We narrowed to 878 results for: SOM

...overview of some of the common challenges associated with producing and using lentivirus and offer some tips ...(i.e. polyethyleneimine, calcium phosphate or liposomal methods), the volume of the reaction, and the ...transfection ratios can affect transfection efficiency. For some transgenes, transfection optimization simply is ...large inserts; a study by Kumar et al. found that in some cases, titers can drop 10-fold for every 2kb increase...vessel, such as a 3 or 5-layer flask will require some initial optimization; cell number, media volume,... volumes accordingly. While you may need to make some final adjustments, this is generally a good place...

...by offering advice on areas where you might need some help. Today in our transferable skills guide: managing...being a manager: yes, being a manager means being someone’s boss. But it does not mean shouting orders and... opportunities. I still need to check myself sometimes to make sure I am delegating well, but it does...the person in front of me saying “I’ve been doing some of X’s work for a couple of weeks now”. I had no...throughout the year and in a timely manner. If someone’s performance or behavior is inadequate, you need...equally as important so don’t forget to recognize someone’s hard work and reward a job well done. Your report...to your manager. This is when having a mentor - someone more experienced to guide you - is valuable. Protect...

...experience taught me something about myself and my future career. If it wasn’t something I liked – well, I... helped prepare me to start CINSO. Here, I share some of the tips I learned along the way and hope they... favorite. This seemed like a great way to share some of the research taking place at our university with...meet people with similar interests! Especially as someone interested in doing science outreach, not only ...through developing and leading a budding nonprofit, something I had zero experience with before founding CINSO...molecular biology.      Additional Resources I asked some colleagues to share valuable life lessons they have...

...to make something the cell doesn’t need to make. Eukaryotes and some bacteria deploy proteosomes to degrade...and its related fluorescent proteins, which are sometimes used as tags for detection; however, those are...proteins. Tags for stability and solubility What are some of the hurdles to overcome in order to overexpress...there are a number of chemical and peptide-based proteosome inhibitors, glutathione S-transferase (GST), ...assess their function. More detailed information and some protocols can be found in the references provided...

...understand why some people develop the disease while others never do, but we do know that there are some genetic...disease. However, there are some emerging treatments that have shown some positive potential for treating...the first exposure to asbestos to the time when someone starts seeing – or feeling – the first mesothelioma...years, but it usually ranges from 20-50 years and sometimes longer. There is no known safe level of exposure...destruction of cancer cells. Pembrolizumab has shown some great early success in treating mesothelioma, such...

...among organisms aren’t completely understood, but some possible reasons include: Metabolic pressures ...protein may be translated at different rates. The ribosome will move quickly along regions calling for abundant... low abundance, poorly charged tRNAs. When the ribosome stalls, this may give the swiftly translated regions...mismatch between human and E. coli codon preference. Some codons commonly used in humans are not at all common...vice versa. When translating these codons, the ribosome may therefore stall at inappropriate locations...optimized for a particular organism, this will sometimes (but not always) be noted in in the “mutation”...problems surrounding codon choice, you should have some method to make sure the proteins you produce function...

...of inappropriate statistical methods, omission of some data, etc.  The firestorm that followed implementation...who wanted more time to mine their own data, with some smaller labs feeling that larger, better resourced...of the initial contributors. This is probable to some extent - but that is the price of publication. Who...viewing their data months or years before others. In some cases, typically consortia involving genomic screens...not require interaction with the data authors. In some types of research, raw data requires huge amounts...data of any sort in a form that can be parsed by someone other than the actual author. Instead, selected...should data be archived? Simplifying data access Some of the above mentioned issues could be mitigated...

... only improved efficiency for some edits in unpredictable ways. In some cases, however, combining an evolved...find a brief discussion of some of these strategies in our CRISPR guide. Some examples include: TwinPE...perform any base-to-base conversion in principle, but some changes require multiple steps and enzymes. Prime...your prime editing tools. Here, we’ll briefly cover some major advances in prime editing tools. Scroll down...can be used with any of the PE2-5 approaches, sometimes referred to as PE2max, PE3max, etc. PE6: Specialized.... Although the addition of the RNA pseudoknot sometimes altered epegRNA expression compared to pegRNAs...

...your polycistronic E. coli expression constructs. Some applications of plasmids from this kit include the...Melançon III. "Detection and Quantification of Ribosome Inhibition by Aminoglycoside Antibiotics in Living...Living Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1...same restriction enzymes for cloning. Furthermore, some plasmids contain peptides to facilitate protein ...eukaryotic systems and instead directly engages the ribosome for translational complex assembly. PhD student...wide variety of functions via different fusions. Some applications of PUF protein fusions reported in ...Kendall Morgan’s blog post on the PUF Assembly Kit for some insights on its potential from its creators. We ...

...exclusively on. In this blog post, I will go over some advantages, disadvantages, and examples of how scientists...in which very large fragments of the bacterial chromosome (up to 100 kb) were specifically cut out via ...contain regions of homology with the bacterial chromosome fragments. CRISPR cutting was used to circumvent... the need to PCR amplify the fragments of the chromosome, which was technically challenging. Other homology...enzymes, and no scar sequences between fragments. Some of these products include: GeneArt® Seamless Cloning...particular manufacturer of the kit you will be using. Some of these products may offer advantages that Gibson...Ebenstein Y, Zhu TF. Cas9-Assisted Targeting of CHromosome segments CATCH enables one-step targeted cloning...

...going through some of these top blog posts. We’d also like to use this time to hear some feedback from...effort from the Addgenies, we’re able to feature some recently deposited plasmids every quarter. The “...clones in an experiment. Read this blog post for some positive selection (ex: antibiotic selection and... Goedhart Guest blogger Joachim Goedhart shares some strategies for generating GFP fusion proteins. From...there any topics you particularly like or maybe something you’d like to see more of? Let us know by leaving...

...and Events - Amy discusses some of the programs SITN runs and describes some of the more popular topics...with Vini Mani and Amy Gilson from SITN, we discuss some of the many things you can do start your own science...science communicators. In this chapter we learn about some of their events - science seminars, science cafes... to understand the dynamics of information flow. Some of the topics they've recently covered inlcude CRISPR...your own SciComm organization and to learn about some of SITN's other initiatives, email [email protected]...

...destinies” and also suggested some concrete actions that can be taken now to solve some of the more “tractable...finally feels to me like the postdocs are taking some steps to control their own future in science (see...issues in our science training infrastructure for some years.  He spoke eloquently and vehemently about...same time, he pointed out, academic research has some big problems resulting from past practices and mores...unique way of funding basic research at NEB and got some applause when he talked about how he boycotts publishing...

...colleagues and friends why he would give up,“an excellent—some say “cushy”—tenured faculty position for the grind...reasons to stay and good reasons to go.  Here are some questions and perspectives to consider. What do ...surprisingly more rigorous in industrial settings Tight (sometimes impossible) timelines—others are usually waiting...Better resources available ($)—can outsource a task someone else who can do better, no grant writing Job changes...people) -> How can you make a decision about something like this without sufficient data? Starting to...way to shed light on what paths to pursue. Spend some time really thinking about your motivations and ...

...be integrated into the host genome? Expressed episomally long-term? Expressed short-term? Answering these...achieve different biological outcomes. For example, some viruses integrate into the host genome (lentivirus...non-integrating (adenovirus and AAV) but can still express episomally long-term (AAV). If you perform a lipid-based...transposon system, CRISPR targeted strategy, etc.) and episomal expression will last ~2-7 days at moderate levels...levels. With regard to target cells or organism, some primary cells and differentiated cell types are picky...when it comes to transfection/transduction methods. Some ‘difficult to transfect’ cells lines are amenable...

...Homology Directed Repair Addgenie Chari Cortez explains some of the basic science behind homology directed repair... protein production and gives a quick rundown of some of the strains you can use to express your protein...Wyatt and Dale Ramsden from UNC Chapel Hill explain some of the molecular gymnastics behind DNA repair by...Kamens explains what career coaches do and offers up some career coach suggestions. 8. A Match Made in Heaven... with CRISPR as the cargo. Mary Gearing explains some of the clever ways to successfully combine CRISPR...this post, Addgenie Chari Cortez guides us through some common controls and why you might need them. Happy...

...that motivate his interest in neuroengineering, and some of the optogenetic tools that are still missing.... through optogenetics research Addgene: What are some of the big problems that optogenetic tools can help...human behavior, and maybe we can get a handle on some of these mysteries. Another application is clinical...patients? Over a quarter of a million people have some kind of neural implant already. If we can use light...electricity. If you could selectively let in calcium or some other specific ion that would be interesting. It...Addgene: Do you have any special tips or tricks for someone looking to try these optogenetic tools out for ...

...recombinase-dependent AAV vectors. In this article, we will discuss some of the causes of off-target expression and ways ...This means that all DIO/FLEx AAV vectors will have some level of recombination that can lead to leaky expression...is absent. Additionally, we have experienced that some vectors are much more prone to recombination and...the promoter be thoroughly tested in your cells.  Some promoters, such as CAG are very strong and may require...using DIO/FLEx vectors for off-target expression.  Some classes of neurons transiently express TH during...indicating that there is promoter activity. Additionally, some neurons express low levels of GFAP, which can lead...fair amount of time, and it may be tempting to skip some steps and get to experiments. However, by optimizing...

...Everyone in the room has likely just run in from some other commitment. An announcement of purpose can... will too. Even if you are meeting to discuss something difficult, be excited about the prospect of working...meetings short, informative, and pleasant. Email: Sometimes, the right meeting is no meeting at all. When ...checking in on the action items from last meeting. Use some positive peer pressure to make sure everyone completes... your meeting types (see above). We’ve provided some tips above for what works well at Addgene, but meetings... what worked well and ask the meeting leader for some tips. Before long, you will be running collaborative...

... a common big-picture goal. It is worth doing some some serious pre-work to find a lab that will be a ...go about selecting new hires. This is followed by some perhaps surprising, yet important, factors to consider...members to share short-term goals and chat about awesome science. Important factors in the search for a ...of a perfect "fit". How competitive is the lab? Some labs have only one author plus the PI on any paper...Things seem to fall into place frequently and you sometimes feel confident in your abilities. You have intense...