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We narrowed to 1,052 results for: FER

Showing: 201 - 220 of 1052 results

  1. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ... immune system make different isotypes of antibodies that recognize many different epitopes of the target... and protein G each have different affinities for IgG subtypes from different animals so it’s important...successful next experiment!     References and Resources References Ascoli CA, Aggeler B (2018) Overlooked... cover polyclonal antibodies, one of the many different types of antibodies available (others you’ll encounter...polyclonal antibodies from the serum There are a few different ways the antibodies can be isolated from the serum...by an animal are specific for the immunogen. A different type of purification can be performed to isolate...experiments where antibodies raised in several different species are present. One common example is using...

  2. Using AAV for Neuronal Tracing

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    Blog Post
    ...Castle et al., 2014). Differences between AAV serotypes may be also linked to differences in viral uptake by...these different AAV vectors into a brain region, the AAV particles will infect neurons at different ratios...architecture, in particular the connections between different brain regions. For example, communication between...2015). Directional flow of information between different parts of the brain is mediated via individual ... for degradation. These two pathways also use different cytoskeletal machineries to facilitate transport...while anterograde transport relies on kinesin. Different rates of speed for various forms of retrograde... or have been genetically engineered to have differences in their capsid proteins. For a brief overview...

  3. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ...Your AAVancement could be next!   References and Resources References Braun, C. J., Adames, A. C., Saur...allows for a wide variety of screens, including interference, activation, and knockout. Most CRISPR screens...CRISPR screens can be carried out using a few different methods — in vivo, in vitro, and ex vivo. The .... The go-to in vivo option AAV has become the preferred delivery method for in vivo CRISPR screens. AAV...titers equal to those achieved with lentivirus but offer an improved safety profile, customization, and enhanced...mouse tissues. Safety AAV and lentivirus have different safety profiles that often affect which is chosen... cause bad reactions in mice. Customization AAV offers a level of customization that is unmatched by other...

  4. Antibodies 101: Multiplex Immunofluorescence

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    Blog Post
    ...antibodies are all of different species/isotypes and that each secondary has a different color fluorophore...a comment below. Happy imaging! References and Resources References Andrews, N. P., Boeckman, J. X., ...be familiar with immunofluorescence (IF, often referred to as immunocytochemistry (ICC) when the sample...mixture of secondary antibodies, and image all the different colors in one session – fast and easy! Just make...Trimmer Lab, using recombinant antibodies with different isotypes. (A) Neocortex labeled with anti-pan-...make sure you are using primary antibodies from different species or isotypes, so that the secondary antibodies...to align the images of each labeled target as different color channels and analyze the results. Depending...

  5. Donations from Addgene to Yield Answers for Rare Disease Researchers

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    Blog Post
    ...looking at a bunch of different strategies with CRISPR-Cas9. There are so many different variants and strategies...will help them to understand how different mutations lead to differences in the symptoms experienced by ...competition for researchers working on rare diseases, offering them the chance to win some of the latest tools...with the condition to reveal some “outstanding differences.” They now have some theories on the underlying...had the opportunity to meet a few patients that suffered this disease,” Aguilar said. “Once you have that...Institute and Addgene will make a substantial difference in pushing the work forward. Xeroderma Pigmentosum...lot of those plasmids so that we can try many different things in parallel.” Alternating Hemiplegia of...

  6. CRISPR 101: Cytosine Transversion Editors

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    Blog Post
    ...demonstrated that different deaminase and UNG enzymes had different sequence preferences, which they defined...outcomes of their various CGBEs at different target sequences and in different cell types, available at www....non-target strand.” To avoid confusion, we usually use different terminology when discussing base editors, like...up to 68% at the most favorable target sites. Differential cytosine editing between bacteria and mammalian...sites. But the same strategy in E. coli produced different results. GBEs seemed to follow the same initial...editors Now that several labs had shown that preferential C → G editing was possible, the race was on ...creating BE variants with other Cas9s that recognize different PAMs. Their computational tool for predicting ...

  7. Save Time with Transient Plant Leaf Transformations

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    Blog Post
    ... with a T-DNA (transfer-DNA) region. During infection this T-DNA region is transferred into the plant....track, we switched to luciferases to more easily quantify the effect of the different treatments on transformation...method. Transfer your plant into the dark 16 hours before the transformation. We routinely transfer our plants...the T-DNA region with the gene of interest is transferred into the cells. But for C. roseus, Agrobacterium... lot of time. Don't re-invent the wheel. Test different leaf ages Usually younger leaves work better. ...GUS::tMAS to confirm transformation success. Luciferases are great quantitative reporters and use a substrate...substrate to produce bioluminescence. Luciferases usually have shorter mRNA and protein half-lives then...

  8. Important Considerations in Optogenetics Behavioral Experiments

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    Blog Post
    ...applicable to the rat [6]. Technical differences include the ferrule implants and patch cables. One huge...intracranial self-stimulation (ICSS) and place preference. The lab I work in (the Kreek lab) focuses on...behaviors to test reinforcement are ICSS and place preference.   Historically, ICSS was done by implanting ...nose-poke at the active hole [1, 2].    Place preference/place aversion also models reinforcement but ...halves that are distinguished visually through different patterns and the animal only receives stimulation...). If a circuit is reinforcing, the animal will prefer to spend most of its time in the chamber in which...Comparison of c-fos amongst animals that receive different types of stimulation may help to refine and optimize...

  9. Live and Let Dye: Self-Labeling Protein Tags

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    Blog Post
    ...find lots of different plasmids containing the same protein of interest tagged with different fusion proteins...you the flexibility to choose different colors or ligands for different experiments without having to ...intracellular recordings, switching between different colored ligands at different time points.   Figure 3:...or HaloTag have been shown to perform differently for different ligands (Wilhelm et al., 2021). While ...things up for your next experiments! References and resources References Bottanelli, F., Kromann, E. B., Allgeyer...proteins for different experiments over the years — a green fluorescent protein, a red fluorescent protein... Combining orthogonal self-labeling tags on different proteins of interest (or one self-labeling tag...

  10. Plasmids 101: Using Transposons in the Lab

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    Blog Post
    ... transposition.  Target site: Different transposons insert at different DNA sequences or target sites....has several different applications (mutagenesis screens, transgenic animals, gene transfer). The discovery...specific locations in the genome.   References and Resources References Feschotte C, Pritham EJ (2007) DNA...commonly used in the lab While there are many different types of transposons, DNA transposons are most... vectors.   Let’s take a closer look at three different transposon systems adapted for use as research...Sandoval-Villegas et al., 2021). Sleeping Beauty’s preferred target site for integration is TA dinucleotides...piggyBac, Tol2 has a weak consensus sequence for its preferred target integration site: TNA(C/G)TTATAA(G/C)TNA...

  11. Antibodies 101: Isotypes

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    Blog Post
    ...destroy and remove a pathogen. Different isotypes also appear at different stages of an immune response..... Some isotypes have different versions or subclasses that have minor differences in their heavy chain...new Isotype trading cards!     References and resources References Janeway CA Jr, Travers P, Walport...express receptors that recognize the Fc portion of different antibody isotypes. Binding to complement system...that transport antibodies through cells and into different body compartments, such as into mucus, tears, ...Western blotting.  The five isotypes of antibodies Differences in their heavy chain constant regions result ... However, when B cells switch to expressing a different isotype, the intervening DNA sequence is deleted...

  12. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ...conjugated to different fluorophores or a panel of primaries that was raised in different species or are...are complex and antibodies may perform differently in different samples and experimental conditions. Be...for IHC, not all IHC experiments are the same. Differences in sample processing, fixation, antigen-retrieval...make sure that you can easily distinguish each different type of antibody and therefore each target protein...unique isotype or species, then you can choose different fluorophore-conjugated secondary antibodies specific...that has been cited thousands of times may be a safer bet than one that has never been used in a publication... and knowledgeable technical support team, is a safer bet than one from a large distributor that may not...

  13. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

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    Blog Post
    ...organism. Output diversity: Different tools provide results to the user in different formats. This can have...development CRISPR, like the miRNA bubble before it, offers an interesting view into how rapid innovation affects...the piñata beating party goers are altogether different. They are filled with blind anticipation. Their.... Many tools have also opted to mix-and-match different scoring algorithms and parameters. This tapestry...data input in order to generate results. While different types of input are more user-friendly than others..., this is of less concern than data output as different input types can be readily converted.  Examples...later or share them. Community exclusivity: Different communities develop software for their own needs...

  14. Hot Plasmids: Fall 2024

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    Blog Post
    ...middle of proteins) and performs well in many different antibody-based applications.  IPI’s recombinant...recommended for use in western blots (Figure 1). If you prefer to produce your own antibody or want to swap out...virus 5 and their use in revealing antigenic differences between human, canine and simian isolates. J ...early embryogenesis (Figure 3). mScarlet-I3 also offered clearer signals due to reduced background signal...systems don’t always work across species due to differences in natural DNA repair pathways and spontaneous...high rate of spontaneous antibiotic resistance interfering with selection. Using an inducible Cas9 expression...mechanosensor called PILATeS, Pulling-Induced Luciferase Association Tension Sensor (Zhong et al., 2024...

  15. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ... genetic code is universal, many different organisms actually prefer certain codons over others for certain... (Mauro and Chappell, 2014). Different rates of translation by different tRNAs, including those that exhibit...There are 64 different codons that encode 20 amino acids and three stop codons, meaning that the same...important? Translation and protein synthesis relies on transfer RNAs (tRNAs). tRNAs bind to and deliver amino ...tRNAs that are expressed may be found at very different levels (Mauro and Chappell, 2014). Due to this...that is based on the codon usage frequency in a reference of highly expressed genes (Sharp and Li, 1987)... companies, such as IDT, Genewiz and GenScript, offer tools to help with these decisions.  So when do ...

  16. Plasmids 101: Control Plasmids

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    Blog Post
    ...multiple times and on different days. Using our example above, we would test different preparations of Plasmid...Plasmid A in different aliquots of cells on several different days. Biological replicates control for ...There are many, many different types of experiments carried out by scientists every day. Although the...the plasmid without the independent variable. Referring back to the experiment associated with Figure ...constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected with the test ... the results because it provides an important reference point when analyzing the specificity of the shRNA...variable should be consistent over time and from different preparations of the purified test plasmid expressing...

  17. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...findability” of the information on the site. Different users have different strategies for finding what they need...the overall user experience of our website, the different website navigation paths, the organization and...homepage and navigational elements help users who prefer to browse and discover items in our catalog or ...resources through our main dropdown menu. For users who prefer to use search, our updated search engine is now...software team tailored Elasticsearch to support the different usage patterns the larger team identified. The...our catalog of materials and services is quite different from the way it searches through our educational...information in our search indices in order to support different kinds of matches: exact words, partial words, ...

  18. Delivery Methods for Generating iPSCs

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    Blog Post
    ...transgenes in iPSC-derived cells can interfere with their differentiation potential. Finally, retroviruses...reprogramming factors and wait for cells to de-differentiate. However it’s difficult, especially as a newbie...reprogramming proteins. Table 1: Key Features of Different Methods for Generating iPSCs. One important note...reactivation of Myc or other oncogenes after differentiation has been linked to tumor formation in iPSC-...drawback is that lentiviral vectors insert in different genetic locations in each transduced cell, so ...a single polycistronic transgene or with four different adenoviruses, each expressing one factor. These...mutagenesis, adenoviral vectors are considered a safer way to express reprogramming factors for therapeutic...

  19. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...components expressed from different plasmid backbones. They noted that backbone differences such as the order,...assess how different lentiviral plasmid backbone elements impact viral titer and to compare different Cas enzymes...Hurt for his expertise and input.  References and resources References Weber, Ernst, et al. “A Modular ...review common uses of MoClo and the kits Addgene offers to kickstart these applications, no matter what...product in an affordable way, researchers test many different combinations of promoters, terminators, solubility... excellent option (Iverson et al., 2016). CIDAR offers a one-pot reaction wherein multi-part assembly ...is at your service (Lee et al., 2015). This kit offers a broad range of parts, including copy number machinery...

  20. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post
    ...this FRET you speak of? Förster Resonance Energy Transfer (FRET) was originally described by Theodor Förster...alternative acronym Fluorescence Resonance Energy Transfer. Unlike the typical excitation and emission of...excited fluorophore, FRET involves a non-radiative transfer of energy (i.e. no emitted photons) from the excited...excitation by absorption of a photon 2. Energy transfer from the excited donor directly to the acceptor...the acceptor fluorophore. Otherwise the energy transferred from the donor will not be able to excite the...other and be oriented appropriately for energy transfer via dipole-dipole interaction. The efficiency ... typical FRET range, FRET efficiency is used to infer an interaction between the fluorophores and serves...
Showing: 201 - 220 of 1052 results