Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 14 results for: SOM

Showing: 1 - 14 of 14 results

  1. CRISPR Guide

    Type
    Guide
    ...sequence, some base editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation...will introduce the basic mechanisms of CRISPR and some of the ways the CRISPR components are leveraged ...or inversions with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to...cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double digits. These... currently no defined measure of fidelity, so sometimes they will be referred to as increased fidelity...amino acid residues to reduce off-target editing. Some mutations disrupt interactions between Cas9 and ... which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular species...

  2. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ... of both anterograde (from soma to axon) and retrograde (from axon to soma) transport. Many rAAV vectors...viral replication (E4, E2a and VA). These are sometimes combined within the packaging plasmid. For a summary...restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites...genome at a specific site, the AAVS1 site on human chromosome 19. This integration is mediated by proteins ...typically processed into a double-stranded circular episome which can concatemerize, producing high molecular...molecular weight structures that are maintained extrachromosomally. Random integration of rAAV genomes in non-homologous...majority of rAAV DNA is thought to be maintained in episomes or concatemers. This avoids the effects local ...

  3. Molecular Biology Reference

    Type
    Guide
    ...conclusions that genes encode proteins and reside on chromosomes, which are composed of DNA. These findings culminated...order to isolate a particular gene from human chromosomal DNA, it would be necessary to isolate a sequence...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...carry genes and can replicate independently from chromosomal DNA. Although they can be found in archaea and...relationship with the host cell. Like the bacterial chromosomal DNA, plasmid DNA is replicated upon cell division...DNA from one context to another, such as from a chromosome to a plasmid. These tools are essential to the...fragments can be inserted. When a plasmid exists extrachromosomally in E. coli , it is replicated independently...

  4. Adenovirus Guide

    Type
    Guide
    ...remain as extrachromosomal elements within the nucleus, normally as double-stranded circular episomes. Due ...restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites...be packaged in rAAV vectors. This is the case of some commonly used Cas9 homologs, especially when having...genome and instead are maintained in the cell as episomes. This highly reduces the potential for insertional...nonenveloped viruses from the Adenoviridae family. Episome Genetic element inside the cell that remains as...

  5. Lentiviral Vector Guide

    Type
    Guide
    ...leads to serious disease and occurs in many species. Some common types of lentivirus include Human immunodeficiency...plasmids. Although safer, this system can be more cumbersome to use and result in lower viral titers due to...restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites...Standard HEK293T cells are sufficient, but there are some HEK293T cell lines that have been specifically engineered...necessary to properly utilize lentiviral vectors. Some lentiviral plasmids (mostly third-generation) can...Norberg, S. M., Hinrichs, C., Highfill, S. L., Somerville, R. P., Panch, S. R., Jin, P., & Stroncek, D....

  6. Promoters

    Type
    Guide
    ...encoding ribosomal RNA (rRNA) which is a main component of a cell’s ribosome structure. Ribosomes are the...found in eukaryotic cells that package DNA into nucleosomes. Histone binding prevents the initiation of transcription...are responsible for bringing amino acids to the ribosome when proteins are being synthesized. RNA Polymerase...

  7. Antibody Guide

    Type
    Guide
    ...following table describes the five human isotypes. Some isotypes naturally form multi-antibody units, while...concern for conjugating and signaling molecules, as some buffer components can interfere with the conjugate... Sodium azide, an antimicrobial agent, can block some amines needed for conjugation reactions and should...ubiquitously expressed protein such as GAPDH or tubulin (sometimes referred to as housekeeping genes). This allows...have strong binding between the protein and DNA. Some ChIP protocols use enzyme digestion instead of sonication...antibody using the above process and try again. In some cases, it may be preferable to buy small aliquots...

  8. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites... produce more virus after the initial infection. Some retroviral transfer plasmids that have been deposited... with repeated sequence. U5 in cis Unique 5'; in some newer plasmids, this region is removed in 5’ LTRs...proceeds through U5 and the rest of the provirus. Some newer plasmids use a hybrid 5' LTR with a constitutive...

  9. Guide to Using Pooled Libraries

    Type
    Guide
    ...backbone, but they express or target different genes. Some libraries cover the majority of the genome, while...the depositor’s protocol for the best results. For some libraries, plasmid DNA can be delivered directly...Vector resources . Check out our blog posts featuring some of our most popular libraries! Genome-wide Screening...

  10. Cloning

    Type
    Guide
    ... vectors that has a DNA topoisomerase I fused to the 3’ end. The topoisomerase acts as a ligase that joins...

  11. Plan Your Experiment

    Type
    Guide
    ...and variables for using CRISPR in mammalian cells. Some of the variables include: Species of Cas enzyme ... genotypes within the resulting cell population. Some cells may be wild type due to either (1) a lack ...

  12. Chemogenetics Guide

    Type
    Guide
    ... to plan your chemogenetics experiment, here are some things to consider: Chemogenetic activation or inhibition...with cell-type specific promoters. Table 4 lists some common promoters found in chemogenetic receptor ...

  13. Optogenetics Guide

    Type
    Guide
    ...brief overview of the wild-type opsin of each and some examples of relevant variants. This list is not ...pick both an opsin and a delivery system. Here are some key factors to consider: Optogenetic excitation ...

  14. Science Guides

    Type
    Guide
    ...backbone, but they express or target different genes. Some libraries cover the majority of the genome, while...
Showing: 1 - 14 of 14 results