We narrowed to 1,081 results for: ANT

...Tagging of Endogenous Genes with SapTrap Even more elegant: Single injection CRISPR/Cas9 in C. elegans External...

...PCNA-mEGFP AICSDP-116 mEGFP Proliferating cell nuclear antigen (PCNA) DNA replication foci IPSC cell lines can...

... Lambright, D. G., Lee, F. S., Randazzo, P. A., Santy, L. C., Schürmann, A., Wilhelmi, I., Yohe, M. E....

...enzymes for cutting protein sequences to remove unwanted parts, and DNA-binding proteins that can be fused...

...working with INTRSECT recombinases, viruses, and important controls to consider as part of experimental design...

...B. K., Wong, S. H., Wong, T. Y., Yang, G. S., Ypsilanti, A. R., Jones, S. J., Holt, R. A., Goldowitz, ...

... viral prep format. Please note this does not guarantee viral vector packaging service, but lets us know...

...dynamics of KRAS , p53 and LKB1 , the top three significantly mutated genes in lung adenocarcinoma. Delivery...

...AiE0410m Cre Oligodendrocytes Whole Brain * = Substantial non-specific labeling observed Publications Ben-Simon...

...available in viral format. Please note this does not guarantee viral service, but lets us know what viruses would...

... are called recombinant antibodies. Recombinant antibodies are created by cloning antibody genes from ...Science Guides Antibody Guide Antibody Guide View Addgene's Antibody Collection Jump To: Antibody Guide Production... produce antibodies, with the highest variability in antibodies produced. Cells - Antibodies can also ...amounts of a single antibody with little variation. Recombinant plasmids - Antibodies can be produced from...variable region and constant regions of the antibody to be independently manipulated. Antibodies produced this... in a buffer. Antibody Structure Figure 1: Structure of an antibody A standard antibody is made up of ...producing antibodies, can change the constant region, and therefore change the isotype of antibodies they ...

...Recommended controls for antibody-based experiments Primary antibody specificity Antibody-based assays have ...Microscopy-based antibody experiments should include a primary antibody specificity control, secondary antibody specificity...species as your primary antibody, like if you are using an anti-mouse IgG antibody in mouse tissue. Such... signal from your anti-Mouse IgG2a secondary antibody, then that secondary antibody is reacting non-specifically...Addgene blog Antibodies 101: Validation Antibodies 101: Choosing the Right Isotype Antibodies 101: Selecting...confirm primary antibody specificity. The gold standard in demonstrating primary antibody specificity is...approaches discussed in the Antibodies 101: Validation post. The primary antibody control needs only to be...

...: Use healthy plants: Your plants should show no signs of stress, such as anthocyanin production, or ... more chances to recover a transformant. Use lots of plants: Lots of plants produce lots of seeds. I typically...select for transformants. It’s very important to select multiple T1 plants from each transformation. I like...process itself can affect the growth of resistant plants so you’ll want to know the effect of your insertion...of non-transformants and make finding true transformants more difficult. Don’t leave your plants on selective...dynamics of genes and proteins in living plants, to complementing mutant phenotypes. Arabidopsis is amenable...genomes of the plants it attacks Growing the transformed agrobacterium culture Dipping your plant’s flowers ...

...smaller antibodies.They are derived from an unusual type of IgG antibody called a heavy chain antibody (HCab...traditional IgG antibody structures. Functional comparison Strong but Large: Advantages of Antibodies One of ...different epitopes of a primary antibody. B) Each arm of the antibody has an antigen binding site for a total... (DSHB). 1 ml of antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially...require secondary antibodies, frequently of the mouse or rabbit variety. While antibodies certainly aren’...their anti-mouse and -rabbit IgG secondary nanobodies toolbox. Nanobodies are like tiny antibodies which...be attached to each antibody. But being big is a double-edged sword. An antibody’s large size creates ...

...10,000 for primary antibodies). You’ll want to run a protein gradient to test the antibody at the recommended...polyclonal or recombinant multiclonal antibodies to increase overall signal from the primary antibody.  Pro tip... Not enough antibody binding sites Polyclonal or recombinant multiclonal antibodies Protein not... your blocking, first check your antibody spec sheet. Some antibodies work better with specific blockers...issues.   If your antibody information doesn’t contain blocker recommendations, you may want to try different...make up a fresh batch.  Antibodies We always recommend either using an antibody validated for your protein...interest or validating an antibody yourself. But even with a validated antibody, it can still take time ...

...protocol video!  Antibodies The most important part of your western is your antibodies, particularly your...linearity within detection limits (important if you want to quantify your blots). Fluorescent proteins ...inherently “fuzzier” than gels. Quantification If you want to quantify the amounts of protein in your ...specificity Monoclonal antibodies Increased cost Recombinant antibodies Not always available;...your primary antibodies. We always recommend using an antibody validated for your target in your specific...polyclonal and monoclonal antibodies can work well for a western blot. Polyclonal antibodies, which bind to multiple... The highest reproducibility comes from recombinant antibodies (rAbs), which are produced from plasmids...

...labeled anti- HA or -MYC antibody or with an anti-HA or -MYC primary and a labeled secondary antibody. While...scale production of antibodies. Problems with antibodies Conventional antibodies are a non-renewable...with a labeled anti-HA or -MYC antibody, approximately as sensitive as detecting antigen with a standard... cross-react with anti-rodent or -rabbit Detection with an anti-Chicken IgY antibody is approximately ... (DSHB). 1 ml of antibody-containing hybridoma supernatant is only $40, significantly cheaper than commercially...Genetic Tagging - Since antibodies are produced in animals instead of via recombinant DNA, they can’t be genetically...below. The HA tag can be stained for with an anti-HA antibody and serves as a backup means of detection ...

...providers have moved to producing anti-Myc [9E10] as a recombinant antibody. Researchers first sequenced the...blog Antibodies 101: Monoclonal Antibodies Plasmids 101: Peptide Tagging Plasmid-based Recombinant Antibody...? Why Myc? When did the commonly used anti-c-Myc [9E10] antibody come into play? We’ll dive into those...monoclonal antibodies. However, it was (and still is) infeasible to generate highly specific antibodies for ... peptide. One of these C-terminal antibodies was Myc1-9E10 (aka anti-c-Myc [9E10]). They noticed that ...found that the N-terminal half of the antigen differs significantly between human and the other species,...generated their Myc antibodies. At first, anti-c-Myc [9E10] was just one of several new tools for unraveling...

...purple plant pigments found in all other plants, anthocyanins. Figure 1: An adult plant expressing...resistance to an antibiotic. Successful editing of transformants then typically occurs in T0 plants (from callus...an Arabidopsis mutant that can’t set seeds. I study the development of the mutant plants, but the seeds...unfamiliar with these mutant plants. Using RUBY to simplify the identification of these mutants will speed up ...expressing RUBY plant is a vibrant red/magenta while (bottom panel, left) a lower expressing RUBY plant is a pale...transgene markers for plant transformation fall into two main categories: antibiotic/herbicide resistance...off-target gene editing. Antibiotic selection would only then kill the desired plants! PCR or fluorescent ...

...Endothelial-specific AAV delivery Recombinant anti-FLAG tag antibodies Peptide-assisted genome editing...-023-38582-7. PMID: 37291094.   Recombinant anti-FLAG tag antibodies By Ashley Waldron Of all the epitope... a variety of applications. Find anti-DYKDDDDK recombinant antibodies here!... generated a series of StayGold mutants and identified a triple mutant, Y187F, R144I and T155Q, which ...library of AAV9 variants (Chuapoco et al., 2023). They found the best-performing variant, CAP-Mac, was ...compared to AA9-X1.1. Importantly, AAV1-X1 is not neutralized by pre-existing antibodies against AAV9-based...you know that we distribute ready-to-use recombinant antibodies as well? Our collection includes a mouse...