...plasmid aka the RepCap plasmid encodes two genes: the replication (rep) gene and the capsid (cap) gene. In ...into a capsid other than AAV2. This production strategy is called cross-packaging.  Capsid (cap) gene ...take a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products. Created...gene The cap gene encodes three AAV viral capsid proteins VP1, VP2, and VP3, which together form the outer...outer capsid shell that protects the viral genome. The cap gene is often driven by the AAV p40 promoter....variable loops is one method for changing a capsid’s tropism.  cap also encodes the Assembly-Activating Protein...recombinant AAV (rAAV) is made in the lab, rep and cap are expressed from a separate plasmid in trans, freeing...

...systemic capsids. After some consideration, we offer this definition: A systemic capsid is a capsid that ... Image and caption adapted from Campos et al., 2023. AAV.CAP-Mac is a systemic capsid, developed ...may want to consider a systemic capsid for your AAV.   Systemic capsids While there is quite a bit of information...Image and caption courtesy of Challis et al., 2022. Where do they come from? Systemic capsids are typically...new systemic capsids for both rodents and non-human primates (NHPs). These engineered capsids have increased... the systemic capsid in question (or both). Therefore, a high-efficiency systemic capsid has better efficacy...precursor. Considering systemic capsids for your research? While systemic capsids mostly function like your ...

...earned a blue flame for its adenine base editing capabilities. Epigenetics: CRISPR has made it possible to...resources. Not on social media? No problem - see our recap below! Day 1: gRNA design tools: How to Design your...

... Figure 2: Comparing CAPTURE and CAPTURE 2.0.     Original CAPTURE CAPTURE 2.0 Biotinylation ...between original CAPTURE and CAPTURE 2.0 methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased.... Find plasmids for CAPTURE 2.0! When compared to the original CAPTURE, CAPTURE 2.0 had a ~14-fold increase...Recently the Xu lab developed CAPTURE 2.0, an updated version of CAPTURE that detects chromatin interactions...Regulatory Elements: The original CAPTURE method The original CAPTURE method requires the creation of a.... From here, the CAPTURE method is similar to other 3C methods: crosslinking captures chromatin interactions...the use of CAPTURE with primary cells or rare cell populations. Key components of the CAPTURE 2.0 system...

...impact on this challenge either for magnetic capture or for capture using microfluidic devices. Addgene: What...S” in Streptococcus and the “py” in pyogenes), capable of locking two peptide tags together. SpyLigase...antibodies against common tumor markers to subsequently capture cancerous cells expressing low levels of tumor ...binding stability really mattered for efficient capture. We wanted to be able to build a highly multivalent...for keeping going while we worked out the cell capture system.) Cancer and other applications Addgene:...technology mean in the context of cancer? Howarth: The capture of circulating tumor cells (CTCs) is going to have...treatment of cancer. It is clear that one needs to capture a diverse range of CTCs to cope with the variation...

...injecting CAP-B10 or CAP-B22 in marmosets, from an individual to another individual perspective? A: CAP-B10 ...CAP-B10 and CAP-B22 were tested individually in 4 marmosets each. Brain potency for each individual is reported...would you recommend using the PHP.S capsid over the PHP.eB (the capsid we have been using)?Would you recommend... insert with the CMV promoter using the AAV-BR1 capsid? A: It is our understanding that AAV-BR1 has the...recommend any liver specific capsid, particularly one that can target the epithelial cells in the blood-brain... AAV9 or AAV8. Very recently an engineered AAV5 capsid was reported for specific and potent liver transduction...recommend you keep an eye out for emerging new capsids in this area.   Q8: On dense labeling in the brain...

...mutant AAV capsid (cap) expressing plasmids is generated. Diversity is introduced into the cap plasmid library...combinations into a region of the cap gene. In this special CREATE cap plasmid, loxP sites flank the downstream...especially for the study of pain. While many new capsids (i.e. the part of the virus that determines tropism...CREATE method to engineer two new IV injectable AAV capsids, AAV.PHP.eB and AAV.PHP.S, that target the CNS ...  Read on to learn more about these two new AAV capsids and how they can be useful tools for studying the...pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell type of interest...rounds of infection and screening by PCR enrich for capsid variants with increased ability to infect the cell...

... follows: Cap Subject Industry H1B Academic H1B National Interest Waiver EB-1A EB-1B Cap subject industry...These numerically limited visas are referred to as “cap” subject H-1Bs. The H-1B allows U.S. employers to...application on their behalf.  If an individual obtains a cap subject H-1B (last year less than 30% of applicants...Four types of employers are not subject to the H-1B cap: institutions of higher education nonprofit entities...employers. H-1B petitioners are exempt from the cap if the applicant works at institutions of higher ...boutique consulting, investment banking and venture capital areas.  We place individuals into roles in areas...

...integration at the AAVS1 locus in humans. Cap is a structural capsid protein that helps with virion assembly...The viral packaging proteins for AAV are rep and cap. Rep is required for viral replication and also aids...from late endosomes when AAV is trafficked. Rep and cap mediate AAV pseudotyping which allows for altered...Gag proteins: they contain the matrix, capsid, and nucleocapsid viral components needed for viral packaging...other viruses discussed. LXs/Iva2 are primarily capsid assembly proteins required for packaging of the...

...and help keep things more organized. Cap tubes with a micronic cap sealer. This is a simple table top device... device that has a roller to go over caps and push them into micronic tubes instead of pushing them in...

...10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with ..., 2023). They found the best-performing variant, CAP-Mac, was effective at breaching the blood-brain barrier... macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters... for primary cells Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains... an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression...engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in ... primate (NHP) brains.  Systemic gene delivery capsids previously identified in rodents have not been ...

...only 1.5 ml of each are used). This solution is capped, swirled and tested for the correct temperature...and not spread out onto the plate. We test the (capped) 1:1 agarose/DMEM solution against a part of the...

...Translation in eukaryotes usually begins at the 5’ cap so that only a single translation event occurs for...spark translation initiation independent of the 5’ cap.  IRES elements are very useful and commonly found... viral transfer vectors with limited packaging capacity. Additionally, it may not be feasible to express...

..._fang) That’s right, researchers of the world. Cap your Eppendorf tubes, hang up your pipettes, and ...

...last base pair with an end-capping loop (Nishimasu et al., 2014). This cap is where the DNA:RNA heteroduplex...lobe has been hidden for visibility. A RuvC loop caps the heteroduplex and marks the transition back to... cuts.   Pro tip! X-ray crystallography cannot capture flexible or disordered regions, so crystal structures...

...addressing the new funding structures and indirect cost caps more efficiently. Offering free educational resources...

...Actin, cyan), with formin (649-mDia1, magenta) and capping protein (549-CP, yellow) at the tip. 649-mDia1 ...actin barbed end assembly by twinfilin, formin and capping protein. Nature Communications, 14(1), Article ... workflows you already use. Or you can really capitalize on the dye’s performance and use super-resolution...

...arabinose, cAMP complexed with cAMP activator protein (CAP) can also stimulate AraC binding to I1 and I2 sites...

...conjunction with the viral rep (replication) and cap (capsid) proteins, which bind to the ITRs and initiate... varying capacities. AAV vectors containing the AAV2 ITRs can be cross-packaged with capsids from multiple...., 2019), and different combinations of ITR and capsid serotypes vary in their gene transfer efficiency... 101: Pseudotyping Viral Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide...2023). AAV- based vector improvements unrelated to capsid protein modification. Front Med, 10, 1106085. https...