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We narrowed to 74 results for: lias

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Showing: 1 - 20 of 74 results

  1. Mammalian RNAi Tools

    Type
    Collection
    ... Plasmid Collections Mammalian RNAi Mammalian shRNA Tools for RNAi Browse Plasmids Resources...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...mutations in therapeutic settings. As a tool in mammalian cell systems, silencing is achieved through the...

  2. Synthetic Biology - Mammalian

    Type
    Collection
    ...synthetic biology plasmids for use in mammalian systems. Mammalian Plasmids Search the table by keyword...plasmids for use in mammalian systems. Plasmid... Plasmid Collections Synthetic Biology Mammalian Synthetic Biology...Biology: Mammalian SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering...

  3. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... CRISPR Mammalian Expression CRISPR Plasmids: Mammalian Expression Activate...designed for use in mammalian systems. CRISPR...CRISPR plasmids have been designed for use in mammalian expression systems. To find emtpy gRNA plasmids...Expression Vectors page and search the table for "Mammalian". Activate ID Plasmid Gene/Insert Promoter Selectable...

  4. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression...A206K) pmEGFP-1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD...pCAG-HcRed - Mammalian Expression HcRed1-N1 - Mammalian Expression HcRed1-C1 - Mammalian Expression dKatushka...pQC mPlum IX - Mammalian Expression mPlum-N1 - Mammalian Expression mPlum-C1 - Mammalian Expression mPlum-pBAD...pFast-FT-N1 - Mammalian Expression pTRE-Fast-FT - Mammalian Expression pSCMV-H2B-FT-Fast - Mammalian Expression...-N1 - Mammalian Expression pTRE-Medium-FT - Mammalian Expression pSCMV-H2B-FT-Medium - Mammalian Expression...pSlow-FT-N1 - Mammalian Expression pTRE-Slow-FT - Mammalian Expression pSCMV-H2B-FT-Slow - Mammalian Expression...

  5. Genetic Code Expansion

    Type
    Collection
    ...(Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli pBof Mammalian TAG Huiwang...AbK Mammalian TAG Simon Elsaesser 140011 pAS_4xMx PylT_FLAG-Mx PylRS PylRS M. alvus CpK Mammalian TAG ...-aminotyrosine Mammalian Huiwang Ai 154762 pAS_FLAG-Mma PylRS PylRS M. mazei Mammalian TAG Simon Elsaesser...archaeon Mammalian TAG Simon Elsaesser 154764 pAS_FLAG-Mx1201 PylRS PylRS M. alvus Mammalian TAG Simon...acetyl-lysine Mammalian Tao Liu 164081 pCMV-MfPylRS (human-opti) PylRS M. flavescens acetyl-lysine Mammalian Tao...mazei Mammalian TAG Simon Elsaesser 174896 pAS_4xMmaPylT(UUA)_EF1_MmaPylRS PylRS M. mazei Mammalian TAA ...mazei Mammalian TGA Simon Elsaesser 174901 pAS_MmaPylT_EF1_NES-MmaPylRS WT PylRS M. mazei Mammalian TAG ...

  6. Brain Armamentarium

    Type
    Collection
    ...eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFP Gordon Fishell James M. Wilson...eGFP driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_eGFP Gordon Fishell Viviana Gradinaru...driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_dTomato_nlsdTomato Gordon Fishell ...pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic...AiP14825: pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) Expression of SYFP2 (yellow fluorescent...

  7. Arf GTPase Family

    Type
    Collection
    ...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf3 377 181 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf5 381 180 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Mammalian (pEGFP-N3) Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3) GTPase Arfrp1 10139 201 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3) GAP Arfgap2 84364 521 Bacterial (pET), Mammalian (pcDNA3) GAP Arfgap3...Arap3 (CENTD3) 64411 1544 Mammalian (pEGFP-C2) GAP Acap1 (CENTB1) 9744 740 Mammalian (pFLAG-CMV2) GAP Acap2...

  8. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression...or miniTurbo in the mammalian nucleus pSF3-ultraID - Expresses ultraID in mammalian cells with the Tet-...Independent Cloning (LIC) pAG CMV Empty Puro - Empty mammalian expression plasmid for cloning untagged proteins...proteins pcDNA3.1-mCherry - Expresses mCherry tag in mammalian cells pBI-MCS-EGFP - Tet-inducible Find more Tet-inducible...FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for mammalian expression pNIC-CTHF - C-terminal ...including Xenopus HA Epitope tag pcDNA3.1-HA - Mammalian expression vector with N-terminal HA tag pInducer20...Myc Epitope tag pKMyc - N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector ...

  9. Caltech Systemic Capsids

    Type
    Collection
    ...AiP13044 - pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) minBG SYFP2 Control AIBS , Ting 191707 ...AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237) minBG SYFP2 Control AIBS , Ting 191708 ...AiP12700 - pAAV-AiE0452h-minBG-mTFP1-WPRE3-BGHpA (Alias: CN2700) minBG mTFP1 Control AIBS , Ting 191720 ...AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038) minBG SYFP2 Control AIBS , Ting 191726 ...AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) minBG SYFP2 Control AIBS , Ting 191728 ...AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) minBG SYFP2 Control AIBS , Ting 191737 ...AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610) minBG SYFP2 Control AIBS , Ting 199773 ...

  10. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase-pcDNA3 Firefly CMV Mammalian expression of firefly luciferase William Kaelin 60495 pSBtet-GP Firefly... CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression...Gaussia luciferase (Gluc) NanoLuc® (Nluc) The mammalian codon-optimized version of firefly luciferase ...noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s) Description...of a CMV promoter is present for normalization William Kaelin 71248 pXPG Firefly Insertion of 5' promoter... expression of luciferase in the cytoplasm of mammalian cells Peter Cockerill 212936 pGL3 Basic Vector...Firefly Creation of N-terminal Firefly fusions in Drosopholia Michael Rosbash 128046 pGL3basic luciferase and...

  11. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 174039 pU6-tmpknot-GG-acceptor Mammalian hU6 epegRNA...CRISPR Prime Edit CRISPR Plasmids: Prime Edit Mammalian Bacteria Drosophila Plant Empty pegRNA Resources... other CRISPR topics, read our CRISPR Guide . Mammalian ID Plasmid Gene/Insert Promoter Selectable Marker...Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6 pegRNA BsaI No mRFP1 David Liu 140448 QPM-... No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI for sgRNAs; BbsI...sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob ...Giehm Mikkelsen 173222 pPBT-PE2-PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV...

  12. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...pGFP-Rab8A Primary cilia Rab8 EGFP Maxence Nachury 35623 pEGFPN3-Sstr3 Primary cilia Sstr3 EGFP Kirk Mykytyn...pEGFPN3-5ht6 Primary cilia Htr6 EGFP Kirk Mykytyn 104358 pEGFP-N-Drd1 Primary cilia (Neurons) Drd1 EGFP... ensconsin GFP William Bement 26742 EMTB-mCherry Microtubules Ensconsin mCherry William Bement 164460 ...Mitochondrial targeting sequence (COX8) mStayGold Janelia Integrative Imaging 189774 p4xmts-mScarlet3_N1 ...79990 pKeratin-miRFP703 Intermediate filaments (epithelial) Keratin miRFP703 Vladislav Verkhusha 79991 pmiRFP703...-mGold2t-Keratin-N-17 Intermediate filaments (epithelial) Keratin mGold2t Francois St-Pierre 231767 pCMV-mGold2s-Keratin-N...-mGold2s-Keratin-N-17 Intermediate filaments (epithelial) Keratin mGold2s Francois St-Pierre 26737 GFP-UtrCH...

  13. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ... nuclease domains: pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in... domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be used as a substitute...domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802 (for expression of...domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette...domain ( Sollu et al., Nucleic Acids Res ) in mammalian cells or zebrafish). pCLR2068 (for expression ...domain. ( Doyon et al., Nat Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al...

  14. TALEN Plasmids and Kits

    Type
    Collection
    ...Destination constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled TALEN...destination vectors and a reporter vector for mammalian cell-based validation assay that are designed ...vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing unique...Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize...plasmids were optimized for TALEN expression in mammalian systems including hard-to-transfect cell lines...sequence to drive efficient TALEN expression in mammalian cells, (iv) an improved, truncated TALE backbone...and pTAL7b for enrichment of double-transfected mammalian cells. In addition, a T7 promoter was included...

  15. COVID-19 Resources

    Type
    Collection
    ...Return to top Plasmids Encoding Mammalian Genes or Inserts Several mammalian genes have been identified as...SARS-CoV-2 Plasmids Other Coronavirus Plasmids Mammalian Targets General Plasmid Tools Pooled Libraries... SARS-CoV-2 plasmids for E. coli , yeast, and mammalian expression, and spike protein...cell protein 4) is involved in beating of airway cilia (Kogiso et al., 2020) Cytokines - SARS-CoV-2 infection...CRISPR tools, like Cas12a and Cas13a plasmids, to reliably detect nucleic acids SARS-CoV-2 main protease ...., Marunaka, Y., & Nakahari, T. (2020). Airway Ciliary Beating Affected by the Pcp4 Dose-Dependent [Ca2...window) Wang, K., Chen, W., Zhang, Z., Deng, Y., Lian, J. Q., Du, P., Wei, D., Zhang, Y., Sun, X. X., ...

  16. Immunology Research Plasmids and Resources

    Type
    Collection
    ...8 CKLFSF8, CKLFSF8-V2 CNTF ciliary neurotrophic factor HCNTF CNTFR ciliary neurotrophic factor receptor...glucagon-like peptide 2 receptor - GMFB glia maturation factor, beta GMF GMFG glia maturation factor, gamma GMF-...vascular endothelial growth factor A MGC70609, MVCD1, VEGF, VEGF-A, VPF VEGFB vascular endothelial growth...Categories Plasmid Tables Additional Resources Mammalian organisms are exposed to millions of potential...histocompatibility complex, class II, DQ alpha 1 CD, CELIAC1, DQ-A1, FLJ27088, FLJ27328, GSE, HLA-DQA, MGC149527...histocompatibility complex, class II, DQ beta 1 CELIAC1, HLA-DQB, IDDM1 HLA-DRA major histocompatibility...HsT17083, P58, PI-PLC PROCR protein C receptor, endothelial (EPCR) CCCA, CCD41, CD201, EPCR, MGC23024, bA42O4.2...

  17. Tetracycline Inducible Expression

    Type
    Collection
    ...engineered to eliminate binding of endogenous mammalian transcription factors to further minimize background... expression of the transactivator proteins in mammalian cells or other species by codon optimization of...expression of EGFP Tet-On 3G rtTA TRE3GS Xiaojun Lian 171123 pLVX-TetOne-Puro-GFP Lentiviral Tet-On vector...Tet-On 3G rtTA Oskar Laur 96963 pCAG-TetON-3G Mammalian expression of the Tet-On 3G transactivator Tet-On...plasmids. tTA Viviana Gradinaru 104102 pCAG-tTA Mammalian expression of tTA from the CAG promoter for Tet-Off... 172878 pSF3-ultraID Expression of UltraID in mammalian cells with the Tet-On or Tet-Off system Julien...Joan Brugge Return to Top Additional Resources Mammalian shRNA Plasmids Cre-lox Plasmids Stem Cell Research...

  18. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...jGCaMP8 Fast Genetically Encoded Calcium Indicators. Janelia Research Campus. Online resource. 2020 GENIE Project...tethering by PDZD8 regulates Ca 2+ dynamics in mammalian neurons. Science. 2017 Nov 3;358(6363):623-630...Acetyl-CoA PancACe acetyl-CoA sensor for bacterial or mammalian expression, targeted to nucleus, cytoplasm, or... Fluorescent sensor of cyclic AMP (cAMPr) for mammalian or insect expression (Cre-dependent) cAMPr: A ...extracellular L-lactate, in neurons, astrocytes or mammalian cells A genetically encoded fluorescent biosensor...Serotonin Serotonin sensor iSeroSnFR for bacterial or mammalian expression (Constitutive or Cre-dependent) Directed... pii: S0092-8674(21)00374-3. Lin Tian Serotonin Cilia-targeted serotonin sensor GRAB-HTR6 A serotonergic...

  19. Retrovirus Plasmids

    Type
    Collection
    ...expression. See plasmid 8452 for puromycin resistance. William Hahn 63704 pRetroX GFP T2A Cre CMV/MSV Tetracycline...inducible expression of GFP T2A Cre fusion in mammalian cells. Floris Foijer 47916 pRXTN CMV/MSV A modified...element that increases export to the cytoplasm. William Hahn 11375 MDH1-PGK-GFP_2.0 MSCV Retroviral construct...-Luc MoMSV Stable expression of luciferase in mammalian cells. Alice Wong 83356 pMXs-3XHA-EGFP-OMP25 MoMSV... For tagging mitochondria with HA epitopes in mammalian cells. David Sabatini Return to top Popular Gamma-Retroviral...

  20. Antibody Plasmid Collection

    Type
    Collection
    ...into plasmids and expressing these in bacteria, mammalian cells, yeast, plants, or insect cells. Benefits...recombinant monoclonal antibodies (R-mAbs) expressed in mammalian cells. Monoclonal antibodies are derived from ...the plasmids are transiently transfected into mammalian tissue culture cells, the R-mAbs are secreted ...antibody heavy- and light-chain DNAs into a single mammalian expression vector A tool kit for rapid cloning...2014 Jul 30;4:5885. Andrew Beavil Cloning and mammalian expression of mouse and human IgG monoclonals ...
Showing: 1 - 20 of 74 results