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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 29 results for: SOM

Showing: 1 - 20 of 29 results

  1. Using a Light Microscope Protocol

    Type
    Protocol
    ...the sample, and then through the sample. However, some of that light won’t make it through the sample because...sample or b) using dyes or stains that add color to some components of the sample but not others. The light...your ocular lens. For example, if you observe something using the 10x objective and your ocular lens is...Looking through a microscope’s eyepiece can take some practice. Try to keep both eyes open and adjust ...microscope you may wish to record your observations. Some microscopes have digital cameras attached to them...

  2. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...associated with repeated transient transfection. Some lentiviral vectors deliver mammalian antibiotic ...regularly and replace the cell media. Cell death by some cells in the culture may adversely affect the surviving...selection while the population expands. At this time, some researchers reduce the concentration of the antibiotic...integration events. In other words, if an MOI >1 was used, some cells may have 1 copy of the transgene, while others...

  3. Protocol - How to Streak a Plate

    Type
    Protocol
    ... of the previous section so that it will contain some bacteria. Using a fresh, sterile toothpick, or freshly...bacteria overnight (12-18 hours) at 37 °C. Pro-Tip Some plasmids or bacteria need to be grown at 30 °C instead...often true for large unstable plasmids, which sometimes recombine at 37 °C. Be sure to check this before...

  4. Protocol - How to Perform a Diagnostic Digest

    Type
    Protocol
    ...determine the size of the entire construct or excise some or all of an insert from it. Before beginning your...particularly true if you receive a plasmid from someone in another lab, or dig one out of the freezer and...Although this is never an ideal cloning strategy, sometimes it cannot be avoided. If you do have to do so,...

  5. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ... 37°C for 12-18 hr in a shaking incubator. Note: Some plasmids or strains require growth at 30°C. If so...by a cloudy haze in the media (see right). Notes: Some protocols require bacteria to be in the log phase...went wrong? Try growing the culture for more time. Some bacterial cultures grow more slowly. Also, bacteria...

  6. Centrifugation

    Type
    Protocol
    ...specific instructions so be sure to check in with someone familiar with your lab’s instrument before use....common 1.5 mL microfuge tubes used in labs, though some larger models can hold larger conical tubes. Depending...speed ranges can vary, but are typically lower than some other centrifuge types. Floor Centrifuges sit on...

  7. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...generated at any time. Considerations Before You Start Some cell lines tend not to grow well in sparse/individual...integrated transgene could be lost through recombination. Some researchers choose to maintain cells in selective...Seeding an average of 0.5 cells/well ensures that some wells receive a single cell, while minimizing the...

  8. CRISPR Library Amplification

    Type
    Protocol
    ...rip or shred the agar. Do so by gentle spreading. Some spreaders have a sharp edge that can scrape plates...would manifest as a loud pop often accompanied by some light in the cuvette during the electroporation ...Maxipreps as yield can dramatically plateau and sometimes fall off entirely if the column or reagents are...

  10. Video Library

    Type
    Protocol
    ...standard plasmid transformation protocol and offer some tips and tricks to reduce transformation time and...microbiology, molecular biology or cell biology. Learn some of the key strategies of aseptic technique, including...

  11. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...the laboratory. Don’t mouth pipette! There may be some times when you may be working with a protocol that...titer prior to starting work in the laboratory. For some biohazardous waste, an autoclave or other method...

  12. Protocol - Bacterial Transformation

    Type
    Protocol
    ...important for other antibiotic resistances. Plate some or all of the transformation onto a 10 cm LB agar...your transformation procedure is working. TIP: Sometimes less is more. Although it may be counter-intuitive...

  14. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ... dissolved (but do not overboil the solution, as some of the buffer will evaporate and thus alter the ...Pro-Tip Remember, if you added EtBr to your gel, add some to the buffer as well. EtBr is positively charged...

  15. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...contains the plasmid DNA separated from bacterial chromosomes. Collect the supernatant into a new tube by pipetting...salt from the pellet which can cause problems with some common reactions. Air dry the pellet (can be done...

  16. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...or test multiple plasmids to find a suitable one. Some labs have reported better results when the plasmid...acceptable) Baseline removal: all samples will have some small amount of background signal that is most evident...

  17. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... select multiple target sequences for each gene. Some sequences will be more effective than others. In... viral infection. However, polybrene is toxic to some cell lines. In these cell lines, substitute protamine...

  20. Weighing Reagents Protocol

    Type
    Protocol
    ...appropriately sized container to put the reagent in. For some reagents, you might need to weigh it out in the ...
Showing: 1 - 20 of 29 results