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  2. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    ... transcription by blocking its initiation. In mammalian cells, robust repression requires targeting dCas9...delivery systems can be found here “CRISPR 101: Mammalian Expression Systems and Delivery Methods”. Validating...

  3. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    ...variants are capable of cleaving genomic DNA in mammalian cells and zebrafish embryos, and they can be used...has been adapted for targeted RNA cleavage in mammalian cells. The REPAIR (RNA Editing for Programmable...

  4. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    ...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...

  5. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...differentiation of other CNS cell types, including microglia, astrocytes, and oligodendrocytes, provided that... antibodies for many CNS-resident cell types (microglia are a notable exception) necessitates such molecular...

  6. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...1053 amino acids) displayed cleavage activity in mammalian cells. SaCas9 produced indels at a similar efficiency...In vivo interrogation of gene function in the mammalian brain using CRISPR-Cas9. Nat Biotechnol 33:102...

  7. Bioinformatics at Addgene

    Type
    Blog Post
    ... creating a robust data pipeline that would be reliable and available for our teams to use, one of our..., and executed code in Jupyter notebooks using familiar libraries such as Pandas, Numpy, and Biopython...

  8. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...populations in the brain. The Allen Institute and the Janelia Research Campus in the USA have used AAV1 and AAV2...of epitope tags for IHC is the availability of reliable primary antibodies for their detection, particularly...

  9. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    ...FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari...Tsherniak A, Kadoch C (2018) Interrogation of Mammalian Protein Complex Structure, Function, and Membership...

  11. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    ...Electronic lab notebooks and researcher's needs The familiar paper-based lab notebook is still a standard approach...information. Does your lab need to meet any specific compliance requirements – e.g. 21 CFR part 11, HIPAA? Answering...

  12. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...Mangement for Scientists. Back when I used to do mammalian cell culture, I would often respond to friends...side note, I’d recommend the book, “Bored and Brilliant” for learning more fantastic reasons to remove...

  13. Tips for Getting a Faculty Position

    Type
    Blog Post
    ...please do not hesitate to email me at snappe@janelia.hhmi.org." You can find his original blog post below...Director of Student and Postdoctoral Programs at the Janelia Research Campus of the Howard Hughes Medical Institute...

  16. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...aExtinction coefficient. bQuantum yield. cDetermined in mammalian cells. dDetermined as effective NIR fluorescence...Brightness of Near-Infrared Fluorescent Proteins in Mammalian Cells. Cell Chem Biol 24, 758-766 e753 (2017)....

  17. Five Popular Model Organisms

    Type
    Blog Post
    ...musculus) Let’s start with the most preferred mammalian model organism of many researchers: the mouse ...Mus musculus). Mice have many advantages as a mammalian model organism for scientists as they have a relatively...

  19. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...in the medium or flavin containing proteins in mammalian cells. Generally, autofluorescence is less of .... "A genetically encoded fluorescent probe in mammalian cells." Journal of the American Chemical Society...

  20. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...facilitate gene expression in bacteria, yeast, mammalian cells, insects, and even Xenopus oocytes. The ...segments of choice into either an E. coli or a mammalian expression vector in a one-pot reaction. Using...
Showing: 201 - 220 of 538 results