We narrowed to 15 results for: SOM

...sequence, some base editing systems create a wide spectrum of single-nucleotide variants (somatic hypermutation...will introduce the basic mechanisms of CRISPR and some of the ways the CRISPR components are leveraged ...or inversions with two gRNA sites on the same chromosome. Most multiplex systems enable researchers to...cloning multiple gRNAs into a single plasmid — though some have achieved targeting in the double digits. These... currently no defined measure of fidelity, so sometimes they will be referred to as increased fidelity...amino acid residues to reduce off-target editing. Some mutations disrupt interactions between Cas9 and ... which inhibits both Cas12a and Cas3. Similarly, some Acr proteins are specific to a particular species...

... of both anterograde (from soma to axon) and retrograde (from axon to soma) transport. Many rAAV vectors...viral replication (E4, E2a and VA). These are sometimes combined within the packaging plasmid. For a summary...restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites...genome at a specific site, the AAVS1 site on human chromosome 19. This integration is mediated by proteins ...typically processed into a double-stranded circular episome which can concatemerize, producing high molecular...molecular weight structures that are maintained extrachromosomally. Random integration of rAAV genomes in non-homologous...majority of rAAV DNA is thought to be maintained in episomes or concatemers. This avoids the effects local ...

...conclusion that genes encode proteins and reside on chromosomes, which are composed of DNA. These findings culminated...have also expanded the genetic code by redirecting some codons to encode for synthetic amino acids. Plasmids...molecules. To isolate a particular gene from human chromosomal DNA, it would be necessary to isolate a sequence...coined the term plasmid, in reference to any extrachromosomal heritable determinant. Plasmids are fragments...information and can replicate independently from chromosomal DNA. Although they can be found in archaea and...horizontal gene transfer called conjugation. Like chromosomal DNA, plasmid DNA is replicated upon cell division...DNA from one context to another, such as from a chromosome to a plasmid or vector DNA backbone. These tools...

...Considerations Mismatch cleavage assay Knockouts Some point mutations Some insertions/deletions Very inefficient ...genome relies on proper gRNA design. While there are some cases where it makes sense to manually design a ...See Addgene's HDR blog post for more details. In some cases, there may be no PAM site close to your desired...lentiviral preps . AAV vectors AAV vectors offer some advantages for viral vector delivery. Both lentiviral...using cationic lipids, electroporation, or an endosomal approach. Similar to the mRNA method described... genotypes within the resulting cell population. Some cells may remain wild type due to either (1) a lack...Before choosing a validation method, ask yourself some questions: Does the resulting population need to...

...remain as extrachromosomal elements within the nucleus, normally as double-stranded circular episomes. Due ...restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites...be packaged in rAAV vectors. This is the case of some commonly used Cas9 homologs, especially when having...genome and instead are maintained in the cell as episomes. This highly reduces the potential for insertional...nonenveloped viruses from the Adenoviridae family. Episome Genetic element inside the cell that remains as...

...leads to serious disease and occurs in many species. Some common types of lentivirus include Human immunodeficiency...plasmids. Although safer, this system can be more cumbersome to use and result in lower viral titers due to...restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites...Standard HEK293T cells are sufficient, but there are some HEK293T cell lines that have been specifically engineered...necessary to properly utilize lentiviral vectors. Some lentiviral plasmids (mostly third-generation) can...Norberg, S. M., Hinrichs, C., Highfill, S. L., Somerville, R. P., Panch, S. R., Jin, P., & Stroncek, D....

...following table describes the five human isotypes. Some isotypes naturally form multi-antibody units, while...concern for conjugating and signaling molecules, as some buffer components can interfere with the conjugate... Sodium azide, an antimicrobial agent, can block some amines needed for conjugation reactions and should...ubiquitously expressed protein such as GAPDH or tubulin (sometimes referred to as housekeeping genes). This allows...have strong binding between the protein and DNA. Some ChIP protocols use enzyme digestion instead of sonication...antibody using the above process and try again. In some cases, it may be preferable to buy small aliquots...

...encoding ribosomal RNA (rRNA), which is a main component of a cell’s ribosome structure. Ribosomes are the...and histones (proteins that package DNA into nucleosomes) also bind the TATA box. Histone binding prevents...are responsible for bringing amino acids to the ribosome when proteins are being synthesized. RNA Polymerase...

...restriction enzyme, Gibson Assembly, or Gateway. Some transfer plasmids may have limited restriction sites... produce more virus after the initial infection. Some retroviral transfer plasmids that have been deposited... with repeated sequence. U5 in cis Unique 5'; in some newer plasmids, this region is removed in 5’ LTRs...proceeds through U5 and the rest of the provirus. Some newer plasmids use a hybrid 5' LTR with a constitutive...

...backbone, but they express or target different genes. Some libraries cover the majority of the genome, while...the depositor’s protocol for the best results. For some libraries, plasmid DNA can be delivered directly...Vector resources . Check out our blog posts featuring some of our most popular libraries! Genome-wide Screening...

... DNA topoisomerase I covalently bound to the phosphate group on the free 3' T. The topoisomerase acts .... Figure 3: Summary of TOPO cloning. TOPO = topoisomerase I. Created with BioRender.com. Gibson Assembly...

...planning your chemogenetics experiment, here are some things to consider: Activation or Inhibition? Do...with cell-type specific promoters. Table 4 lists some common promoters found in chemogenetic receptor ...

...brief overview of the wild-type opsin of each and some examples of relevant variants. This list is not ...pick both an opsin and a delivery system. Here are some key factors to consider: Excitation or inhibition...

...backbone, but they express or target different genes. Some libraries cover the majority of the genome, while...

...constructs for integration into Y. lipolytica chromosomal loci. Yeast GPCR-sensor Toolkit Yeast Expression...