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We narrowed to 627 results for: PROC

Showing: 201 - 220 of 627 results

  2. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    ...scientist at Addgene. Cloning can be quite an arduous process. The PCR could fail to produce a product, the transformation...can go wrong! With all the steps in the cloning process, there are also many ways to troubleshoot the cloning...Addgene. In the lab he develops new assays and processes. Outside the lab, he enjoy the outdoor in all ...

  3. Summer SciComm: Preprints

    Type
    Blog Post
    ...These each have different goals, formats, and processes, and they provide different amounts of access ...journal-based peer review is usually a confidential process among a few individuals.  Early-career researchers...errors, with a lack of transparency in most review processes. Preprints and their feedback, in contrast, are...

  4. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...the two strands are ligated, resulting in the reciprocal crossover event. This event can have three general...the stop codon is excised, and gene expression proceeds. One popular lox-stop-lox plasmid is from Tyler... remove the marker after the initial selection process. By floxing the selection marker, Cre can be used...

  5. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    ...Fragmid. Fragmid is a modular vector assembly process designed for a quick, plug-and-play approach to...of the design work out of the plasmid creation process. Each module of Fragmid represents a class of components..., the Fragmid website, which breaks the design process down into four easy steps, provides the real value...

  6. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    ...she took her first job at Addgene as an Order Processing Assistant. After a few months, she left school...values While going through the co-op application process, my advisor encouraged me to keep my values in ...making mistakes, because it is just part of the process. Additional resources on the Addgene blog Browse...

  7. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    ...cell’s inherent homology directed repair (HDR) process following the double strand break CRISPR/Cas9 makes...stem cell will seek out a template for the repair process. By flooding the cell with a plasmid containing...cell imaging to study cellular structures and processes and can lead to the better understanding of cell...

  8. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ... its expression. More specifically, during the process of RNAi, an enzyme dubbed Dicer cuts or “dices”...Phagocata morgani and Phagocata gracillis, the process is a little more complicated. These strains are...RNA inhibits gene expression in planarians." Proceedings of the National Academy of Sciences 100.suppl...

  9. Tips for Technical Support Calls

    Type
    Blog Post
    ...dissertations. Streamline the question asking process Having thought through the above, don’t be afraid... Help Center Get Help with the Addgene Deposit Process Resources on Addgene.org Check Out Our Frequently...Recieve Them Get Help with the Addgene Ordering Process ...

  10. The AAVantages of AAV in CRISPR Screens

    Type
    Blog Post
    ... — in vivo, in vitro, and ex vivo. The general process of these screens is the same: Deliver CRISPR library... mice and slightly less red tape for approval processes. Additionally, while both AAV and lentivirus have... AAV also has psuedotypes. Psuedotyping is the process of making a hybrid AAV, with the genome from one...

  11. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    ...for speeding things up and for standardizing the process. When we started, too much time for this project...NGG -> NCC, CCN -> GGN). This ends the mutation process. If the guide PAM is within the coding region, ...CrispyCrunch CrispyCrunch take you through the whole process from experimental design to analyzing results. ...

  12. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    ...own detailed plasmid maps as part of the deposit process and these can be found in this location as well...depositors. The more info you provide in the deposit process, the more useful your plasmid pages will be to ...Feature Check out Our Walkthrough of the Deposit Process Learn How We Do Quality Control at Addgene Resources...

  14. Deep Dive: Fixing and Permeabilizing for Immunofluorescence

    Type
    Blog Post
    ...or point in time. You want to halt degradative processes while maintaining the structure of your samples...doi.org/10.1007/978-1-4939-8935-5_26  Rolls G (2022) Process of Tissue Fixation & the Nature of Fixatives in...knowledge-pathway/fixation-and-fixatives-1-the-process-of-fixation-and-the-nature-of-fixatives/  Additional...

  15. Hot Plasmids: Fall 2024

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    Blog Post
    ...are essential tools for visualizing biological processes in vivo. However, mScarlet’s slow maturation rate...like the C. elegans embryo, where developmental processes happen within a few hours. In order to use the...addition to the toolkit for visualizing cellular processes in C. elegans: mScarlet3 for applications needing...

  16. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ...!     Figure 1: Flowchart showing the process for using pooled lentiviral libraries. A) Amplification...of your pooled library during the amplification process. It’s far better to verify via NGS now than to ...want to consider spinfection. Spinfection is the process of transducing cells at a concentrated seeding ...

  17. An Inside Look into Addgene's Dev Team

    Type
    Blog Post
    ...depositor, to streamlining the order fulfillment process to reduce viral order fulfilment from an average... software development team and the development process at Addgene. Diversity supports our innovation ...discussion.  One example of agility at work is our process for working on a new feature. We start with a story...

  18. How to Write a Scientific Review Article

    Type
    Blog Post
    ...limited number of hits. So, here, I will share my process with you. But before I do that, let me say that...really rough and so editing is going to be a long process. I was lucky in that my adviser always played an... and start telling the story. Like any editing process, you will need time away from the article to be...

  19. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    ...arise very quickly following the tried and true process of evolution. Watch this video showing bacteria...targets an essential, conserved, non-negotiable process within the bacterial cell, antibiotic resistance... the development of resistance; it’s a natural process of life and one which has allowed bacteria to evolve...

  20. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    ...’ll choose to study Crepidula atrasolea or Prochlorococcus with your next project. Gymnotiformes and Mormyridae...evolution in immunology, neurobiology, and more. Prochlorococcus (Penny Chisholm) This cyanobacterium is the...need to grow to get a recombinant because this process is expensive. They are using a cool fluorescent...
Showing: 201 - 220 of 627 results