...premises we can classify a plasmid preparation in 3 different ways: Transformation grade DNA Cloning grade ... our Plasmids 101 blog posts Learn about the differences between transformation, transduction, conjugation...Resources on Addgene.org Read the molecular biology reference Find plasmids for your research Brush up on molecular...

.... This is why they are called transferable skills. Why are transferable skills so important? It is important... just technical skills, but also transferable skills, before offering someone their dream job; They want...   What is a transferable skill? Time management. I needed it when balancing a handful of demanding courses...useful anywhere I work in the future; they are transferrable across most, if not all, industries and work...important to identify which transferable skills you possess because any potential employers, collaborators...certainly matters in the job market, it is the transferable skills that create a full package and add an...an extra shine to your work. Knowing your transferrable skills allows you to build on them even more, ...

...researchers learn many transferable skills while working in the lab and by offering advice on areas where...feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this...where you might need some help. Today in our transferable skills guide: Negotiation. As a grad student,...I should negotiate my salary after receiving an offer but even as I did (successfully!), the process felt...how to spend my grant money (Can I attend this conference?) and with my lab mates on which lab jobs needed...done (Who is responsible for making the running buffer this week?). Scientists negotiate all the time ...Hawaii?). So how can you approach improving this transferable skill? Negotiation as a zero sum game Some ...

...codon-optimized Firefly luciferase genes (e.g., luc2), and NanoLuc® Luciferase are available from the ... creation of NanoLuc® Luciferase from a rather dull 19kDa Oplophorus luciferase by Promega’s Advanced ...than our more common luciferases like firefly (FLuc) and Renilla (RLuc) luciferase. This is important not...or firefly luciferase into mouse livers followed by i.v. injection of furimazine or luciferin 24 hours ...coupled luciferase. This strategy was first applied using RLuc8, an engineered Renilla luciferase, to produce...products at Promega.     References 1. Hall, M.P., et al. (2012) Engineered luciferase reporter from a deep...isn’t science better when we share? NanoLuc® Luciferase-based tools are fantastic examples of technologies...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...Chiara, the tech transfer expert who understands the somewhat complicated Material Transfer Agreement process...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...such an association, start one! These teams can offer a sense of solidarity you’re going to crave. The... - with the possible exception of when Thomas Jefferson dined alone.” There was a time in human history...could know a little bit about everything or in Jefferson’s case, even a lot about everything. Fast forward... a few dozen lab meetings. The lab, as a team, suffered for it. Nobody knew what anyone else was doing...

...Blog Transferable Skills Guide: Problem-Solving Transferable Skills Guide: Teamwork Transferable Skills...recognize their MANY transferable skills learned while working in the lab and offering advice on areas where...the lab can be applied anywhere! References and Resources References Grant, A. (2017). Originals how non-conformists...feel they lack formal training in a variety of transferable skills. At Addgene we're helping to fill this...Today, we’re highlighting an oft-underlooked transferable skill: creativity. I’ve heard quite a few scientists..., it’s clear that creativity is absolutely a transferable skill developed during PhD training. Creativity...inclusivity. Work with and value people who think differently from you and you will arrive at unique solutions...

...hirtus) 630 64 Luciferin ATP No Common uses for plasmids expressing luciferase Luciferase reporter assay...cloning of firefly luciferase was published in 1985, several studies utilized luciferase as a genetic reporter...Well-established Biological sources of luciferase Although luciferase isolated from the firefly beetle (Photinus...bioluminescent reporter, other luciferases have been identified in various species with differing kinetics, substrate...second most commonly used luciferase. Other organisms from which luciferases have been cloned from include...562 64 Luciferin ATP No Jamaican click beetle(Pyrophorus plagiophthalamus) 544-593 64 Luciferin ATP No...containing luciferase and the gene of interest permits translation of functional luciferase, until miRNA...

...Before the conference Is the conference you are attending on the larger side? At large conferences, chances...once in different rooms. Before arriving at the conference, plan out your agenda. Some conferences even ... the entire conference, in general). After the conference Review your favorite conference moments on social...present your findings at a conference. But what you get out of a conference depends on what you put into...introduction. During the conference When you meet someone new at a conference, one of the first things... the conference, you can end by asking them to come to your poster or talk. After the conference After...poster session Before the conference If you’re presenting a poster at a conference, be sure to practice beforehand...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...in mind! References 1. Sinche, Melanie, et al. "An evidence-based evaluation of transferrable skills and...Page Read Other Posts in the Transferable Skills Guide Developing Transferable Skills During Science Training...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...where you might need some help. Today in our transferable skills guide: Collaborating with others outside...collaborators from other institutions personally on conference calls and make sure to maintain these relationships... learning from others and meeting people with different mindsets and approaches can truly broaden your...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...test:  my undergrads were different people! More specifically, they had different learning styles. One was...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...where you might need some help. Today in our transferable skills guide: managing a team. You’ve just been...motivates them (keep in mind that it can be very different from what motivates you!), know their unique skills... strengths (you will likely have reports with different levels of experience), find out what their learning...will take responsibility (within reason!).  Try to buffer them from unnecessary pressure “coming from above...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...where you might need some help. Today in our transferable skills guide: Time Mangement for Scientists. ...tasks. If you’re constantly switching between different projects, you might not be able to make any reasonable...components: A mechanism for blocking off time for different tasks. I use google calendar for this and try ...

...also matters what they’re stored in - their buffer. Buffers are made of chemicals, which have an unfortunate...the wrong everything else.      References and Resources References Boston University Flow Core. Fluorochromes... In this post, we’ll touch on antibody storage, buffer considerations, and give just the lightest of nods...standard curve, another quick experiment, or using a different vial and saving the poor, abandoned antibody for...their antibodies out. It's okay, Blugene!    Buffers  You know what temperature to store your antibodies...conjugation reaction to understand if any of your buffer components could affect the reaction. For instance...sodium azide, a common antimicrobial agent added to buffers, can bind to amine groups present in some conjugates...

...actually been a meeting? Different teams frequently prefer to communicate in different ways, so the source ...arbitrate for them. They were different people with some very different opinions, and some of them would...thomas-kilmann-conflict-resolution-model/    References and Resources References Thomas-Kilmann Conflict Model, Management... blog Management for ScientistsTransferable Skills Guide: LeadershipTransferable Skills Guide: Teamwork...without my “neutral” presence to alleviate those differences, they had a hard time maintaining their friendships...the right time. Like many of the posts in our Transferable Skills series, this post is not meant to be ... that you could strengthen. The right set of transferable skills, coupled with your technical skills, ...

...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...feel they lack formal training in a variety of transferable skills. At Addgene, we've set out to fill this...where you might need some help. Today in our transferable skills guide: Problem-Solving. I don’t know about... careers, I always felt that descriptions of transferable skills seemed fuzzy or ill-defined. In this ...resolves the issue.  If this sounds like about seven different skills to you, you are not wrong. Problem-solving...your life. This tactic is a good way to see how transferable these skills really are, while also practicing...Paired with your other skills, both technical and transferable (i.e. teamwork and time management), your ability...

...resources on the Addgene blog Transferable Skills Guide: Time Management Transferable Skills Guide: Conflict ...of being a scientist and an incredibly useful transferable skill. Being able to stand up in front of an...screeching feedback. You’re muted on Zoom. Your video conferencing software freezes and then crashes. You’re muted...remember to breathe!)   Public speaking as a transferable skill The worst thing about doing public speaking...most difficult form, which makes it the perfect transferable skill. Almost every other form of presenting... a clear, informative presentation, speak at conferences, or even just provide quick updates during a ...Conflict Resolution Transferable Skills Guide: Creativity...

... of the conference Addgenies were lucky enough to meet hundreds of the over 11,000 conference registrants...Check Out Our Microbiology Page! The start of the conference After setting up the Addgene booth on Thursday...involved with was one of the highlights of the conference. It was inspiring to see how microbiology research...out many of the posters being presented at the conference and talked to some scientists who stopped by ...from the nearly 4000 posters presented at the conference, it looks like there are plenty more exciting...Engineering Learn how to Network at Scientific Conferences Resources on Addgene.org Check out Our Microbiology...

...Research" conference. You can register for the conference here. The Future of Research conference is organized... insufficient (or no) emphasis on developing transferable skills to enable pursuit of these diverse opportunities...

... only problematic word. How many different things do you simply refer to as a “tube”? We took steps towards... words, they were leaving these meetings with different interpretations of what we discussed and decided...scientists learn how to communicate their work differently based on their audience. How you talk about your...at a poster is going to vary depending on the conference you’re attending and who approaches you. You ...with professionals from other backgrounds at conferences or networking events. And if you’re a wet lab...fellow team member. But, at Addgene, we use seven different types of “plates” (Fig. 1). When you have to tell... they could in fact be interpreted in several different ways depending on the team. When Tony, our corporate...

... attend conferences. Depending upon your discipline, there are different types of conferences that you...organizations that hold large, annual conferences. Gordon conferences offer more focused and discipline-specific...researchers do learn MANY transferable skills while working in the lab and by offering advice on areas where...experiences, and several Gordon conferences are held every year in different locations all over the world...that they lack formal training in a variety of transferable skills. At Addgene we've set out to fill this...were so critical to me landing my own position! Conferences One of the single most important things you can...world. But simply attending the conference is usually not enough. How do you stand out from the hundreds ...

...types in the body from otherwise terminally differentiated cells simply by expressing four proteins Oct4... usually I only get to scratch the surface of different fields. This meeting has given me an opportunity...can often take the back burner at scientific conferences. In my nearly 20-year career in science, I have...