We narrowed to 11 results for: lias

... Assembly into acceptor vectors. The Mammalian Toolkit Mammalian Expression Hana El-Samad 253 plasmids...MoClo-YTK . EMMA: Extensible Mammalian Modular Assembly Toolkit Mammalian Expression Yizhi Cai 95 plasmids...generate mammalian expression vectors for transient or stable transfections. PUF Assembly Kit Mammalian Expression...homology domain, for mammalian or E. coli expression. T2A Split/Link TurboID Kit Mammalian Expression Sven ... Kit Bacterial Expression, Insect Expression, Mammalian Expression Stephan Gruber Platform for rapid assembly... Golden Gate-based cloning methods for use in mammalian cells. These can be used with plasmids from the...Sven Eyckerman Plasmids to assemble mammalian expression vectors for proximity labeling experiments. Multiplex...

...strategy yields C to A base edits, whereas in mammalian cells, it produces C to G edits. Adenine transversion...recombinase with improved integration efficiencies in mammalian cells. PASTE ( P rogrammable A ddition via S ite-specific...not suitable for all cell types or experiments. Mammalian CRISPR libraries have also been created in AAV...that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available, such as those...examples of activation systems to target genes in mammalian cells include: SunTag system — co-expression of...unique to bacterial cells and does not occur in mammalian cells. SHERLOCK , developed by the Feng Zhang ...protocols Video protocol for genomic deletions in mammalian cell lines Viral service at Addgene Viral plasmids...

...Constitutive Mammalian Strong promoter from human cytomegalovirus EF1a Constituitve Mammalian Strong promoter...Constitutive Mammalian Promoter from phospholycerate kinase gene U6 Constitutive Mammalian U6 nuclear promoter...Strong promoter from baculovirus CAG Constitutive Mammalian Strong hybrid promoter; contains CMV early enhancer...response element promoter; often optimized for mammalian systems Types of RNA Polymerases Promoters control...

... (from Chloromonas oogama ), and SdChR (from Scherffelia dubia ). Synthetic variants have been created...Chloromonas subdivisa CheRiff Channelrhodopsin from Scherffelia dubia Channelrhodopsins: cation channels- chimeras...induction of protein interactions based on Arabidopsis thaliana cryptochrome 2 and CIB1 450 LARIAT Inhibits protein...optically assembled protein architectures in living mammalian cells. LITE Two-hybrid system integrating the ...domain2 of cryptochrome 2 (Cry2) from Arabidopsis thaliana is fused to truncated forms of cytosolic STIM1.../10.1523/JNEUROSCI.3863-06.2006 PMID: 17035522 Emiliani, V., Entcheva, E., Hedrich, R., Hegemann, P., ...

...focus on making single edits using CRISPR/Cas9 in mammalian cells as an example, but many of these principles...common categories of genetic manipulations used in mammalian cells and their corresponding Cas enzymes. In ...KRAB can be more effective than dCas9 alone for mammalian cell lines Interfere Activate (CRISPRa) Increase...straightforward method to deliver a CRISPR system into mammalian cells is to directly transfect expression plasmids...Designing and executing prime editing experiments in mammalian cells. Nature Protocols , 17 (11), 2431–2468. ...

...vectors can drive expression in various cell types (mammalian, yeast, bacterial, etc.), depending largely on...promoters that can drive expression of short RNAs. Mammalian shRNA Tools Genome Engineering Plasmids Used to...with very few errors, making it an efficient and reliable sequencing method. Figure 3: Summary of Sanger...

...inherited retinal disease, spinal muscular atrophy, hemophilia A and B, and Duchenne muscular dystrophy. Many...the Allen Mouse Brain Connectivity Atlas and the Janelia Campus MouseLight project . rAAV vectors have also...Gorbatyuk, O. S., Velardo, M. J., Peden, C. S., Williams, P., Zolotukhin, S., Reier, P. J., Mandel, R. ...

... Specificity hSyn1, CaMKIIa Neurons GFAP Glia CD68 Microglia Dlx Interneurons EF1a, CAG General expression...

...envelope, Phoenix-AMPHO (for the infection of mammalian cells). Using this system, viral vectors are produced...., Macmorland, W., Horlock, C., Matsumoto, S., Williams, S., Smith, K., Price, J., Srivastava, S., Hussain...

...only produce one variable region. Isotypes The mammalian immune system produces five classes of antibodies...antibodies from companies that are known to produce reliable antibodies. Be aware that one antibody can and...

...excellent vectors for delivering target antigens to mammalian hosts and have been used as vaccines against infectious...