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  1. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...demonstrated excellent counter-selection which enabled very efficient recombineering.     We were inspired...CRISPR/Cas9 system with lambda red recombineering enables simplified chromosomal gene replacement in Escherichia...

  3. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...produce BV, an additional gene coding for heme oxygenase should be supplied (Baloban et al., 2017).  It...multiplex imaging and optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol 14, 591-600...

  4. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...The modular system of linkers and spacers could enable many future variations using nanobodies or scFvs...before vitrification, they can be completely denaturated (left) or complexes may dissociate  (right). ...

  5. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... embryonic development, extended-open integrins enable tissue morphogenesis, organ development, and the...integrins demanded higher tensions before they would enable cells to spread, resulting in cells that elongated...

  6. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ... or drug selection, assuming your reagents are amenable to this. If delivery isn’t an issue, you may need...M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S, Shkumatava A, Teboul...

  11. A History of Genome Engineering in Popular Culture

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    Blog Post
    ...Although genome editing technologies have brought a renaissance in the field of health sciences, energy, and ...533:420–424 . https://doi.org/10.1038/nature17946 Lenay C (2000) Hugo De Vries: from the theory of intracellular...

  12. Antibodies 101: Validation

    Type
    Blog Post
    ...manipulation. Additionally, not all sample types are amenable to genetic manipulation, nor can all genes be ...antibodies for immunocapture applications and is amenable to high-throughput analyses, however, it requires...

  13. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... al., 2008), based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand...one self-labeling tag and a fluorescent protein) enables applications like multi-color imaging (Figure 2...

  15. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ...plasmids. Each destination vector contains GFP, enabling you to select cells with high GFP expression. ...This kit is built for serious multiplexing and enables users to express up to 7 gRNAs. The kit contains...

  16. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    ...-bonded oxygen. This simple enzymatic reaction enabled conversion between the purine bases (A and G) or...., & Zhang, X. (2021). Glycosylase base editors enable C-to-A and C-to-G base changes. Nature Biotechnology...

  18. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ... studies with clinical endpoints. In the first scenario, robust and efficient generation of iPSCs is the...better suited for these situations. The second scenario requires higher levels of safety and as little...

  19. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...activity.  The field of optogenetics is renowned for enabling precise temporal and spatial control. Optogenetics...M, Valeri J, Dunagin MC, Metzger A, Titus KR, Gilgenast TG, Gong W, Beagan JA, Raj A, Phillips-Cremins...

  20. Deep Dive: qPCR

    Type
    Blog Post
    ... be low, reflecting the presence of primarily denatured (single-stranded) DNA. As the temperature drops...should only be one peak, as everything should be denaturing or annealing at the same temperatures. If there...
Showing: 201 - 220 of 435 results