...of CRISPR-Cas9 by CASANOVA Article contributed by Cary Valley  Listen to the anti-CRISPR podcast segment...

...incredibly dedicated team. Many thanks to Tyler Ford, Caroline LaManna, Joanne Kamens, Lenny Teytelman, April...

...necessary result of the #MeToo movement. We are carefully attentive to genuine inclusion, but not worried...

...different plasmid backbones that varied in size or carried different genes. In these cases, gene expression...

...mushy, gooey contents of said containers, think carefully about how you want your software to treat those...

...Sergi, Jacob J. Hughey, Bryce T. Bajar, Silvia Carrasco, and Markus W. Covert. "High-Sensitivity Measurements...

...Kovalski, J.R., Siprashvili, Z., Palmer, T.D., Carette, J.E., & Khavari, P.A. (2018). RNA–protein interaction...

...26615910. PubMed Central PMCID: PMC4674677. Ronda, Carlotta, et al. "Metagenomic engineering of the mammalian...

...distribute through our deposit process, and then we carefully track samples through our lab using barcoded tubes...

.... PubMed Central PMCID: PMC4139937. 3. Anders, Carolin, et al. "Structural basis of PAM-dependent target...

...like papers that can end with some sort of model (cartoons are appreciated) to visually sum up all of the...

...Addgene’s materials, and career advice. Playlists include: Protocols Careers How-to Blog Read about plasmids... in molecular biology, laboratory research, and career development. eBooks Download any of our eBooks ...Viral Vectors 101 Fluorescent Proteins 101 Science Career Guide Videos Watch our collection of video content...Proteins CRISPR Viral Vectors Plasmid Technology Career Readiness Molecular Biology Learn about plasmids...

...Harbor Laboratory Publications, New York. Helma J, Cardoso MC, Muyldermans S, Leonhardt H (2015) Nanobodies...

...cells. What’s more, G-baToN can be used to bring cargo, such as protein or DNA, to manipulate function ...

... active site in Casɸ can both process crRNA and carry out the crRNA-guided DNA cutting, unlike other well-characterized...

...significant contributions from Alyssa Cecchetelli, Cary Valley, and Holly McQueary. Resources Additional...

... developed a second set: The ABE8.x-d version carries a fusion of wild-type TadA and the evolved TadA...

...the virus so that it only enters and delivers it cargo to certain cell types.  Botond Roska’s lab at the...

...generally less common and will stand out; however, be careful not to hang your poster too low. If it is pinned...

... to make sure the deposit agreement/MTA is taken care of and, once we have all of your plasmid data, we...

...containing structural genes and a transfer plasmid carrying the transfer gene. By dividing the viral elements...

...lentiviral vector deposited by Dr. Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette...

...efficiently enter cells and deliver a variety of cargoes, genetically engineered neurotropic viruses emerged...

...Paquet, D., Teo, S. et al. Precise and efficient scarless genome editing in stem cells using CORRECT. Nat...

... - they can hold up to 8 kb, while AAV can only carry around 4.5 kb. Different AAV serotypes have preferences...

...for your genes of interest, your F1 mice will be carriers at those alleles. Some alleles, such as Cre, function...

...improved transcript life, and so should be handled with care (4). For this reason, viral vectors often employ...

... 4 °C prior to use. Thawed aliquots should be discarded after 1–2 months. 1 mg/mL polyethylenimine, linear...stored at 4 °C for up to 2 months. After 2 months, discard the tube and thaw a new working stock. Considerations...DNA tube. Incubate the mixture 15–20 min at RT. Carefully transfer the transfection mix to your HEK293T ...cells. Add the transfection mix dropwise, being careful not to dislodge the cells. Incubate the cells for...the following morning. The following morning, carefully aspirate the media. Replace the media with 15 ...

...move your microscope around the lab, be sure to carry it with two hands - one hand supporting the base...base and the other holding the arm - or use a cart. Revolve the nosepiece so that the lowest power objective...objective. Take your eyes away from the eyepiece and carefully revolve the nosepiece so that the next objective...will notice that the objectives get larger. Be careful to avoid hitting your sample with the objective...laboratory instrument, you should be sure to take care of your microscope. Bumps, scratches, and dust can...

...resolution of positive and negative populations, Careful panel design In both conventional and spectral ...

... plasmid collection Browse the RESOLUTE solute carriers collection Find other COVID-19 and Coronavirus...

...interest. They created a selection phage (SP) to carry catalytically dead SpCas9 (dCas9) fused to the bacterial...

...alter for a purpose and To direct the professional career of. Like everyone else I mostly learned through...

...now have another model of Cas9 – a flexible alpha-carbon backbone model made of nylon – that more accurately...

...the Biohub in San Francisco. Before starting his career in software, he completed a MSc in Psychology at...

...reviews journal. She joined Cell Press to pursue a career in science communication and publishing after completing...

...achieve greater efficacy, researchers will have to carry out repeated transfections with the ssDNA guide,...

...could be forming dimers in cells. Why should you care? Three simple ways a dimeric FP could ruin your ...

...your mutation. To maintain stable lines of flies carrying a specific mutation, Drosophila geneticists use...

...PMCID: PMC3074624. 5. Bindels, Daphne S., et al. "mScarlet: a bright monomeric red fluorescent protein for...

... the plasma membrane by fusing various vesicle cargoes to FP timer mk-GO (Tsuboi et al.) Monitor gene ...

...Pubmed. C. Soderblom, D.-H. Lee, A. Dawood, M. Carballosa, et al, (2015) eNeuro, March/April 2015, 2(2)...

...segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes...

...Golden Gate Modular Cloning Toolbox for Plants. Carola Engler, Mark Youles, Ramona Gruetzner, Tim-Martin...

...many genes simultaneously. These parts have been carefully engineered to support reliable functional composition...

...the second stop and carefully raise the pipette straight out of the buffer. Carefully load your samples ...occasionally as the solution heats up.). Caution HOT! Be careful stirring, eruptive boiling can occur. Pro-Tip It...not added EtBr to the gel in the first place. Carefully load a molecular weight ladder into the first ...the electrodes from the power source, and then carefully remove the gel from the gel box. Optional: If ...

...2007-02-062117. PMID: 17510320. Tollefson, A. E., Scaria, A., Hermiston, T. W., Ryerse, J. S., Wold, L. ...

...distortions to a minimum with protocol optimization and careful execution, but they cannot be entirely eliminated...

...hours, so read up on the timing of this approach carefully (Lin et al., eLife)! Use a Cas that facilitates...

...science and society." Science 376, (2022): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter...