...Pipette 0.5 mL of Tween-20 to into 999.5 mL 1X PBS Cap the bottle and invert several times to mix. Carefully...) as follows: Add 250 mg of BSA to 25 mL of PBS. Cap the tube and invert several times to mix. Using a...PBS) as follows: Dilute 50 mg BSA into 50 mL PBS . Cap the tube and invert several times to mix. Dilute ...starting with three concentrations between 1–10 µg/mL. Cap and invert the tubes several times to mix. Using ... concentrations in the antibody dilution buffer. Cap and invert the tube several times to mix. Using a...mL Peroxide Solution from the TMB substrate kit. Cap the tube and invert several times to mix. Using a...

...Transfer 6 mL of BCD TFX into each of two 50 mL tubes. Cap the tubes and incubate for 1 h in the 37 °C bead ...antibody plasmid DNA to one tube of 6 mL BCD TFX. Cap the tube and vortex for 5 s to mix. Add 450 µL of.../mL stock solution of PEI-MAX, 450 µg = 450 µL). Cap the tube and vortex for 5 s to mix. Pro-Tip The optimal...1:6. Add the diluted PEI-MAX to the diluted DNA. Cap the tube and vortex with three 1 s pulses. Incubate... 12 mL of PEI-MAX/DNA mix to the flask dropwise. Cap the flasks and swirl 5–10 times to mix. Return the...

...tubes. Cap the Gravitrap columns at the bottom. Add 5 mL of Pierce IgG Elution Buffer to the capped Gravitrap...containing 50 µL 1 M Trizma hydrochloride pH 9.0. Cap the tubes and vortex for 5 s to mix. Determine the...Spin Desalting Column’s bottom closure and loosen cap. Place column in a 50 mL conical collection tube....columns have a finite binding capacity. If your sample exceeds the capacity, divide the sample among multiple...1 part tissue culture supernatant and mix well. Uncap the Protein G Gravitrap or rProtein A Gravitrap ...columns. For information about the total binding capacity for your columns, refer to the manufacturer’s ...Gravitrap column and incubate for 10 min at RT . Uncap the column and collect 500 µL fractions into the...

...(top) and with growth (bottom) Loosely close the cap on the bottle (do NOT close all the way or the bottle...cover the culture with sterile aluminum foil or a cap that is not air tight. Incubate bacterial culture...specific plasmid. However, a liquid culture is capable of supporting a higher density of bacteria and ...

... drops along the side of the tube. Cap gently - do not push the cap in all the way, just ensure the samples...PCR tubes. Pipette back and forth 5 times. Lightly cap the PCR tubes. Generate the Droplets Bring the PCR...Without disturbing the liquid in the tube, gently uncap the PCR tubes. Add 5 µL of dilutions 6–8 to the ...Without disturbing the liquid in the tube, gently uncap the tubes. Add 5 µL of the previously aliquoted ...

...such that the media goes toward the cap. If the media touches the cap, replace it with a new one before ...optimized. Plasmids for transfection: pHelper; pRC (Rep-Cap), plasmid expressing your gene of interest Triton...bp) DNA Concentration (μg/μL) Volume of DNA (μL) RepCap 7,265 1.00 727.6 pHelper 11,854 1.00 1,185 Transfer..., for each plasmid we need: Sample Calculations RepCap: 0.08 μg/bp × 7,265 bp = 582.1 μg Volume Needed...

...colloid. Cover the opening of the bottle with its cap or aluminum foil (but do not make an air-tight seal...distrubtion of agar over the bottom of the plate. Cap each plate after pouring and stack as you pour. If...that way for ~10 min. This will allow any steam to escape from the autoclave and will cool your gel-mix slightly...

...Procedure The balance in the photo above has a capacity of 200 g, as indicated by the number above the...is best. Check that the balance has the correct capacity for the material that you are weighing by looking... you’re weighing out an amount larger than the capacity of your balance or weighing boat, you can weigh...

...Addgene's video content, including how-to screen captures, lab procedure protocols, and career advice videos...standard laboratory procedures. How-To Videos Screencapture walk-throughs to help guide you through Addgene's...

...digital cameras attached to them that allow you to capture images directly to a computer, but many microscopes... your drawing skills. Regardless of your image capture method, be sure to record the magnification used...

...contaminants with lower densities, including empty capsids. The 60% step serves as a cushion for genome-containing... number of contaminants in each fraction. * AAV capsid proteins VP1, VP2, VP3; M protein marker. Image...

...you are using a polymerase with proofreading capabilities. Note: See manufacturer’s instructions for specific... of the reaction mix. Its solvent and buffer capabilities provide a suitable environment for the DNA amplification...

...Microcentrifuge tubes BCA Assay, Thermo Fisher 23227 β-mercaptoethanol 4X protein loading buffer Precast SDS-PAGE ...sample for loading as follows: Add 10% v/v β-mercaptoethanol to the 4X protein loading buffer. Dilute 4X...

... of a regular nucleotide for broader pairing capabilities. Taking into consideration the information above...

...bath, and be sure to know what the maximum fill capacity is. Look for a “fill” line inside the water bath...

... of and gently in contact with the container (capillary action between the container and the liquid will...

...vol) N-laurosylsarcosine (Sarkosyl) 0.1 M 2-mercaptoethanol TRIzol® or similar product such as TRI Reagent...

...overloaded. If you find that your pellet outstrips the capacity listed in the Maxiprep protocol, scale the reagent...

... total, and the stuffer insert is shown in all capital letters. I.2. Recipes Luria Broth Agar (LB agar...