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  1. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...contaminants with lower densities, including empty capsids. The 60% step serves as a cushion for genome-containing... number of contaminants in each fraction. * AAV capsid proteins VP1, VP2, VP3; M protein marker. Image...

  2. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...you are using a polymerase with proofreading capabilities. Note: See manufacturer’s instructions for specific... of the reaction mix. Its solvent and buffer capabilities provide a suitable environment for the DNA amplification...

  3. Western Blot

    Type
    Protocol
    ...Microcentrifuge tubes BCA Assay, Thermo Fisher 23227 β-mercaptoethanol 4X protein loading buffer Precast SDS-PAGE ...sample for loading as follows: Add 10% v/v β-mercaptoethanol to the 4X protein loading buffer. Dilute 4X...

  6. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    ...CRISPR, Addgene gave to me: Selective Endogenous eNcapsidation for cellular Delivery (SEND). On the fourth ...

  7. Addgene's 20th Anniversary Party

    Type
    Blog Post
    ...photos and slideshows shared some of the moments we captured over the years, including an album of Addgenie...

  10. Summer Fun at Addgene!

    Type
    Blog Post
    ...haven’t seen our webinar “Navigating an Expanding Landscape for Engineered Systemic AAVs” with the director...
Showing: 201 - 220 of 401 results