We narrowed to 310 results for: construct

1. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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   ... depicted here. (A) Schematic diagram of the construction of the dual virus and the experimental approach...

2. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

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   ...Armbruster et al., 2007).  We initially used a construct in which the hM4Di was fused to mCherry. To investigate...

3. GPCRs: How Do They Work and How Do We Study Them?

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   ...signaling pathways mediated by GPCRs? The Kruse Lab constructed plasmids that can be used in peroxidase-catalyzed...

4. Popular Retroviral Vectors and Their Uses in Scientific Research

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   ...This modular and flexible system can be used to construct complex lentiviruses for modification of mammalian...

5. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

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   ...cells. The lab will use plasmids from Addgene to construct NFIX expression vectors as well as reporters of...

6. Choosing the B(right)est Fluorescent Protein: Photostability

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   ...proteins and do not require dedicated plasmids or constructs. It should be noted that the photostability measurements...

7. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

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   ...developed a machine-learning model to help them construct “designer” channelrhodopsins with desired characteristics...

8. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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   ...on building upon and depositing their modified constructs to Addgene. Congratulations Terry Hébert! ...

9. Viral Vectors 101: Calcium Sensors

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   ...organisms. Unlike chemical indicators, these DNA constructs can be delivered to intact tissues and organs...

10. Viral Vectors 101: Virus Safety

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    ...integrated by the virus. If an oncogene overexpression construct is being delivered, that is a higher risk cargo...

11. Plasmids for Endogenous Gene Tagging in Human Cells

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    ...guidance on how to make your own gene-tagging constructs below. Read on to find out how these plasmids...

12. Synthetic Biology - Assembly Standards Guide

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    ... the ease with which parts can be combined to construct complex networks. This is accomplished through...

13. Adenovirus Plasmids

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    ...about: Adenoviral components Adenoviral plasmid construction Frequently asked questions about adenovirus ...

14. Retrovirus Plasmids

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    ...cytoplasm Hahn 11375 MDH1-PGK-GFP_2.0 MSCV Retroviral construct for miRNA expression Chen 52114 pMSCV-IRES-mCherry...

15. A Look at Addgene's QC Process

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    ...catalog containing over 150,000 sequence-verified constructs to share with scientists across the globe. This...

16. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

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    ... for cells stably and highly expressing these constructs can be achieved by fluorescence-activated cell...

17. dTAG - You're it!

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    ...detailed information on cloning procedures used to construct the deposited plasmids. Molecules: We synthesized...

18. Plasmids 101: Protein Expression

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    ...modifications for proper protein function. Most DNA constructs designed for mammalian expression utilize viral...

19. Educational Planarian Tools for Teaching Developmental Biology Techniques

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    ...circular plasmid via PCR. Typically, to make these constructs, developmental biologists have to extract RNA...

20. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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    ...at the plasma membrane in living cells using a construct in which TagRFP-T and Dronpa flanked a PKA substrate...