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We narrowed to 449 results for: ENA

Showing: 201 - 220 of 449 results
  1. Plasmids 101: Control Plasmids

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    Blog Post
    ...Proper controls provide the constant variables that enable the correct interpretation of the effect of the...transfection control measures transfection efficiency and enables observation of any effects of the transfection ...
  2. Plasmids 101: The Promoter Region – Let's Go!

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    Blog Post
    ...expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very strong, also called TDH3 ... expression mRNA Yeast promoter for alcohol dehydrogenase I Repressed by ethanol Full length version is...
  3. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...demonstrated excellent counter-selection which enabled very efficient recombineering.     We were inspired...CRISPR/Cas9 system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia...
  4. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

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    Blog Post
    ...authors use affordable, field-ready, Bluetooth-enabled PCR machines that can even be battery operated,...actively interested in science communication and enabling access to science in developing countries. You...
  5. CRISPR 101: Validating Your Genome Edit

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    Blog Post
    ... or drug selection, assuming your reagents are amenable to this. If delivery isn’t an issue, you may need...M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S, Shkumatava A, Teboul...
  6. Quick Guide to Near-Infrared Fluorescent Proteins

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    Blog Post
    ...produce BV, an additional gene coding for heme oxygenase should be supplied (Baloban et al., 2017).  It...multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol 14, 591-600...
  7. An Integrin Antibody Toolkit from IPI

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    Blog Post
    ... embryonic development, extended-open integrins enable tissue morphogenesis, organ development, and the...integrins demanded higher tensions before they would enable cells to spread, resulting in cells that elongated...
  8. Hot Plasmids: Summer 2024

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    Blog Post
    ...The modular system of linkers and spacers could enable many future variations using nanobodies or scFvs...before vitrification, they can be completely denaturated (left) or complexes may dissociate  (right). ...
  9. Antibodies 101: Validation

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    Blog Post
    ...manipulation. Additionally, not all sample types are amenable to genetic manipulation, nor can all genes be ...antibodies for immunocapture applications and is amenable to high-throughput analyses, however, it requires...
  10. Antibodies 101: Antibody Engineering and Directed Evolution

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    Blog Post
    ...ELISA and cell surface display are common. ELISA enables quantitative measurement of antigen-antibody interactions...mutant libraries on the surface of cells. This enables direct screening via flow cytometry for improved...
  11. A History of Genome Engineering in Popular Culture

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    Blog Post
    ...Although genome editing technologies have brought a renaissance in the field of health sciences, energy, and ...533:420–424 . https://doi.org/10.1038/nature17946 Lenay C (2000) Hugo De Vries: from the theory of intracellular...
  12. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post
    ... al., 2008), based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand...one self-labeling tag and a fluorescent protein) enables applications like multi-color imaging (Figure 2...
  13. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmids. Each destination vector contains GFP, enabling you to select cells with high GFP expression. ...This kit is built for serious multiplexing and enables users to express up to 7 gRNAs. The kit contains...
  14. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...-bonded oxygen. This simple enzymatic reaction enabled conversion between the purine bases (A and G) or...., & Zhang, X. (2021). Glycosylase base editors enable C-to-A and C-to-G base changes. Nature Biotechnology...
Showing: 201 - 220 of 449 results