Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 499 results for: Ank;

Showing: 201 - 220 of 499 results

  1. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type
    Blog Post
    ...resistance (Figure 2). This MazF-ZeoR cassette is flanked by two direct repeats which can be used for marker...through a second recombination event between the two flanking repeats. In cells where recombination has not ...

  3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target, but many...018-1591-1 Cox DBT, Gootenberg JS, Abudayyeh OO, Franklin B, Kellner MJ, Joung J, Zhang F (2017) RNA editing...

  4. Bioinformatics at Addgene

    Type
    Blog Post
    ...large formats, such as 96-well microplates, and thanks to our dedicated product and development teams,... shared resource of pipelines for genomic data. Thanks to the flexibility inherent in Nextflow’s design...

  5. The Challenges of Cell Culture

    Type
    Blog Post
    ...or virology without acquiring materials from this bank. I am often asked, “What is the difference between...their identity. For example, perhaps a working cell bank has been made by a research institution (an excellent...

  6. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...transfer plasmid encodes your insert of interest, flanked by long terminal repeat (LTR) sequences, which ...Mansour, M. R., Schwarzwaelder, K., Bartholomae, C., Hubank, M., Kempski, H., Brugman, M. H., Pike-Overzet,...

  7. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... there is at least ~200 base pairs of sequence flanking the editing site on either side of the amplicon...screening by PCR may be the way to go. Design a PCR to flank your desired edit site and run the PCR product on...

  8. March for Science

    Type
    Blog Post
    ...staff. Many thanks to our guest blogger, Stephanie Hays. Stephanie would like to additionaly thank Isaac Plant...

  9. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...the addition of gene-specific 1 kb homology arms flanking a FLOXed neoR gene. pAAV-SEPT-Acceptor, the preferred... to deposit any new AAV tools you create. Many thanks to our guest blogger, Todd Waldman!  Todd Waldman...

  10. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...Also shown are the “B” restriction sites, which flank the targeted region. Upon PCR amplification, the...minor modifications to a plasmid template. Many thanks to our Guest Blogger Kristian Laursen! Kristian...

  11. Evolution of Lab Techniques

    Type
    Blog Post
    ...Technique" in the comments section below! Many thanks to our guest blogger Krissy Lyon! Krissy Lyon is...32. PubMed PMID: 6176866. 3. Hoebeeck, Jasmien, Frank Speleman, and Jo Vandesompele. "Real-time quantitative...

  12. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    ...Duchenne muscular dystrophy. gRNAs can be designed to flank a single or multiple exons that, when deleted through...low to no off-target activity at the ten highest-ranking predicted off-target sites. Each paper characterizes...

  13. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...: Key elements (promoter, tag, polyA site) are flanked by unique restriction sites so you can swap sequences...endonuclease-reverse transcriptase uses homology arms flanking an RNA payload to insert the payload sequence ...

  14. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    ...Hippocampus labeled with anti-Kv2.1 (magenta), anti-AnkyrinG (green), and Hoechst nuclear stain (cyan). Panels...Immunofluorescence Staining. In W. H. Yong (Ed.), Biobanking (Vol. 1897, pp. 299–311). Springer New York. ...

  15. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...cost-effective and efficient manner than ever before, thanks to the development of site-specific nucleases (...the pitfalls common to other approaches. Many thanks to our Guest Bloggers Amar Singh and Mathew Goodwin...

  16. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    ...calculated p values from smallest to largest and ranking them (1 being the smallest value). It calculates...significance by finding the largest p value where its rank divided by the total number of comparisons performed...

  18. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...usually the products of teams: Watson, Crick, & Franklin, Meselson-Stahl, Hershey-Chase...Who was I to ...Discovery is exactly that: a myth. The original Dr. Frankenstein from Mary Shelley’s novel worked alone, but ...

  19. Finding Your Science Policy Path

    Type
    Blog Post
    ...networking---and they’re more accessible than ever thanks to the virtual world that the pandemic has foisted.... Image used with permission from SCI. Many thanks to our guest blogger Steph Guerra, a AAAS Science...
Showing: 201 - 220 of 499 results