We narrowed to 397 results for: MUT

... into question. Specifically, CRISPR-generated mutants believed to be knockouts can maintain partial function...process is tedious and time consuming, as RNAi mutants with a reduced growth phenotype will require additional...

...(knockout)? Generate a specific mutant allele of a gene (point mutant)? Increase or decrease expression...Furthermore, in cells containing two mutated alleles, each mutated allele may be different owing to the...many different types of edits, including point mutations, base changes, and large insertions or deletions...) Generate specific genomic edits, like point mutations or small tag insertions, by copying from a DNA...Generate targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA) Prime... alternative splicing, and an early frameshift mutation is more likely to result in a non-functional protein...base editing , or prime editing experiments, most mutated alleles will not contain the desired edit. Even...

... most often consist of an enzyme that has been mutated to block its catalytic activity and instead become... later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent binding of the ...

...especially for G-protein-coupled receptors (GPCRs). Mutations in receptor sites can affect the fluorescence ...doi.org/10.1038/s41592-021-01338-6  Grimm, J. B., Muthusamy, A. K., Liang, Y., Brown, T. A., Lemon, W. C.,...

...Recombinases Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55636 pAAV-EF1a-Cre None ...-dependent Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55641 pAAV-Ef1a-fDIO EYFP...Fluorophores Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55650 pAAV-hSyn Con/Fon EYFP...recombinase-dependent: GECI Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 137119 pAAV-EF1a-Con/Fon-...Excitatory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 55645 pAAV-hSyn Con/Fon hChR2...Inhibitory Opsins Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 137148 pAAV-nEF-Con/Fon-Arch3.3...recombinase-dependent: DREADDs Addgene ID Plasmid Logic Sites and Mutations In-Stock Viral Items 177669 pAAV-nEF-Coff/Fon ...

...glyoxysomal, mitochondrial, peroxisomal) Mutation (specific mutation, "active site", "active site loop", or...-type malate dehydrogenase genes and watermelon mutant clones for your course-based undergraduate research...single protein with many available isoforms and mutants provides a shared experience for the research community...of wild-type MDH genes from several species and mutant clones from watermelon have been codon-optimized...interface") ID Plasmid Description Gene/Insert Mutations Additional Resources MDH CUREs Community (Link...

...Promoter activity GUS activity and fluorescence (GFPmut3.1) Gram-negative bacteria Philip Poole 14471 pRU1144...14462 pRU1097 Promoter activity Fluorescence (GFPmut3.1) Gram-negative bacteria Philip Poole 14461 pRU1701...14083 pAKgfplux1 Promoter activity Fluorescence (GFPmut3a) and luminescence (lux operon) Gram-negative bacteria... 14076 pAKgfp1 Promoter activity Fluorescence (GFPmut3a) Gram-negative bacteria Attila Karsi 18855 FLIParaF.Ec... Mattheos Koffas 173481 HC-M ATP Fluorescence (GFPmut2) Escherichia coli Rahul Sarpeshkar 111614 pCdrA-gfpC...pCdrA-gfpC Cyclic di-GMP Fluorescence (GFPmut2) Pseudomonas aeruginosa Tim Tolker-Nielsen 90217 pMV762-Peredox-mCherry...by knocking out genes or introducing specific mutations can reveal important insights into gene function...

... for use in cancer signaling studies, including mutant, knockdown, and overexpression constructs. Each...growth of the cell. p53 p53, the most frequently mutated gene in human cancer, is a transcription factor...Meyerson , and Root Labs containing wild-type and mutant alleles described in the initial Target Accelerator...vectors for in vitro and in vivo research. TP53 Mutagenesis Library : Pooled library from the Hahn and Root...labs for characterization of all possible p53 mutations. Resources New Tool for Lineage Tracing: The ClonTracer...

...Izaurralde 147272 pAc5.1B-lambdaN-HA-DmeIF4Ga-4Ebind-mut-dsRNAres_Q EIF4G His, HA, V5 Ac5 Parkinson's Elisa...17798 pM-ErbB4CTF-PY1 mutant ERBB4 GAL4-BD ALS Marius Sudol 17799 pM-ErbB4CTF-PY3 mutant ERBB4 GAL4-BD ALS...delta-kinase-PY1 mutant ERBB4 GAL4-BD ALS Marius Sudol 17802 pM-ErbB4-delta-kinase-PY3 mutant ERBB4 GAL4-BD...below can be sorted by disease, plasmid type, mutations, and/or tags such as fluorescent protein (FP) ...Disease Parkinson's Disease Others Gene Type Wildtype Mutant Partial Sequence Codon-Optimized gRNA Other Tags... YFP GAL1 ALS Aaron Gitler 29608 426Gal-FUS-RRM Mutant-YFP FUS YFP GAL1 ALS Aaron Gitler 29609 pcDNA 3.2...TMEM106B_Halo_C_allele TMEM106B Halo Frontotemporal dementia in GRN mutation carriers Michael Ward 178174 VAPB_Halo_C_allele...

...pAAV-synP-FLEX-splitTVA-EGFP-B19G Can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham 100798 pAAV-syn-FLEX-splitTVA-EGFP-tTA...AAV together can be used to complement deletion-mutant rabies virus AAV1 Ian Wickersham 100799 pAAV-TREtight-mTagBFP2...the cell population of interest with a deletion-mutant tracing virus that lacks the ability to infect ... To enable monosynaptic spread of this deletion-mutant rabies, the essential G protein is provided to ...2007a. Retrograde neuronal tracing with a deletion-mutant rabies virus. Nat Methods. 4(1):47-9. PMID: 17179932...

... protein component of MMR) mutant to inhibit MMR PE5 — PE3 plus MLH1 mutant to inhibit MMR PEmax — optimized...installing targeted insertions, deletions, and point mutations using a prime editing guide RNA (pegRNA). As with...tools are available: PE2 — introduction of five mutations in RT enzyme PE3 — PE2 plus additional sgRNA PE4...additional nuclear localization signals, and two mutations in Cas9 to enhance nuclease activity; can be used...

...Ectodomain and RBD mutants. SARS-CoV-2 Spike Receptor Binding Domain Deep Mutation Scanning Library Screening...Screening Jesse Bloom Library of Spike protein RBD mutations for comprehensive mapping. SARS-CoV-2 Spike Receptor...Receptor Binding Domain Site-Saturation Mutagenesis Libraries Screening Jesse Bloom Tyler Starr Libraries...Libraries of variant Spike protein RBD mutations for comprehensive mapping. Other Resources Protocols Coronavirus...

...truncated at both the N and C terminus and induces mutation at rates much higher than the parental vectors...generation architecture has been shown to increase mutation induction when using TALENs. The FokI domains ...homodimeric or enhanced heterodimeric (ELD, KKR mutations) FokI domains. 40131 pCAG-T7-TALEN(Sangamo)-FokI-KKR-Destination... has successfully been employed for deletion mutagenesis and gene targeting in human and mouse pluripotent...suitable for the TALEN-based generation of germline mutations in Bombyx mori and Drosophila melanogaster . Zhang...

...-mPCSK9 hAAT-driven, constitutive Expression of mutant (D377Y) murine PCSK9 for studying hypercholesterolemia...-hPCSK9 hAAT-driven, constitutive Expression of mutant (D374Y) murine PCSK9 for studying hypercholesterolemia... microtubule-associated protein tau with P301L mutation. 1 Tsuneya Ikezu 200777 AAV-HAPLN1 hSyn-driven...hSyn-driven, constitutive Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with...

...dominant manner, are associated with known genetic mutations in one of the following genes: SOD1, C9ORF72, ... huntingtin (HTT) gene. An individual with the mutated HTT gene will have huntingtin proteins with abnormally...-onset forms, a fraction of these are due to a mutation in one of three different inherited genes: Presenilin... collection of plasmids expressing wild type or mutant ORFs, gene fragments, and gRNAs for genes linked...

...precise gene editing. These edits can include mutations seen in human patients, protein tags, or loxP/...editing rates. Advances in this area include: Nickase mutant: cuts only one strand of DNA and can be used with...off-target activity due to rationally designed mutations. Researchers have expanded the CRISPR field beyond...to be used together. Researchers have engineered mutant Cas enzymes with alternative PAM sites . Cpf1 ,...

...Bacteria Drosophila Plant Yeast Resources Cas nickase mutants introduce gRNA-targeted single-strand breaks in...created by wild-type Cas enzymes. To use a nickase mutant, you will need two gRNAs that target opposite strands...strategies reduce unwanted off-target effects. Nickase mutants can also be used with a repair template to introduce...

...different types of target sites available, both mutant and wild type. The ability to manipulate the number...Recombinase-mediated cassette exchange (RMCE): When matching but mutually incompatible recognition sites flank both a DNA...experimental results. Attenuated iCre(R297T), a point mutant iCre variant, reduces the number of recombination...

...Firefly Insertion of 5' promoter/enhancer regions. Mutations within the luciferase gene allow for increased...Renilla CMV Mammalian expression Renilla luciferase mutant Rluc8, which has increased stability and light ...luciferases : A set of three increasingly destabilized mutants which serve as sensors of proteome stress. pHLuc...

...loss-of-function mutations in p53 and Lkb1 , as well as homology directed repair-mediated Kras G12D mutations, leading...KRAS , p53 and LKB1 , the top three significantly mutated genes in lung adenocarcinoma. Delivery of a single...