We narrowed to 259 results for: OVA

1. Lab Automation at Addgene

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   Blog Post

   ...that Beckman was flexible and willing to make removable parts to fit on the deck of the Biomek. The FXP...

2. A Guide to Designing a Scientific Poster: Content, Layout, and More

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   Blog Post

   ...information are inserted into larger circles or ovals, that are arranged in some logical pattern across...

3. Open Resources and Plasmid Tools For Studying C. elegans

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   ...domains that naturally “associate, self-excise, and covalently ligate their flanking peptides together (Wang...

4. Split Fluorescent Proteins for Studying Protein-Protein Interactions

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   ... tags and biosensors for multiscale imaging. Shcherbakova et al. Nat Commun. 2016 Vladislav Verkhusha ...

5. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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   ...several great options: pLV hUbC-dCas9-T2A-GFP for removable dCas9 expression and pLM-CMV-R-Cre. Selection ...

6. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

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   ...barely heard of these). These single stranded, covalently closed RNAs seem to be conserved in mammals, ...

7. Truncated gRNAs for Regulating Gene Expression

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   Blog Post

   ... scientific discovery through the creation of innovative technologies. To date his group has develop several...

8. 10 Basic tips for mammalian cell culture

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   .... 2. Pagliara, Patrizia, et al. "Ostreopsis cf. ovata induces cytoskeletal disorganization, apoptosis,...

9. Common Injection Routes in Mice

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   ...expertise available in your lab or organization, and approvals from the organization overseeing your work. Good...

10. Production of Virus in Insect Versus Mammalian Cells

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    ...are Sf9 cells, which are derived from the pupa ovarian tissue of fall armyworms (Spodoptera frugiperda...

11. Transferable Skills: Negotiation

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    Blog Post

    ...strategy to help get unstuck and move closer to an innovative resolution. Creative negotiation can also decrease...

12. Degrading DNA with Cascade-Cas3

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    ...1477. https://doi.org/10.1038/s41587-019-0310-0 Makarova, K. S., Wolf, Y. I., Alkhnbashi, O. S., Costa,...

13. The AAVantages of AAV in CRISPR Screens

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    ...working with mice and slightly less red tape for approval processes. Additionally, while both AAV and lentivirus...

14. Plasmids 101: Multicistronic Vectors

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    ...more time developing the clever experiments and innovative solutions necessary for advancing the field. ...

15. AAVs in Retinal Gene Therapy

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    ...trial started, and 10 more years to obtain FDA approval and make history in the form of Luxturna™. A ...

16. Antibodies 101: Producing Recombinant Antibodies

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    ...Embryonic Kidney (HEK) cells and Chinese Hamster Ovary (CHO) cells. (L'Abbé et al., 2018).  Recombinant...

17. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

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    ...that BiFC is irreversible — FP fragments become covalently bonded by associating with one another — which...

18. Negative Can Be Positive: Open AAV Data with Addgene

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    ...Eenmaa, H., Leijen, Ä., Pedaste, M., Raju, M., Astapova, A., Lukner, H., Kogermann, K., & Sepp, T. (2021...

19. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

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    ...proteins. Cross-linking is a chemical reaction that covalently joins two molecules and it is great for preserving...

20. CRISPR 101: Making a Knock-In Cell Line

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    ...mutated in cancer cells, specifically in breast and ovarian lines commonly used in lab. It is recommended you...