We narrowed to 63 results for: SOM

... a double transgenic animal strategy (PV-2a-Cre; SOM-IRES-Flp). Image from Fenno et al., 2014. INTRSECT...window) Cummings KA, Clem RL. 2020. Prefrontal somatostatin interneurons encode fear memory. Nat Neurosci...

... cargo from the cell surface via endosomes, autophagosomes, lysosomes, and more. Browse or search the ...-p62 Autophagosomes Sequestosome-1 EGFP Noboru Mizushima 38272 pMXs-IP GFP-WIPI-1 Autophagosomes WIPI1...mCherry-Lysosomes-20 Lysosomes Lamp1 mCherry* Michael Davidson 79998 pLAMP1-miRFP703 Lysosomes Lamp1 miRFP703...pCMV-mGold2s-Lysosome-N-20 Lysosomes Lamp1 mGold2s Francois St-Pierre 184669 pLAMP1-miRFP670nano3 Lysosomes Lamp1...sequence YFP Thorben Dammeyer 54520 mCherry-Peroxisomes-2 Peroxisomes Peroximal targeting signal (SKL) mCherry... Dorus Gadella 85063 pmScarlet_peroxisome_C1 Peroxisomes Peroximal targeting signal (SRL) mScarlet Dorus...mCherry Desmosomes Desmoglein2 mCherry Kathleen Green 36989 1105-Desmoglein2-FLAG-eGFP Desmosomes Desmoglein2...

...PSMC3 proteasome (prosome, macropain) 26S subunit, ATPase, 3 MGC8487, TBP1 PSMC4 proteasome (prosome, macropain...PSMD10 proteasome (prosome, macropain) 26S subunit, non-ATPase, 10 dJ889N15.2, p28 PSMD11 proteasome (prosome...PSMD14 proteasome (prosome, macropain) 26S subunit, non-ATPase, 14 PAD1, POH1, rpn11 PSMD2 proteasome (prosome...PSMD3 proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 P58, RPN3, S3 PSMD4 proteasome (prosome...MGC110940, OPN SST somatostatin SMST SSTR1 somatostatin receptor 1 SRIF-2 SSTR2 somatostatin receptor 2 - SSTR5...disulfide isomerase family A, member 2 PDA2, PDI, PDIP, PDIR PDIA3 protein disulfide isomerase family A...CCD41, CD201, EPCR, MGC23024, bA42O4.2 PSMB8 proteasome (prosome, macropain) subunit, beta type, 8 (large ...

...iPSCs or transdifferentiation of a somatic cell to a new type of somatic cell. Sort the table by cell type...body. Multipotent stem cells, such as adult (or somatic) stem cells, can differentiate into multiple cell.... To create iPSCs, fully-differentiated adult somatic cells are returned to a pluripotent state using...they can be directly differentiated into specific somatic stem cells or fully differentiated cell types. ...possible to directly differentiate one differentiated somatic cell into another via transdifferentiation. This...Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Science. 2007 Dec 21;318(5858):1917-20. ...drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Cell Stem Cell. 2008 Sep...

...Johannes Vierock 154948 CaMKII-somBiPOLES-mCerulean CamKII BiPOLES (soma-targeted) mCerulean Constitutive...* Simon Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn BiPOLES (soma-targeted) mCerulean Cre dependent...Gradinaru 107708 pAAV-hSynapsin- soCoChR-GFP Syn CoChR (soma-targeted) GFP Constitutive 9 Edward Boyden 107712...107712 pAAV-hSynapsin-FLEX-soCoChR-GFP Syn CoChR (soma-targeted) GFP Cre dependent 9 Edward Boyden 35505 ...pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST Syn ChrimsonR (soma-targeted) mRuby2 Cre dependent 9 Hillel Adesnik ...pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40 EF1a ChrimsonR (soma-targeted) mRuby2 Cre dependent 9 Christopher Harvey...pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3 CAG ChroME (soma-targeted) mRuby3 Cre dependent 9 Hillel Adesnik ...

...targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in... mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites in a viral vector Hillel ...Adesnik 107708-AAV9 pAAV-hSynapsin- soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single... 107712-AAV9 pAAV-hSynapsin-FLEX-soCoChR-GFP A somatic channelrhodopsin, which enables single cell, single...channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear...GRAB_rDA-mut in neurons Yulong Li 158757-AAV8 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter... GCaMP6f. Edward Boyden 158757-AAV9 pAAV-CAG-SomaGCaMP6f2 Cell body-targeted GCaMP6f, under CAG promoter...

...sequence, some base editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation...will introduce the basic mechanisms of CRISPR and some of the ways the CRISPR components are leveraged ...or inversions with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to...cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double digits. These... currently no defined measure of fidelity, so sometimes they will be referred to as increased fidelity...amino acid residues to reduce off-target editing. Some mutations disrupt interactions between Cas9 and ... which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular species...

...Considerations Mismatch cleavage assay Knockouts Some point mutations Some insertions/deletions Very inefficient ...genome relies on proper gRNA design. While there are some cases where it makes sense to manually design a ...See Addgene's HDR blog post for more details. In some cases, there may be no PAM site close to your desired...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral...using cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA method described... genotypes within the resulting cell population. Some cells may remain wild type due to either (1) a lack...Before choosing a validation method, ask yourself some questions: Does the resulting population need to...

...169256 AAV-hSyn-Soma-jGCaMP8s Syn Soma-GCaMP8s Calcium sensor Marianne Fyhn 169257 AAV-hSyn-Soma-jGCaMP8m Syn... Syn Soma-GCaMP8m Calcium sensor Marianne Fyhn 169258 AAV-hSyn-Soma-jGCaMP8f Syn Soma-GCaMP8f Calcium ...sequencing of the final viral vector preparation. In some cases, in vivo testing of these viral preparations...Inhibitor Optogenetics Ofer Yizhar 154948 CaMKII-somBiPOLES-mCerulean CamKII Bidirectional Optogenetics Simon... Simon Wiegert 154951 hSyn-DIO-somBiPOLES-mCerulean Syn Bidirectional, Cre-dependent Optogenetics Simon...

...Sensors GCaMP8 axon-GCaMP8 soma-GCaMP8 ribo-GCaMP8 GCaMP7 GCaMP6 axon-GCaMP6 soma-GCaMP6 Twitch Yellow Calcium...5, 9, rg* GENIE Project 169258 AAV-hSyn-Soma-jGCaMP8f Syn soma-jGCaMP8f none Constitutive 1, 9, rg* Marianne...1, 9, rg* GENIE Project 169256 AAV-hSyn-Soma-jGCaMP8s Syn soma-jGCaMP8s none Constitutive 1, 9, rg* Marianne...5, 9, rg* GENIE Project 169257 AAV-hSyn-Soma-jGCaMP8m Syn soma-jGCaMP8m none Constitutive 1, 9, rg* Marianne...dependent 8 Karl Deisseroth 158757 AAV-CAG-SomaGCaMP6f2 CAG soma-GCaMP6f2 none Constitutive 8, 9 Edward Boyden...

...LAMP-1 Lysosome 101783 MAP1LC3B-mEGFP AICSDP-25 mEGFP Autophagy-related protein LC3 B Autophagosomes 101784...SLC25A17-mEGFP AICSDP-27 mEGFP Peroxisomal membrane protein PMP34 Peroxisomes 101785 TUBA1B-mTagRFP-T AICSDP...Mitochondria 87424 DSP-mEGFP AICSDP-9 mEGFP Desmoplakin Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin Actin...RAB5A-mEGFP AICSDP-39 mEGFP Ras-related protein Rab-5A Endosomes 107580 AAVS1-mTagRFPT-CAAX AICSDP-42 mTagRFPT ...

...Mizushima Autophagy Autophagosome maturation reporter (pmRFP-LC3) Dissection of the autophagosome maturation process...Cuevas-Velazquez , Jose Dinneny Lysosomal pH Lyso-pHluorin for fluorescent readout of lysosome acidification Optogenetic...7458):295-300. Douglas Kim , GENIE Project Calcium Soma-targeted expression of jGCaMP8f, GCaMP7f, or GCaMP6f... Iino Calcium Red calcium sensor FRCaMPi and SomaFRCaMPi, inverted topology of FRCaMP (Fungus based Red...Red CalModulin/M13-like Peptide) A sensitive soma-localized red fluorescent calcium indicator for in vivo...from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Curr Biol. 2018 Feb 5;28(3):444...Optogenetic acidification of synaptic vesicles and lysosomes. Nat Neurosci. 2015 Nov 9. Christian Rosenmund...

...genetics associated with some of the most common disorders followed by a list of some of the relevant collections...Sclerosis Amyotrophic lateral sclerosis (ALS), sometimes referred to as Lou Gehrig’s disease, is a progressive...These familial cases, usually inherited in an autosomal dominant manner, are associated with known genetic... Disease Huntington’s disease (HD) is a fatal autosomal dominant genetic disorder that causes the progressive...

...repetitive and non-repetitive genomic loci, as well as chromosome painting in living cells. Visualizing a specific...labeled proteins to the given region. For example, chromosome-specific repetitive loci can be efficiently visualized...co-delivering multiple gRNAs that tile the locus. Chromosome painting requires delivery of hundreds of gRNAs...gRNAs with target sites throughout the chromosome. Figure 1: Overview of visualizing genomic loci using...

...addresses", where dCas9 is going to bind on the chromosome. When dCas9 binds to a gene, it blocks the progression...bioRxiv preprint . Detailed information about chromosomal integration of pLC97 can be found in this article...Shearwin, K. E. (2013). One-Step cloning and chromosomal integration of DNA. ACS Synthetic Biology, 2(...dCas9 under a P-tet promoter integrated in the chromosome at phage attachment sites. The levels of the ...

...following table describes the five human isotypes. Some isotypes naturally form multi-antibody units, while...concern for conjugating and signaling molecules, as some buffer components can interfere with the conjugate... Sodium azide, an antimicrobial agent, can block some amines needed for conjugation reactions and should...ubiquitously expressed protein such as GAPDH or tubulin (sometimes referred to as housekeeping genes). This allows...have strong binding between the protein and DNA. Some ChIP protocols use enzyme digestion instead of sonication...antibody using the above process and try again. In some cases, it may be preferable to buy small aliquots...

...of the C. elegans hermaphrodite consists of 959 somatic cells, which makes them attractive for imaging ...and/or developmental timeframes. Here we highlight some of the plasmids and tools available at Addgene for...Gene/Insert PI Don’t see your gene listed? Know of some great plasmid tools for the community that aren'...backbones, and constructs for endogenous tagging. Some examples of useful worm CRISPR resources, along ...

...that recognize various nucleotide triplets. With some trial and error, ZF arrays are able to recognize...average genome are every 500 bp. TAL effectors have some restrictions (for example, the target must start...Christian M, Wang L, Zhang Y, Schmidt C, Baller JA, Somia NV, Bogdanove AJ, Voytas DF. Nucleic Acids Res. ...Pubmed PMID: 21493687 . Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Sander...

...phosphoserine-charged tRNA (AUC) and is brought to the ribosome where it recognizes the UAG amber codon and incorporates...phosphosites. Iterative Synthetically Phosphorylated Isomers (iSPI) The iSPI resource described in Galloway ...#188535 ). Importantly, phosphorylation positional isomers are allocated to different subpools, allowing precise...Protocols Iterative Synthetically Phosphorylated Isomers (iSPI) described in: Gassaway, B. M., Li, J., Rad...

...Plasmodium (malaria), Toxoplasma (toxoplasmosis), Trypanosoma (African sleeping sickness), Cryptosporidium ...CRISPR-dCas9 gene regulation has also been established for some of these parasites. Together, these tools have enabled...and development. This collection brings together some of the plasmids that have been used in these efforts...