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    Blog Post
    ... happy to have the support of the following organizations to share materials more broadly to accelerate...to industry? Over the past 8 years, several organizations have made their plasmids available beyond academia...to industry, please reach out at techtransfer@addgene.org and we will initiate a conversation with you ...
  20. 9 Tips to Achieve Success in Academia

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    Blog Post
    ... Connie Cepko (Harvard Medical School), and Dr. George Church (Harvard Medical School) who took the time...helps when the time comes to work with full force. George Church 1. “Initial and sustained motivation is ...Thank you again to Connie Cepko, Tom Ellis, and George Church for answering our burning questions about...
  21. In Living Color: The Skinny on In Vivo Imaging Tools

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    Blog Post
    ...peering right through living animals to see their organs – a liver or brain, say, or maybe a tumor. They...for optical labeling and tracking of proteins, organelles, and living cells in a spatiotemporal manner,...  Kendall Morgan...
  22. Tips for Improving Your Next Manuscript

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    Blog Post
    ...Searching, Reading, & Organizing Write for the Addgene Blog Resources on Addgene.org Check out Our Revamped...straight to the point. 3. Engage the reader Scientists forget that the reader is not in their head. The scientist...
  23. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    ... Disease that runs on behalf of the National Organization for Rare Disorders (NORD). NORD raises money...We will be collecting donations for IFOPA, an organization dedicated to raising funds and awareness of ...Potentiall HIV Treatments with CRIPSR Resources on Addgene.org Find ACVR1 Plasmids Find Plasmids for Disease...
  24. Addgene at the ASM Microbe 2016 Conference

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    Blog Post
    ...getting a badge illustrated with my favorite microorganism (Rhodobacter sphaeroides!) at the ASM booth....with the scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution...Network at Scientific Conferences Resources on Addgene.org Check out Our Microbiology Resource Page Deposit...
  25. Antibodies 101: Affinity Tags

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    Blog Post
    ...Humana Press. https://doi.org/10.1385/1-59259-301-1:99 Resources on Addgene.org Addgene antibodies ...Protein Science. John Wiley & Sons, Inc. https://doi.org/10.1002/0471140864 Fox, J. D., & Waugh, D. S. (...
  26. Antibodies 101: Fc Effector Functions

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    Blog Post
    ... responses. Cell Res 20, 34–50 (2010). https://doi.org/10.1038/cr.2009.139 Janda, A., Bowen, A., Greenspan...Function. Frontiers in Microbiology, 7. https://www.frontiersin.org/articles/10.3389/fmicb.2016.00022 Torres,...specificity. Trends in Immunology, 29(2), 91–97. https://doi.org/10.1016/j.it.2007.11.004 Xu Cao, et al. Promoting...
  27. Some Like it Hot: Thermostable GeoCas9

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    Blog Post
    ... editing tool of choice for a number of model organisms: mammalian cells, yeast, drosophila, plants, worms...obligate thermophiles without the need to grow the organisms at lower than optimal temperatures. GeoCas9’s ...Blog Posts on CRISPR Additional Resources on Addgene.org Brush Up on Your CRISPR Background with Addgene...
  28. Strengthening Foundations: Reflections on 2025 and What's Ahead for 2026

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    Blog Post
    ...We're expanding our collections strategically and forging new partnerships that will bring more tools to ...Frontiers in plant science, 16, 1517321. https://doi.org/10.3389/fpls.2025.1517321 Singh, O. N., Berry, ... SARS-CoV-2. iScience, 28(10), 113498. https://doi.org/10.1016/j.isci.2025.113498...
  29. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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    Blog Post
    ...and space. Zebrafish have become a popular model organism because their larval stage lends itself well to...toolbox and the versatility of the zebrafish model organism, the Isacoff lab was able to link these cells ... Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral service Find...
  30. Understanding GPCR Signaling Using TRUPATH

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    Blog Post
    ...transducerome. Nat Chem Biol 16:841–849 . https://doi.org/10.1038/s41589-020-0535-8 Takeda S, Kadowaki S,...genome sequence. FEBS Letters 520:97–101 . https://doi.org/10.1016/s0014-5793(02)02775-8 Additional resources...Find other fluorescent biosensors Resources on Addgene.org Browse the catalog of fluorescent protein plasmids...
  31. Build Your CRISPR Vocabulary

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    Blog Post
    ..., 641–656. https://doi.org/10.1038/s41579-022-00739-4 More resources on addgene.org  Addgene's CRISPR ...distinguishing the foreign sequences and keeping the array organized. Target strand: The strand of foreign DNA that...
  32. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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    Blog Post
    ...genome-wide, and has been widely applied in many model organisms and cell lines. Although ChIP-seq is a relatively...cells. However, when working on specific tissues or organs in whole animal models like Drosophila melanogaster...interactions with CAPTURE Additional Resources on Addgene.org Find simple lab protocols Browse CRISPR plasmids...
  33. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    ...done to and for us? BMC Biology, 15, 91. https://doi.org/10.1186/s12915-017-0433-z. PMID: 29052511. Louten...Replication. Essential Human Virology, 49. https://doi.org/10.1016/B978-0-12-800947-5.00004-1 Taylor, M. W...40). Springer International Publishing. https://doi.org/10.1007/978-3-319-07758-1_2 More resources on the...
  34. What the HEK?

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    Blog Post
    ... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral...and Troubleshooting Additional resources on Addgene.org AAV Production in HEK-293T Cells  ...
  35. Science Careers: Unruly Interests Feed Many Paths

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    Blog Post
    ...fascinating biology is found in weird and rare organisms so I found it hard to stick to vertebrates alone...afield at first and I let them pass by. But I didn’t forget: what would it be like? Then I spotted an opening...Subscribe to Career Blog Posts Resources at Addgene.org Find Job Openings at Addgene ...
  36. Identifying Sequence Elements with SnapGene's Feature Database

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    Blog Post
    ...feature database. Powering the plasmid maps on Addgene.org with SnapGene Server is useful because it helps...is to enhance Collections so that a group or organization can annotate their sequences from a shared database...sequencing at Addgene Additional Resources on Addgene.org Example plasmid analysis powered by SnapGene...
Showing: 401 - 440 of 965 results