We narrowed to 29 results for: SOM

...the sample, and then through the sample. However, some of that light won’t make it through the sample because...sample or b) using dyes or stains that add color to some components of the sample but not others. The light...your ocular lens. For example, if you observe something using the 10x objective and your ocular lens is...Looking through a microscope’s eyepiece can take some practice. Try to keep both eyes open and adjust ...microscope you may wish to record your observations. Some microscopes have digital cameras attached to them...

...associated with repeated transient transfection. Some lentiviral vectors deliver mammalian antibiotic ...regularly and replace the cell media. Cell death by some cells in the culture may adversely affect the surviving...selection while the population expands. At this time, some researchers reduce the concentration of the antibiotic...integration events. In other words, if an MOI >1 was used, some cells may have 1 copy of the transgene, while others...

... of the previous section so that it will contain some bacteria. Using a fresh, sterile toothpick, or freshly...bacteria overnight (12-18 hours) at 37 °C. Pro-Tip Some plasmids or bacteria need to be grown at 30 °C instead...often true for large unstable plasmids, which sometimes recombine at 37 °C. Be sure to check this before...

...determine the size of the entire construct or excise some or all of an insert from it. Before beginning your...particularly true if you receive a plasmid from someone in another lab, or dig one out of the freezer and...Although this is never an ideal cloning strategy, sometimes it cannot be avoided. If you do have to do so,...

...specific instructions so be sure to check in with someone familiar with your lab’s instrument before use....common 1.5 mL microfuge tubes used in labs, though some larger models can hold larger conical tubes. Depending...speed ranges can vary, but are typically lower than some other centrifuge types. Floor Centrifuges sit on...

... 37 °C for 12–18 h in a shaking incubator. Note: Some plasmids or strains require growth at 30 °C. If ...by a cloudy haze in the media (Figure 1). Notes: Some protocols require bacteria to be in the log phase...went wrong? Try growing the culture for more time. Some bacterial cultures grow more slowly. Also, bacteria...

...generated at any time. Considerations Before You Start Some cell lines tend not to grow well in sparse/individual...integrated transgene could be lost through recombination. Some researchers choose to maintain cells in selective...Seeding an average of 0.5 cells/well ensures that some wells receive a single cell, while minimizing the...

...rip or shred the agar. Do so by gentle spreading. Some spreaders have a sharp edge that can scrape plates...would manifest as a loud pop often accompanied by some light in the cuvette during the electroporation ...Maxipreps as yield can dramatically plateau and sometimes fall off entirely if the column or reagents are...

...doors. For those with latex allergies, be aware that some gloves may contain latex. Consider nitrile gloves... protect your feet. Pro-Tip If they are able, sometimes scientists work for a long time while standing...

...standard plasmid transformation protocol and offer some tips and tricks to reduce transformation time and...microbiology, molecular biology or cell biology. Learn some of the key strategies of aseptic technique, including...

...the laboratory. Don’t mouth pipette! There may be some times when you may be working with a protocol that...titer prior to starting work in the laboratory. For some biohazardous waste, an autoclave or other method...

...important for other antibiotic resistances. Plate some or all of the transformation onto a 10 cm LB agar...your transformation procedure is working. TIP: Sometimes less is more. Although it may be counter-intuitive...

... an intravenous isomolar contrast agent. In research, iodixanol is used as an isomolar density gradient...

... dissolved (but do not overboil the solution, as some of the buffer will evaporate and thus alter the ...Pro-Tip Remember, if you added EtBr to your gel, add some to the buffer as well. EtBr is positively charged...

...contains the plasmid DNA separated from bacterial chromosomes. Collect the supernatant into a new tube by pipetting...salt from the pellet which can cause problems with some common reactions. Air dry the pellet (can be done...

...or test multiple plasmids to find a suitable one. Some labs have reported better results when the plasmid...acceptable) Baseline removal: all samples will have some small amount of background signal that is most evident...

... select multiple target sequences for each gene. Some sequences will be more effective than others. In... viral infection. However, polybrene is toxic to some cell lines. In these cell lines, substitute protamine...

...sterile loop, toothpick or pipette tip to scrape some of the frozen bacteria off of the top. Do not let...

...manually call the correct base at the position. Sometimes an “N” is the result of an erroneous insertion...

...appropriately sized container to put the reagent in. For some reagents, you might need to weigh it out in the ...