...PHP.V1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175004 pUCmini-iCAP-AAV.CAP-B10 ... B10 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 175005 pUCmini-iCAP-AAV.CAP-B22 ...and PHP.eB Cap controlled by tTA-TRE amplification system Viviana Gradinaru 103006 pUCmini-iCAP-PHP.S PHP.S...and PHP.S Cap controlled by tTA-TRE amplification system Viviana Gradinaru 127847 pUCmini-iCAP-PHP.V1 PHP.V1... and B22 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185136 pUCmini-iCAP-AAV.MaCPNS1... MaCPNS1 Cap controlled by tTA-TRE amplification system Viviana Gradinaru 185137 pUCmini-iCAP-AAV.MaCPNS2...plasmid (non-standard), expressing Rep2 and MaCPNS2 Cap controlled by tTA-TRE amplification system Viviana...

....V1 MaCPNS1 MaCPNS2 CAP-B10 CAP-B22 AAV9-X1.1 As part of our Viral Vector Packaging Service , Addgene ...Pubmed 35643078 (Link opens in a new window) CAP-B10, CAP-B22: Goertsen, Flytzanis, et al., 2022 Pubmed...Control Boyden CAP-B10 These viral vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B10 plasmid...Control Boyden CAP-B22 These viral vector preparations were produced with the pUCmini-iCAP-AAV.CAP-B22 plasmid...plasmid (Addgene #175004) . Browse Available CAP-B10 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP...plasmid (Addgene #175005) . Browse Available CAP-B22 AAV ID Name Promoter Description Category PI 37825 pAAV-CAG-GFP...AAV Caltech Systemic Capsids Viral Vector Packaging Service: Caltech Systemic Capsids PHP.eB PHP.S PHP.V1...

... and human Jonathan Ting Viviana Gradinaru 37825-CAP-B10 pAAV-CAG-GFP AAV expression of GFP from the CAG...CAG promoter Edward Boyden Viviana Gradinaru 37825-CAP-B22 pAAV-CAG-GFP AAV expression of GFP from the CAG...CAG promoter Edward Boyden Viviana Gradinaru 37825-CAP-MaCPNS1 pAAV-CAG-GFP AAV expression of GFP from the...CAG promoter Edward Boyden Viviana Gradinaru 37825-CAP-MaCPNS2 pAAV-CAG-GFP AAV expression of GFP from the... Preps ID Viral Prep Description PI (Cargo) PI (Capsid) 214869-PHPeB AiP15140: pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3...

...AAV9 AAVrh10 Retrograde AAV PHP.eB PHP.S PHP.V1 CAP-B10 CAP-B22 MaCPNS1 MaCPNS2 AAV9-X1.1 Promoter CAG CaMKIIa... GFP Constitutive 1, 2, 5, 8, 9, rg*, PHPeB, CAP-B10, CAP-B22, MaCPNS1, MaCPNS2, AAV9-X1.1 Boyden 44332...

...neurons Caltech Systemic Capsids PHP.eB, PHP.S, PHP.V1, MaCPNS1, MaCPNS2, CAP-B10, CAP-B22 University of Florida...

...plasmid aka the RepCap plasmid encodes two genes: the replication (rep) gene and the capsid (cap) gene. In ...into a capsid other than AAV2. This production strategy is called cross-packaging.  Capsid (cap) gene ...take a closer look at the rep and cap genes. Figure 1. The RepCap plasmid and its products. Created...gene The cap gene encodes three AAV viral capsid proteins VP1, VP2, and VP3, which together form the outer...outer capsid shell that protects the viral genome. The cap gene is often driven by the AAV p40 promoter....variable loops is one method for changing a capsid’s tropism.  cap also encodes the Assembly-Activating Protein...recombinant AAV (rAAV) is made in the lab, rep and cap are expressed from a separate plasmid in trans, freeing...

...systemic capsids. After some consideration, we offer this definition: A systemic capsid is a capsid that ... Image and caption adapted from Campos et al., 2023. AAV.CAP-Mac is a systemic capsid, developed ...may want to consider a systemic capsid for your AAV.   Systemic capsids While there is quite a bit of information...Image and caption courtesy of Challis et al., 2022. Where do they come from? Systemic capsids are typically...new systemic capsids for both rodents and non-human primates (NHPs). These engineered capsids have increased... the systemic capsid in question (or both). Therefore, a high-efficiency systemic capsid has better efficacy...precursor. Considering systemic capsids for your research? While systemic capsids mostly function like your ...

...earned a blue flame for its adenine base editing capabilities. Epigenetics: CRISPR has made it possible to...resources. Not on social media? No problem - see our recap below! Day 1: gRNA design tools: How to Design your...

... Figure 2: Comparing CAPTURE and CAPTURE 2.0.     Original CAPTURE CAPTURE 2.0 Biotinylation ...between original CAPTURE and CAPTURE 2.0 methods.   Applications of CAPTURE 2.0 CAPTURE 2.0’s increased.... Find plasmids for CAPTURE 2.0! When compared to the original CAPTURE, CAPTURE 2.0 had a ~14-fold increase...Recently the Xu lab developed CAPTURE 2.0, an updated version of CAPTURE that detects chromatin interactions...Regulatory Elements: The original CAPTURE method The original CAPTURE method requires the creation of a.... From here, the CAPTURE method is similar to other 3C methods: crosslinking captures chromatin interactions...the use of CAPTURE with primary cells or rare cell populations. Key components of the CAPTURE 2.0 system...

...impact on this challenge either for magnetic capture or for capture using microfluidic devices. Addgene: What...S” in Streptococcus and the “py” in pyogenes), capable of locking two peptide tags together. SpyLigase...antibodies against common tumor markers to subsequently capture cancerous cells expressing low levels of tumor ...binding stability really mattered for efficient capture. We wanted to be able to build a highly multivalent...for keeping going while we worked out the cell capture system.) Cancer and other applications Addgene:...technology mean in the context of cancer? Howarth: The capture of circulating tumor cells (CTCs) is going to have...treatment of cancer. It is clear that one needs to capture a diverse range of CTCs to cope with the variation...

...injecting CAP-B10 or CAP-B22 in marmosets, from an individual to another individual perspective? A: CAP-B10 ...CAP-B10 and CAP-B22 were tested individually in 4 marmosets each. Brain potency for each individual is reported...would you recommend using the PHP.S capsid over the PHP.eB (the capsid we have been using)?Would you recommend... insert with the CMV promoter using the AAV-BR1 capsid? A: It is our understanding that AAV-BR1 has the...recommend any liver specific capsid, particularly one that can target the epithelial cells in the blood-brain... AAV9 or AAV8. Very recently an engineered AAV5 capsid was reported for specific and potent liver transduction...recommend you keep an eye out for emerging new capsids in this area.   Q8: On dense labeling in the brain...

...mutant AAV capsid (cap) expressing plasmids is generated. Diversity is introduced into the cap plasmid library...combinations into a region of the cap gene. In this special CREATE cap plasmid, loxP sites flank the downstream...especially for the study of pain. While many new capsids (i.e. the part of the virus that determines tropism...CREATE method to engineer two new IV injectable AAV capsids, AAV.PHP.eB and AAV.PHP.S, that target the CNS ...  Read on to learn more about these two new AAV capsids and how they can be useful tools for studying the...pre-designed primers to determine which version(s) of the capsid gene successfully infected the cell type of interest...rounds of infection and screening by PCR enrich for capsid variants with increased ability to infect the cell...

... follows: Cap Subject Industry H1B Academic H1B National Interest Waiver EB-1A EB-1B Cap subject industry...These numerically limited visas are referred to as “cap” subject H-1Bs. The H-1B allows U.S. employers to...application on their behalf.  If an individual obtains a cap subject H-1B (last year less than 30% of applicants...Four types of employers are not subject to the H-1B cap: institutions of higher education nonprofit entities...employers. H-1B petitioners are exempt from the cap if the applicant works at institutions of higher ...boutique consulting, investment banking and venture capital areas.  We place individuals into roles in areas...

...integration at the AAVS1 locus in humans. Cap is a structural capsid protein that helps with virion assembly...The viral packaging proteins for AAV are rep and cap. Rep is required for viral replication and also aids...from late endosomes when AAV is trafficked. Rep and cap mediate AAV pseudotyping which allows for altered...Gag proteins: they contain the matrix, capsid, and nucleocapsid viral components needed for viral packaging...other viruses discussed. LXs/Iva2 are primarily capsid assembly proteins required for packaging of the...

...and help keep things more organized. Cap tubes with a micronic cap sealer. This is a simple table top device... device that has a roller to go over caps and push them into micronic tubes instead of pushing them in...

...10.21203/rs.3.rs-3188559/v1.  Neurogenetic alchemy: CAP-Mac unleashes the secrets of primate brains with ..., 2023). They found the best-performing variant, CAP-Mac, was effective at breaching the blood-brain barrier... macaques and vascular bias in mature marmosets. CAP-Mac also robustly expressed fluorescent reporters... for primary cells Monomeric StayGold variants AAV.CAP-Mac for systemic gene transfer in primate brains... an “assist peptide” that facilitates endosomal escape. Combining Cas9-PAGE with lentiviral sgRNA expression...engineered adeno-associated virus (AAV) vector named AAV.CAP-Mac for non-invasive systemic gene delivery in ... primate (NHP) brains.  Systemic gene delivery capsids previously identified in rodents have not been ...

...Pipette 0.5 mL of Tween-20 to into 999.5 mL 1X PBS Cap the bottle and invert several times to mix. Carefully...) as follows: Add 250 mg of BSA to 25 mL of PBS. Cap the tube and invert several times to mix. Using a...PBS) as follows: Dilute 50 mg BSA into 50 mL PBS . Cap the tube and invert several times to mix. Dilute ...starting with three concentrations between 1–10 µg/mL. Cap and invert the tubes several times to mix. Using ... concentrations in the antibody dilution buffer. Cap and invert the tube several times to mix. Using a...mL Peroxide Solution from the TMB substrate kit. Cap the tube and invert several times to mix. Using a...

...Transfer 6 mL of BCD TFX into each of two 50 mL tubes. Cap the tubes and incubate for 1 h in the 37 °C bead ...antibody plasmid DNA to one tube of 6 mL BCD TFX. Cap the tube and vortex for 5 s to mix. Add 450 µL of.../mL stock solution of PEI-MAX, 450 µg = 450 µL). Cap the tube and vortex for 5 s to mix. Pro-Tip The optimal...1:6. Add the diluted PEI-MAX to the diluted DNA. Cap the tube and vortex with three 1 s pulses. Incubate... 12 mL of PEI-MAX/DNA mix to the flask dropwise. Cap the flasks and swirl 5–10 times to mix. Return the...

...plasmid, a plasmid encoding Rep and serotype-specific Cap genes, and a plasmid encoding adenoviral helper sequences...intensity of the viral capsid proteins are analyzed. The abundance of viral capsid proteins as a fraction...the relative stoichiometric ratios of the viral capsid proteins VP1, VP2 and VP3. Samples of viral preparations...non-purified AAV subjected to gel electrophoresis. Viral capsid proteins VP1, VP2, and VP3 are shown relative to...