...the two ITRs, and Rep and Cap are supplied in trans . In addition to Rep and Cap, AAV requires a helper ...genome consists of two open reading frames, Rep and Cap, flanked by two 145 base inverted terminal repeats...synthesis of the complementary DNA strand. Rep and Cap are translated to produce multiple distinct proteins...mediate AAV replication. The transfer plasmid, Rep/Cap, and the helper plasmid are transfected into HEK293...gene E1+, to produce infectious AAV particles. Rep/Cap and the adenovirus helper genes may also be combined...into a single plasmid; the separation of Rep and Cap shown in the figure on the right facilitates the ...included the production of synthetic capsids and the mixing of capsids/ITRs from different AAV serotypes ...

...begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus...retroviral particle containing Matrix, Capsid, and Nucleocapsid components. Pol in trans Precursor protein... (Ψ) in cis RNA target site for packaging by Nucleocapsid. WPRE in cis Woodchuck hepatitis virus post‐... of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5kb, but inserts bigger...

...begins, by definition, at the beginning of R, is capped, and proceeds through U5 and the rest of the provirus...lentiviral particle containing Matrix, Capsid, and Nucleocapsid components. Pol in trans Precursor protein...retroviruses (γ-retroviruses) is that lentiviruses are capable of infecting non-dividing and actively dividing... (Ψ) in cis RNA target site for packaging by Nucleocapsid. RRE in cis Rev Response Element; sequence to... of a retroviral provirus (see below). Cloning capacity between the LTRs is ∼8.5kb, but inserts bigger...

...antibodies are capable of recognizing and binding to epitopes on antigens, they are also capable of recognizing...In a sandwich ELISA, a capture antibody, bound to a surface, is used to capture the protein of interest...also be included. Antibody Applications - Capture Methods Capture methods such as immunoprecipitation (IP...variation of IP uses antibodies to capture protein:DNA complexes. After capture, the DNA is released from the...fragmentation of the DNA. Controls for capture methods Capture methods should use a positive control and...methods. Capture MS validation This method uses peptide identification by proteomics (Capture MS) to validate...Amplification Application Overview Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods...

... others work to increase Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes...by the Feng Zhang lab ) combine the large-scale capacity of transposases with the precise DNA-targeting...programmability for different genomic loci, and the capability to detect multiple targets simultaneously. CRISPR...and biotin tags, or an anti-Cas9 antibody. The CAPTURE system, developed by the Jian Xu lab , utilizes... Cas12a also has more condensed multiplexing capabilities , as it can process multiple gRNAs under a single...carrying an ADAR truncation that retain RNA editing capabilities but are small enough to be packaged in AAV particles...two nuclease domains inactivated. This enzyme is capable of cleaving only one strand of target dsDNA. Off-target...

...two components results in a transgene packaging capacity of >8 Kb. Constructs contain left and right arms...transgenes, the use of pAdEasy-2 can increase the capacity of the adenoviral vector. pAdEasy-2 does not contain... immune evasion, deleted to increase packaging capacity E4 Essential for viral transcription; Supplied...

... by the immune system, antibodies are proteins capable of highly specific binding to other proteins. Antibody-based...conjugated to a signaling molecule, can be used to capture, quantify, and identify proteins of interest. Read...

...replicated using the modified bases and a microscope captures the fluorescent color that is emitted each time...