...developed a machine-learning model to help them construct “designer” channelrhodopsins with desired characteristics...

...on building upon and depositing their modified constructs to Addgene. Congratulations Terry Hébert! ...

... depicted here. (A) Schematic diagram of the construction of the dual virus and the experimental approach...

...Armbruster et al., 2007).  We initially used a construct in which the hM4Di was fused to mCherry. To investigate...

...was rapidly able to support sharing of CRISPR constructs and information across the globe.  “Read the ...

...organisms. Unlike chemical indicators, these DNA constructs can be delivered to intact tissues and organs...

...integrated by the virus. If an oncogene overexpression construct is being delivered, that is a higher risk cargo...

...signaling pathways mediated by GPCRs? The Kruse Lab constructed plasmids that can be used in peroxidase-catalyzed...

...dsDNA construct flanked by ITRs. 293 or 911 cells are transfected with the linearized construct, and virus...vector construction Science... Science Guides Adenoviral Guide Adenoviral Guide Components Vector Construction Frequently...results in a transgene packaging capacity of >8 Kb. Constructs contain left and right arms to facilitate homologous...Plasmid System AdEasy™ for Adenoviral Vector Construction AdEasy™, developed by Bert Vogelstein , is by...popular method for creating adenoviral vector constructs. The system consists of two types of plasmids...linearized with the restriction enzyme PmeI. This construct is then transformed into AdEasier-1 cells , which...

...detailed information on cloning procedures used to construct the deposited plasmids. Molecules: We synthesized...

...modifications for proper protein function. Most DNA constructs designed for mammalian expression utilize viral...

...circular plasmid via PCR. Typically, to make these constructs, developmental biologists have to extract RNA...

...at the plasma membrane in living cells using a construct in which TagRFP-T and Dronpa flanked a PKA substrate...

...sequencing to turn mAb clones into expression constructs to be conveniently modified and engineered.   ...

... for cells stably and highly expressing these constructs can be achieved by fluorescence-activated cell...

...catalog containing over 150,000 sequence-verified constructs to share with scientists across the globe. This...

... CRISPR therapy. They engineered a CRISPR-Cas construct targeting the diseased allele specifically. Early-stage...

...terminator may be desired for multi-cistronic constructs where high termination efficiency is necessary...

...Central PMCID: PMC3138296. 2. Sparta DR, et al. Construction of implantable optical fibers for long-term ...

... the ease with which parts can be combined to construct complex networks. This is accomplished through...