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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10 results for: lias

Showing: 1 - 10 of 10 results

  1. CRISPR Guide

    Type
    Guide
    ...strategy yields C to A base edits, whereas in mammalian cells, it produces C to G edits. Adenine transversion...recombinase with improved integration efficiencies in mammalian cells. PASTE ( P rogrammable A ddition via S ite-specific...not suitable for all cell types or experiments. Mammalian CRISPR libraries have also been created in AAV...that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available, such as those...examples of activation systems to target genes in mammalian cells include: SunTag system - co-expression of...unique to bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the Feng Zhang ...video reprint: Protocol for genomic deletions in mammalian cell lines Viral service at Addgene Viral plasmids...

  2. Optogenetics Guide

    Type
    Guide
    ... (from Chloromonas oogama ), and SdChR (from Scherffelia dubia ). Synthetic variants have been created...Chloromonas subdivisa CheRiff Channelrhodopsin from Scherffelia dubia Channelrhodopsins: cation channels- chimeras...induction of protein interactions based on Arabidopsis thaliana cryptochrome 2 and CIB1 450 LARIAT Inhibits protein...optically assembled protein architectures in living mammalian cells. LITE Two-hybrid system integrating the ...domain2 of cryptochrome 2 (Cry2) from Arabidopsis thaliana is fused to truncated forms of cytosolic STIM1...

  3. Plan Your Experiment

    Type
    Guide
    ...focus on making single edits using CRISPR/Cas9 in mammalian cells as an example, but many of these principles...common categories of genetic manipulations used in mammalian cells and their corresponding Cas enzymes. In ...KRAB can be more effective than dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase...straightforward method to deliver a CRISPR system into mammalian cells is to directly transfect expression plasmids...Designing and executing prime editing experiments in mammalian cells. Nature Protocols , 17 (11), 2431–2468. ...

  4. Promoters

    Type
    Guide
    ...Constitutive Strong mammalian promoter from human cytomegalovirus EF1a Constituitve Strong mammalian promoter from... Constitutive Strong hybrid mammalian promoter PGK Constitutive Mammalian promoter from phospholycerate...

  5. Molecular Biology Reference

    Type
    Guide
    ... human cells, the promoter will be a human or mammalian promoter sequence. The promoter can also direct...vectors can drive expression in various cell types (mammalian, yeast, bacterial, etc.), depending largely on...with very few errors making it an efficient and reliable sequencing method. Next Generation Sequencing ...

  6. Adeno-associated virus (AAV) Guide

    Type
    Guide
    ...inherited retinal disease, spinal muscular atrophy, hemophilia A and B, and Duchenne muscular dystrophy. Many...the Allen Mouse Brain Connectivity Atlas and the Janelia Campus MouseLight project . rAAV vectors have also...Gorbatyuk, O. S., Velardo, M. J., Peden, C. S., Williams, P., Zolotukhin, S., Reier, P. J., Mandel, R. ...

  7. Chemogenetics Guide

    Type
    Guide
    ... Specificity hSyn1, CaMKIIa Neurons GFAP Glia CD68 Microglia Dlx Interneurons EF1a, CAG General expression...

  8. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...envelope, Phoenix-AMPHO (for the infection of mammalian cells). Using this system, viral vectors are produced...., Macmorland, W., Horlock, C., Matsumoto, S., Williams, S., Smith, K., Price, J., Srivastava, S., Hussain...

  9. Antibody Guide

    Type
    Guide
    ...only produce one variable region. Isotypes The mammalian immune system produces five classes of antibodies...antibodies from companies that are known to produce reliable antibodies. Be aware that one antibody can and...

  10. Adenovirus Guide

    Type
    Guide
    ...excellent vectors for delivering target antigens to mammalian hosts and have been used as vaccines against infectious...
Showing: 1 - 10 of 10 results