We narrowed to 256 results for: OVA

1. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   Blog Post

   ... tags and biosensors for multiscale imaging. Shcherbakova et al. Nat Commun. 2016 Vladislav Verkhusha ...

2. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   Blog Post

   ...several great options: pLV hUbC-dCas9-T2A-GFP for removable dCas9 expression and pLM-CMV-R-Cre. Selection ...

3. A Guide to Designing a Scientific Poster: Content, Layout, and More

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   Blog Post

   ...information are inserted into larger circles or ovals, that are arranged in some logical pattern across...

4. Engaging with science and society at pgEd

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   Blog Post

   ...in genetic technologies and other biomedical innovations promise an improved understanding of how our ...

5. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   Blog Post

   ...barely heard of these). These single stranded, covalently closed RNAs seem to be conserved in mammals, ...

6. 10 Basic tips for mammalian cell culture

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   Blog Post

   .... 2. Pagliara, Patrizia, et al. "Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis,...

7. Truncated gRNAs for Regulating Gene Expression

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   Blog Post

   ... scientific discovery through the creation of innovative technologies. To date his group has develop several...

8. Transferable Skills: Negotiation

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   Blog Post

   ...strategy to help get unstuck and move closer to an innovative resolution. Creative negotiation can also decrease...

9. Degrading DNA with Cascade-Cas3

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   Blog Post

   ...1477. https://doi.org/10.1038/s41587-019-0310-0 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,...

10. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...working with mice and slightly less red tape for approval processes. Additionally, while both AAV and lentivirus...

11. Common Injection Routes in Mice

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    Blog Post

    ...expertise available in your lab or organization, and approvals from the organization overseeing your work. Good...

12. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post

    ...are Sf9 cells, which are derived from the pupa ovarian tissue of fall armyworms (Spodoptera frugiperda...

13. Plasmids 101: Multicistronic Vectors

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    Blog Post

    ...more time developing the clever experiments and innovative solutions necessary for advancing the field. ...

14. AAVs in Retinal Gene Therapy

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    Blog Post

    ...trial started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A ...

15. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    Blog Post

    ...that BiFC is irreversible — FP fragments become covalently bonded by associating with one another — which...

16. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    Blog Post

    ...proteins. Cross-linking is a chemical reaction that covalently joins two molecules and it is great for preserving...

17. Antibodies 101: Producing Recombinant Antibodies

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    Blog Post

    ...Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (CHO) cells. (L'Abbé et al., 2018).  Recombinant...

18. CRISPR 101: Making a Knock-In Cell Line

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    Blog Post

    ...mutated in cancer cells, specifically in breast and ovarian lines commonly used in lab. It is recommended you...

19. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ...Eenmaa, H., Leijen, Ä., Pedaste, M., Raju, M., Astapova, A., Lukner, H., Kogermann, K., & Sepp, T. (2021...

20. Cpf1: A New Tool for CRISPR Genome Editing

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    Blog Post

    ...Find plasmids from this publication at Addgene. Makarova, Kira S., et al. "An updated evolutionary classification...