...sequence. Here at Addgene, we use NGS-based quality control to confirm the sequence of all the plasmids we ...ladder based on the expected band sizes. Always run control uncut DNA to ensure your enzymes are working. When...

...recombinase, and Kras G12D HDR template 60225 : control for AAV-KPL; sgRNA targeted to LacZ, plus luciferase...within the mouse genome. This plasmid is used as a control for AAV-KPL. #60226 - AAV:ITR-U6-sgRNA(backbone...targeting the neuronal-specific gene NeuN. As a control they designed an sgRNA targeting LacZ, which is...

...specific small molecules, allowing a scientist to control signaling within cells expressing these engineered...

...rhamnose- and arabinose-inducible promoters. Tight control of toxin expression is absolutely necessary, because...Proteobacteria. We placed the toxin genes under control of a rhamnose-inducible promoter, though Khetrapal...Channarasappa, and Howard M. Salis. "Translation rate is controlled by coupled trade-offs between site accessibility...

...most important ways to spatially and temporally control gene expression, especially in in vivo models, ...functionally similar to Cre-based Brainbow, but is controlled by FLP/FRT recombinase. When placed under different.... The use of AAV provides spatial and temporal control without the need for germline modification, and...

...team develops methods, performs extensive quality control, generates  educational materials, and provides...details about virus production protocols and quality control can be found on Addgene’s Viral Production webpage...

...The gRNAs targeting EGFP can be used as positive controls for gene editing or gene activation, while the...from your primary goal. Getting these quality-controlled reagents from Addgene can greatly expedite a ...

...Ginkgo Bioworks COVID-19 Plasmid Collection Quality control at Addgene in the midst of COVID-19 We received...meant that we had to revamp some of our quality control processes. For the many other COVID-19 related ...

...growth rates will be higher in treatment versus control, and you think there are four important times to..., they will place special emphasis on positive controls and good sample sizes, concerns that are too easy...

...need to ensure you have a bead-only control alongside your input control. Elute the DNA after washing and...

...digest as depicted in Lane 2. Controlling methylation Finally, you can control methylation by altering your...

... These AAV encode tetracycline-inducible tools/controls and tetracycline transactivators that can be used...

... packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF 2.3 MB Zhang GeCKO pooled library...lentiGuide-Puro packaging plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Kits are also available (mouse or human...

...use attP sequence docking sites so that you can control exactly in which locus your transgene ends up (...selection markers include vermillion (another gene controlling eye color) and yellow (a gene affecting the body...

...that expresses gene III. Gene III is under the control of a T7 RNA polymerase and cannot be expressed ...the deaminase TadA-7.10 A mutagenesis plasmid controlling the phage mutation rate Phage infection results...

...lox system with optogenetics. Cre activity is controlled by red light by splitting Cre and fusing each...system, cells express various fluorescent proteins controlled by Cre recombination. Each cell can have multiple...

...communicating the results of the work. Yes, those control experiments are important - but without letting...and finish that experiment (and don’t forget the controls!). And when you’re ready to write up the paper...

...maintained within the yeast cell. These elements control not only the number of plasmids found in each cell... The copy number of these vectors can also be controlled if specific regulatable elements are included...

...Description Mutations Industry PI Due to export control regulations, MERS and SARS-CoV materials are available...

... with different binding affinities or negative-control constructs used to characterize the sensor's behavior... GCaMP6f in PV cortical interneurons under the control of the E2 regulatory element Viral manipulation...in forebrain GABA-ergic interneurons under the control of the mDlx enhancer A viral strategy for targeting...Autophagic Flux Probe that Releases an Internal Control. Mol Cell. 2016 Nov 17;64(4):835-849. Noboru Mizushima...expression of pH sensitive mt-mKeima) Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective...molecular tension sensors reveal extension-based control of vinculin loading. eLife. 2018 Jul 19;7. pii:...