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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 17 results for: lias

Showing: 1 - 17 of 17 results

  1. Video Library

    Type
    Protocol
    ...Deletions with CRISPR Generate genomic deletions in mammalian cells lines using CRISPR/Cas9 Genomic Deletions...adeno-associated virus from a preparation produced in mammalian cells AAV Purification by Iodixanol Gradient Ultracentrifugation...culture in the lab. Blog post: 10 Basic tips for mammalian cell culture Aseptic Technique Aseptic technique...differences in research culture between the US and Australia, and advice for current graduate students considering...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...Web resources Addgene’s mammalian RNAi website: www.addgene.org/mammalianrnai The RNAi Consortium (TRC...resistance gene for selection of pLKO.1 plasmid in mammalian cells. sin 3’LTR 3’ Self-inactivating long terminal...’s lab. For more information, visit Addgene’s Mammalian RNAi Tools page. Several other laboratories have...Weinberg lab. For more information, visit Addgene’s Mammalian RNAi Tools page. E.2 Protocol for Producing Lentiviral...

  3. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...General Transfection Introduce plasmid DNA to mammalian cells Lentivirus Production Produce lentivirus...adeno-associated virus from a preparation produced in mammalian cells Watch the Video! AAV Titration by qPCR Using...to Video Transfection Introduce plasmid DNA to mammalian cells to produce antibodies Antibody Purification...

  4. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

    Type
    Protocol
    ...). You might need to express YGOI in cultured mammalian cells. The problem is that the only version of...Using subcloning, you can easily move YGOI into a mammalian expression vector. Design (Choosing enzymes) Many...purpose. Congratulations, you now have YGOI in a mammalian expression vector and can begin your studies. ...

  5. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...beginning or end of a sequencing reaction is often unreliable. Although your sequencing results may indicate... file, you will see that these base calls are unreliable. This is an example of a trace file from a portion...

  6. General Transfection

    Type
    Protocol
    ...Use this protocol to transfect mammalian cells with your plasmid of interest....protocol describes a general method for transfecting mammalian cells using linear polyethylenimine. Transfections...

  7. Kit Free RNA Extraction

    Type
    Protocol
    ...While simple homogenization is effective for most mammalian tissues; more hardy tissues such as bone, or bacteria...

  8. Centrifugation

    Type
    Protocol
    ...instructions so be sure to check in with someone familiar with your lab’s instrument before use. Last Update...

  9. Fluorescence Titering Assay

    Type
    Protocol
    ...biosafety level 2+. Please ensure that you are in compliance with your institution’s biosafety regulations...

  15. AAV ddPCR Titration

    Type
    Protocol
    ... on the insert. Please ensure that you are in compliance with your institution’s biosafety regulations...

  16. CRISPR Library Amplification

    Type
    Protocol
    ... the specific library. This protocol assumes familiarity with standard bacterial transformation and basic...

  17. Lentivirus ddPCR Titration

    Type
    Protocol
    ...biosafety level 2+. Please ensure that you are in compliance with your institution’s biosafety regulations...
Showing: 1 - 17 of 17 results