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We narrowed to 303 results for: crispr cas9

Showing: 201 - 220 of 303 results

  1. Call for Guest Bloggers

    Type
    Blog Post
    ...in applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. ...Tips for protein purification Replica plating Anti-CRISPR Retroviral vectors Career development for scientists...

  2. CRISPR Plasmids - dCas9-FokI

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts A...FokI CRISPR: dCas9-FokI Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional...off-target effects of CRISPR. Browse, sort, or search the tables below for CRISPR-FokI plasmids. Plasmids...CRISPR plasmids with dCas9 fused to FokI for creating double-strand breaks (DSBs). CRISPR... CRISPR dCas9-FokI ... A catalytically inactive Cas9 (dCas9) is fused to FokI nuclease. When FokI dimerizes, it generates a ...Editing Other Applications Purify Tag Visualize dCas9-FokI Screen Pooled Libraries gRNAs Empty gRNA Vectors...

  3. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post
    ... using CRISPR/Cas9 to edit a gene, the expression of Cas9 is no longer necessary. However, Cas9 can be... like creating stable cell lines, delivering CRISPR/Cas9 to cells for genome editing, and introducing ...High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology...

  4. CRISPR Plasmids - Mammalian Expression

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Base... to immunoprecipitate FLAG-tagged Cas9. In the CAPTURE system, Cas9 is tagged with a biotin acceptor site... Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of genomic DNA...Publication Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP) can help...CRISPR plasmids that have been designed for use in mammalian systems. CRISPR... CRISPR Mammalian Expression CRISPR...CRISPR Plasmids: Mammalian Expression The following CRISPR plasmids have been designed for use in mammalian...

  5. Editor's Choice, August 2016

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    Blog Post
    ...Viral Transductions Single Base Editing with CRISPR Cas9 Activators: A Practical Guide 10 Steps to a Perfect...technique uses catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions...popular blog post on early user experiences with CRISPR alternative, NgAgo. As you might expect with any...up and running in their labs. Pooran surveyed a CRISPR google forum to get a better idea of how many people...Directed Mutagenesis by PCR & Single Base Editing with CRISPR For my editor’s choice picks this month, I’m picking...Addgene Scientist Mary Gearing and discusses a newer, CRISPR-based technique to generate C->T or G->A transitions...Google Forums Round Up: First Impressions of NgAgo CRISPR Between the Genes: How to Experiment with Enhancers...

  6. Custom CRISPR Screens & the Green Listed Software

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    Blog Post
    ...Learn More About CRISPR Find CRISPR Screening Libraries Browse All CRISPR Plasmids ...world class CRISPR labs.  Check Out This Video to See How Green Listed Works at a Glance!  CRISPR knockout... activity and minimize off-target effects of CRISPR-Cas9." Nature biotechnology 34.2 (2016): 184-191. ...PMC5445660. 7. Chari, Raj, et al. "Unraveling CRISPR-Cas9 genome engineering parameters via a library-on-library...Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips for a 1st CRISPR User Resources on Addgene.org...1000 genes you’d like to investigate in your next CRISPR screen. Luckily, the Green Listed software can ...new software tool used to design gRNAs for custom CRISPR screens targeting a (long or short) list of genes...

  7. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

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    Blog Post
    ...retroviral mouse CRISPR knockout library Article contributed by Alycia Bittner CRISPR pooled libraries...method used to introduce the gRNAs into cells. Most CRISPR pooled libraries have been created in lentiviral...by lentivirus, and thus screening with available CRISPR libraries was not possible. Primary murine T cells...Sarah Teichmann and colleagues created a retroviral CRISPR knockout library using gRNAs designed by the Kosuke...Th2) cells from mice that constitutively express Cas9 and screen for genes that affect Th2 differentiation...factors as the screen readout. This retroviral mouse CRISPR pooled library can be used for screening of cells...in cells with few genetic tools. Find the mouse CRISPR knockout library at Addgene. Henriksson et al.,...

  8. Our Most Popular Blog Posts from 2019

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    Blog Post
    ...and Flexibility to CRISPR Editing by Jennifer Tsang Prime editing is a new CRISPR technique from David...more control and flexibility to CRISPR editing. Using a fusion between Cas9 and reverse transcriptase and...on various topics in biology over the past year: CRISPR, optogenetics, chemogenetics, plasmids, fluorescent... the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, ... recent plasmid blog posts Find the most recent CRISPR blog posts Resources at Addgene Browse the repository...

  9. Editor's Choice, October 2016

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    Blog Post
    ...Community Engineering the Plant Genome Using CRISPR/Cas9 Healthcare Consulting: A Door to the Business...Click on the links below to catch up on ways to use CRISPR in plants, to learn techniques for detecting genome...Posts from October 2016: Sequencing Options for CRISPR Genotyping Grad School Advice Part 2: Building ...

  10. Brzezinski Lab CRISPR Collection

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    ...for gene activation CRISPR/Cas9 Plasmid Variants Figure 1: Schematic of the CRISPR Collection. Plasmids...Collections Brzezinski Lab CRISPR Collection Brzezinski Lab CRISPR Collection The Brzezinski lab investigates...involved in retinal development the lab utilizes a CRISPR/Cas9 based approach to knockout, repress, and activate...Find CRISPR plasmids to study the regulatory networks involved in retinal development. Depositor...taken advantage of the Zhang lab all-in-one (guide/Cas9/reporter) pX458 plasmid family. They have made several... plasmids dCas9-KRAB-MeCP2 fusion for gene and non-coding enhancer sequence repression dCas9-VPR fusion...175574 express dCas9-KRAB-MeCP2 for repression. Plasmids 175571 and 175572 express dCas9-VPR for activation...

  11. CRISPR 101: Any Base Transversion Editors

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    Blog Post
    ...Resources on the Addgene Blog CRISPR 101: Cytosine and Adenine Base Editors CRISPR 101: Cytosine Transversion...Addgene.org Browse CRISPR plasmids for base editing Get a broad overview with our CRISPR Guide Check out ...inserted the deaminase into the C-terminal domain of Cas9, which narrowed the editing window and reduced A...site. Like in most other base editor designs, the Cas9 nicks the non-edited strand to encourage the cell...engineering going on in the base editing field! From CRISPR-free approaches to mitochondrial base editing to...out our CRISPR 101 ebook .... gGBE consists only of engineered MPG fused to nCas9. First, MPG removes the G base from DNA to create...

  12. Plasmids 101: Golden Gate Cloning

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    Blog Post
    ...you are using the Golden Gate method to create CRISPR/Cas9 constructs, assemble standard plasmids parts ...efficiently in just a few steps. More recently, CRISPR technology has adapted Golden Gate cloning for ...oligonucleotides specifying a gRNA target sequence into a Cas9-containing plasmid such as pX330. This cloning strategy... Check out Addgene’s restriction cloning guide! CRISPR 101: Multiplex Expression of gRNAs Choosing a Molecular...

  13. CRISPR Plasmids - Plants

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The... CRISPR Plants CRISPR Plasmids: ...following CRISPR plasmids have been designed for use in plants. Cut Fully functional CRISPR/Cas enzymes... Plasmid Promoter Cloning Enzyme Co-expressed Cas9 Cas9 System Selection PI 46968 pICSL01009::AtU6p aU6...CRISPR expression plasmids that have been designed for use in plants. CRISPR...: Plants Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Promoter Selectable Marker PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted single-strand...

  14. CRISPR Plasmids - Cascade-Cas3

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    ...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts Cas3... CRISPR Cas3 CRISPR Plasmids: Cascade-Cas3...Find plasmids for CRISPR-Cas and Cascade for large genomic deletions. CRISPR...Cascade-Cas3 Browse CRISPR Plasmids By Function Genome Editing Cut Base Edit Nick Prime Edit Transcriptional... Cas3 is a unique member of the CRISPR family. After nicking the target DNA, Cas3 initiates single-strand...genomic deletions. Compared to deletions generated by Cas9, large deletions created using Cas3 have fewer off-target...break, either due to a boundary defined by anti-CRISPR proteins or running into other proteins bound to...

  15. CRISPR Plasmids - gRNAs

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    ... CRISPR gRNAs CRISPR Plasmids: gRNAs Browse CRISPR Plasmids By Function Genome...Zebrafish Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The...PAM sequence. Which CRISPR application is this gRNA sequence compatible with? CRISPR knockout experiments...species or PAM binding variant of Cas9. For instance, wild-type SpCas9 must be used with targets that are...increases off-target effect. Which species or variant of Cas9 ( S. pyogenes , S. aureus etc.) was this gRNA sequence...experiments use targeting sequences within exons, whereas CRISPR activation or repression experiments use targets...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...

  16. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    ...you’re getting started with them. CRISPR Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening...publication. Optimizing Donor DNA for Enhanced CRISPR Genome Editing CRISPR has revolutionized how researchers make... an understanding of Cas9 biophysics! Casilio: An Adaptive, Multitasking “CRISPR-OS” In this post, Albert... can use CombiGEM to barcode small libraries of CRISPR gRNAs to use for screening experiments. Get the...and other types of genome edits, but, even with CRISPR, it isn’t always easy to insert a section of DNA... system that uses modular RNA binding proteins, dCas9, and a modified gRNA to direct a variety of customizable...

  17. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    Blog Post
    ...Your gRNA for CRISPR Genome Editing PRIME Editing: Adding Precision and Flexibility to CRISPR Editing Resources...Resources on Addgene.org CRISPR: PRIME Edit Collection The CRISPR Guide Empty gRNA Expression Vectors...2023). This method uses a prime editor (typically a Cas9 H840A nickase fused to a reverse transcriptase) ...Steensel, B. (2021). Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance...Yoon, S., & Kim, H. H. (2019). SpCas9 activity prediction by DeepSpCas9, a deep learning–based model with... and PE5 prime editing strategies) based on a DeepSpCas9 score ranking (Kim et al., 2019). PRIDICT saves...

  18. Hot Plasmids and Viral Preps - September 2021

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    Blog Post
    ...10.1186/s12915-020-00929-7    The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...A new tool for enriching ABE-targeted cells The CRISPR Corner New ready-to-use viral preps   Improved...end-point sequencing assays. These plasmids use “Cas9-mediated adenosine transient reporter for editing..., in addition to puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide. It was designed...involved in DNA damage response and DNA repair.  A new CRISPR pooled library targeting S. pneumoniae D39V from...pneumococcal strains. The BARBEKO sgRNA library uses CRISPR cytosine base editors to disrupt gene function ...

  19. Hot Plasmids: Summer 2025

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    Blog Post
    ... third of the size of Cas9). Figure 2: Comparison of TnpB (A) and Cas9 (B) genome editors. Effector... TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search for smaller...more in our recent blog post on TnpB). Similar to CRISPR-Cas, TnpB is directed to genomic targets using ..., recruited by either the ωRNA (TnpB) or a gRNA (Cas9). Drawings not to scale. Created with BioRender....

  20. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

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    Blog Post
    ...Modifying the CRISPRi system for multidirectional control The CRISPR interference (CRISPRi) model consists...consists of a catalytically dead Cas9 protein (dCas9) that lacks endonuclease activity coupled with a customizable...Addgene: Plasmids for CRISPRi are described here: http://www.addgene.org/crispr/qi/ Plasmids described...Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds." Adam has also created a video to...metabolic biofactories by demonstrating the use of CRISPR-based RNA scaffolds to mimic natural transcriptional...AdamChinFatt     References Qi, L. S. et al. Repurposing CRISPR as an RNA-guided platform for sequence-specific...Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds. Cell, 160(1), 339-350 (2015). PubMed...
Showing: 201 - 220 of 303 results