Skip to main content
Addgene
Showing: 1 - 20 of 96 results

  1. Mammalian RNAi Tools

    Type
    Collection
    ... Plasmid Collections Mammalian RNAi Mammalian shRNA Tools for RNAi Browse Plasmids Resources...Plasmids for mammalian RNA interference (RNAi) experiments at Addgene; browse by constitutive lentiviral...mutations in therapeutic settings. As a tool in mammalian cell systems, silencing is achieved through the...

  2. Synthetic Biology - Mammalian

    Type
    Collection
    ...synthetic biology plasmids for use in mammalian systems. Mammalian Plasmids Search the table by keyword...plasmids for use in mammalian systems. Plasmid... Plasmid Collections Synthetic Biology Mammalian Synthetic Biology...Biology: Mammalian SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering...

  3. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... CRISPR Mammalian Expression CRISPR Plasmids: Mammalian Expression The... that function in mammalian cells can be used to attentuate RNA levels. In mammalian systems, Cas13a does...or cloning method. To isolate mammalian vectors, simply type "Mammalian" into the search bar. When using...designed for use in mammalian systems. CRISPR...CRISPR plasmids have been designed for use in mammalian expression systems. Cut Fully functional CRISPR...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...

  4. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ...Genomic Deletions in Mammalian Cell Lines CRISPR: Protocol for Genomic Deletions in Mammalian Cell Lines Other...Experiments (JoVE) about genomic deletions in mammalian cell lines using CRISPR/Cas9 CRISPR...of CRISPR/Cas9 to create genomic deletions in mammalian cell lines. Along with the video, you can find...Article Citation Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. Bauer DE, Canver MC...loss-of-function studies of genes and genetic elements in mammalian cell lines. Protocol For referenced Tables and...limiting dilution prior to performing this step to reliably obtain approximately 30 cells per 96-well plate...excerpted from: Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9. Bauer DE, Canver MC...

  5. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...pcDNA3-EGFP - Mammalian Expression pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression...A206K) pmEGFP-1 - Mammalian Expression mEGFP-N1 - Mammalian Expression mEGFP-C1 - Mammalian Expression mEGFP-pBAD...pCAG-DsRed2 - Mammalian Expression DsRed2-N1 - Mammalian Expression DsRed2-C1 - Mammalian Expression DsRed2...pCAG-HcRed - Mammalian Expression HcRed1-N1 - Mammalian Expression HcRed1-C1 - Mammalian Expression dKatushka...pQC mPlum IX - Mammalian Expression mPlum-N1 - Mammalian Expression mPlum-C1 - Mammalian Expression mPlum-pBAD... Sirius-N1 - Mammalian Expression SBFP2 380 446 13 Monomer (A206K) pSBFP2-C1 - Mammalian Expression Azurite...Monomer (A206K) mAzurite-N1 - Mammalian Expression mAzurite-C1 - Mammalian Expression EBFP2 383 448 17 ...

  6. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...gRNA_Cloning Vector 41824 Mammalian AfIII none S. pyogenes Bleocin Church MLM3636 43860 Mammalian BsmBI none S. pyogenes...pSPgRNA 47108 Mammalian BbsI none S. pyogenes Gersbach pUC57-sgRNA expression vector 51132 Mammalian BsaI none...sgExpression 48240 Mammalian BbsI yes, activate S. pyogenes Jaenisch pSQT1313 53370 Mammalian BsmBI none S....Gen) 48140 Mammalian BbsI yes, nick S. pyogenes EGFP Zhang PX460 (3rd Gen) 48873 Mammalian BbsI yes, nick... Gen) 48138 Mammalian BbsI yes, cut S. pyogenes EGFP Zhang PX335 (2nd Gen) 42335 Mammalian BbsI yes, nick...Gen) 42333 Mammalian BbsI yes, nick S. pyogenes Puro Zhang PX330 (2nd Gen) 42230 Mammalian BbsI yes, cut...42229 Mammalian BbsI yes, cut S. pyogenes Puro Zhang sgRNA(MS2) cloning backbone 61424 Mammalian BbsI ...

  7. Cre-lox system

    Type
    Collection
    ...cTnT Mammalian Campbell 89573 pCAG-iCre iCre CAG Mammalian Wong 89575 pCAG-VCre VCre CAG Mammalian Wong... CAG Mammalian Scholer 125574 pCAG-Cre Cre CAG Mammalian Imai 125577 pCAG-iCre iCre CAG Mammalian Imai...p209 pCMV-cre-K Cre-K CMV Mammalian Green 8395 p210 pCMV-CREM CREM CMV Mammalian Green 8401 p224 pCMV-RFP...floxed RFP within CREM CMV Mammalian Green 8403 p153 pPr-cre Cre probasin Mammalian Green 8404 p236 pPr-CREM...CREM probasin Mammalian Green 8405 p240 pPr-CREM/Ins CREM with insulators probasin Mammalian Green 8406 ...separated by insulator probasin Mammalian Green 11543 pPGK-Cre-bpA Cre PGK Mammalian Rajewsky 11916 pBS185 CMV-Cre...CMV-Cre Cre hCMV Mammalian Sauer 11918 pBS513 EF1alpha-cre Cre EF-1 alpha Mammalian Sauer 11919 pBS448...

  8. Genetic Code Expansion

    Type
    Collection
    ...(Anl) Mammalian ATG David Tirrell 64915 pMAH-POLY tyrosyl-tRNA synthetase E. coli pBof Mammalian TAG Huiwang...AbK Mammalian TAG Simon Elsaesser 140011 pAS_4xMx PylT_FLAG-Mx PylRS PylRS M. alvus CpK Mammalian TAG ...-aminotyrosine Mammalian Huiwang Ai 154762 pAS_FLAG-Mma PylRS PylRS M. mazei Mammalian TAG Simon Elsaesser...archaeon Mammalian TAG Simon Elsaesser 154764 pAS_FLAG-Mx1201 PylRS PylRS M. alvus Mammalian TAG Simon...acetyl-lysine Mammalian Tao Liu 164081 pCMV-MfPylRS (human-opti) PylRS M. flavescens acetyl-lysine Mammalian Tao...mazei Mammalian TAG Simon Elsaesser 174896 pAS_4xMmaPylT(UUA)_EF1_MmaPylRS PylRS M. mazei Mammalian TAA ...mazei Mammalian TGA Simon Elsaesser 174901 pAS_MmaPylT_EF1_NES-MmaPylRS WT PylRS M. mazei Mammalian TAG ...

  9. Arf GTPase Family

    Type
    Collection
    ...8729 1859 Mammalian (pcDNA3) GEF Psd 5662 1024 Mammalian (pEGFP-C1) GEF Psd2 84249 771 Mammalian (pEGFP-...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf3 377 181 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arf5 381 180 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl1 400 181 Bacterial (pET), Mammalian (pEGFP-N3) Gateway...Bacterial (pET), Mammalian (pcDNA3), Gateway GTPase Arl3 403 182 Bacterial (pET), Mammalian (pcDNA3), Gateway...Bacterial (pET), Mammalian (pcDNA3) GAP Arfgap2 84364 521 Bacterial (pET), Mammalian (pcDNA3) GAP Arfgap3...1704 GAP Arap3 64411 1544 Mammalian (pEGFP-C2) GAP Acap1 9744 740 Mammalian (pFLAG-CMV2) GAP Acap2 23527...

  10. Brain Armamentarium

    Type
    Collection
    ...pAAV-AiE0873m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic...AiP14825: pAAV-AiE0441h_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium...3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) Expression of SYFP2 (yellow fluorescent... pAAV-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4361) Expression of jGCaMP8s in striatal indirect... pAAV-AiE0779m_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4360) Expression of jGCaMP8s in striatal direct...pAAV-AiE0452h_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4035) Expression of CoChR-EGFP in striatal indirect...pAAV-AiE0779m_3xC2-minBG-CoChR-EGFP-WPRE3-BGHpA (Alias: CN4033) Expression of CoChR-EGFP in striatal direct...

  11. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Backbones Mammalian CMV, SV40, EF1a, CAG, Ubc Transient expression - Gradia Lab plasmids for mammalian expression...or miniTurbo in the mammalian nucleus pSF3-ultraID - Expresses ultraID in mammalian cells with the Tet-...) pcDNA3.1-mCherry - Expresses mCherry tag in mammalian cells pBI-MCS-EGFP - Tet-inducible...FLAG-HA-pcDNA3.1 - C-terminal Flag or N-terminal FLAG-HA for mammalian expression pNIC-CTHF - C-terminal ...HA Epitope tag pcDNA3.1-HA or c-Flag pcDNA3 - Mammalian expression vector with N- or C-terminal HA tag...Myc Epitope tag pKMyc - N-terminal Myc tag for mammalian expression pGEX-4T-1-3xMyc - Bacterial vector ...pEZYmyc-His - C-terminal Myc-His tag for mammalian expression (Gateway) pYIC - Bicistronic fluorescent...

  12. Fluorescent Protein Guide: FRET

    Type
    Collection
    ...pAquaN1 Cyan Mammalian Expresses mammalian optimized Aquamarine mCerulean N1 Cyan Mammalian Express a gene...subcellular compartments pCEP4CyPet-MAMM Cyan Mammalian Expresses mammalian optimized CyPet pCyPet-His Cyan Bacterial...Yellow Mammalian Expresses mammalian optimized YPet pYPet-His Yellow Bacterial Expresses YPet with C-terminal...Yellow Mammalian Express a gene of interest fused to the C-terminus of SYFP2 Clover Green Mammalian Expresses...N-terminus of monomeric Cerulean mCerulean C1 Cyan Mammalian Express a gene of interest fused to the C-terminus...C-terminus of monomeric Cerulean mTurquoise2 Cyan Mammalian Constructs to target mTurquoise2 to various subcellular...Expresses CyPet with C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest fused to the C-terminus...

  13. Luciferase Plasmid Collection

    Type
    Collection
    ...Luciferase-pcDNA3 Firefly CMV Mammalian expression of firefly luciferase William Kaelin 21375 pHIV-Luciferase... CMV Mammalian expression of Nano-lantern Takeharu Nagai 87121 pcDNA-RLuc8 Renilla CMV Mammalian expression...that has been codon optimized for expression in mammalian systems. Other luciferases in our collection include...has also been codon optimized ( CBR2opt ) for mammalian expression systems. Each type of luciferase has...noted, these plasmids are meant for expression in mammalian systems. ID Plasmid Luciferase Type(s) Description...of a CMV promoter is present for normalization William Kaelin 71248 pXPG Firefly Insertion of 5' promoter... expression of luciferase in the cytoplasm of mammalian cells Peter Cockerill 64784 pGL3-Basic-IRES Firefly...

  14. Caltech Systemic Capsids

    Type
    Collection
    ...AiP13044 - pAAV-AiE0779m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3044) minBG SYFP2 Control AIBS , Ting 191707 ...AiP12237 - pAAV-AiE0452h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2237) minBG SYFP2 Control AIBS , Ting 191720 ...AiP13038 - pAAV-AiE0743m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3038) minBG SYFP2 Control AIBS , Ting 191726 ...AiP12787 - pAAV-AiE0140h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2787) minBG SYFP2 Control AIBS , Ting 191728 ...AiP12408 - pAAV-AiE0600m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2408) minBG SYFP2 Control AIBS , Ting 191737 ...AiP12610 - pAAV-AiE0780m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2610) minBG SYFP2 Control AIBS , Ting 199773 ...AiP13863 - pAAV-AiE0888m_C4-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3863) minBG SYFP2 Control AIBS , Ting 199774 ...

  15. CRISPR Plasmids - Prime Edit

    Type
    Collection
    ...available for expression in mammalian systems, bacteria, plants, and Drosophila. Mammalian ID Plasmid Gene/Insert...GG-acceptor Mammalian hU6 epegRNA BsaI No mRFP1 David Liu 174039 pU6-tmpknot-GG-acceptor Mammalian hU6 epegRNA...Libraries gRNAs Empty gRNA Vectors gRNAs By Species Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish...Cas9 Selection PI 132777 pU6-pegRNA-GG-acceptor Mammalian hU6 pegRNA BsaI No mRFP1 David Liu 140448 QPM-... No Vermilion Norbert Perrimon 164423 pHSG1C3 Mammalian U6 pegRNA + nicking sgRNA BbsI for sgRNAs; BbsI...sgRNA No Tilmann Weber 173220 pPBT-peRNA_GG-Puro Mammalian, piggyBac hU6 pegRNA BsmBI No Puromycin Jacob ...Giehm Mikkelsen 173222 pPBT-PE2-PuroTK-pegRNA_GG Mammalian, piggyBac hU6 pegRNA BsmBI Yes (Cas9 H840A + MMLV...

  16. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...3xFNLDD in mammalian cells 48880 3xFNLDD/pMXs-I2 iChIP plasmid, expresses 3xFNLDD in mammalian cells 48972...3xFNLDD in mammalian cells 49536 3xFNLDD/pMXs-puro iChIP plasmid, expresses 3xFNLDD in mammalian cells 51240...7.1 enChIP plasmid, expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...3xFLAG-dCas9/pMXs-puro Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...3xFLAG-dCas9/pMXs-IG Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...3xFLAG-dCas9/pMXs-I2 Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...3xFLAG-dCas9/pMXs-neo Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic...

  17. Zinc Finger Consortium: Nuclease Expression Vectors

    Type
    Collection
    ... nuclease domains: pST1374 (for expression in mammalian cells or zebrafish) pDW1775 (for expression in... domain. Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish (can be used as a substitute...domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMLM802 (for expression of...domain ( Miller et al., Nat Biotech 2007 ) in mammalian cells or zebrafish). pMG10 (a '“2-in-1” ZFN cassette...domain ( Sollu et al., Nucleic Acids Res ) in mammalian cells or zebrafish). pCLR2068 (for expression ...domain. ( Doyon et al., Nat Methods 2011 ) in mammalian cells or zebrafish as described by Moore et al...

  18. TALEN Plasmids and Kits

    Type
    Collection
    ...Destination constructs were designed for optimal mammalian expression of Voytas Golden Gate-assembled TALEN...destination vectors and a reporter vector for mammalian cell-based validation assay that are designed ...vectors, pcDNA-TAL-NC2 and pCAGGS-TAL-NC2, are mammalian expression/mRNA synthesis vectors containing unique...Torres-Padilla These plasmids were designed for mammalian expression of fluorescent TAL effectors to visualize...plasmids were optimized for TALEN expression in mammalian systems including hard-to-transfect cell lines...sequence to drive efficient TALEN expression in mammalian cells, (iv) an improved, truncated TALE backbone...and pTAL7b for enrichment of double-transfected mammalian cells. In addition, a T7 promoter was included...

  19. Plan Your Experiment

    Type
    Collection
    ...Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or gRNA in a mammalian cell line...Although we will use the example of CRISPR/Cas9 in mammalian cells, many of these principles apply to using...dCas9-KRAB is more effective than dCas9 alone for mammalian cell lines. Activate (CRISPRa) Increase expression...expression systems and variables for using CRISPR in mammalian cells. Some of the variables include: Species ...Expression System Components of System Application Mammalian expression vector Cas enzyme promoter can be constitutive...expression of Cas9 and/or gRNA in a wide variety of mammalian cell lines Useful for difficult to transfect cell...Video Reprint: Protocol for genomic deletions in mammalian cell lines Select Your Target Sequence and Design...

  20. Immunology Research Plasmids and Resources

    Type
    Collection
    ...8 CKLFSF8, CKLFSF8-V2 CNTF ciliary neurotrophic factor HCNTF CNTFR ciliary neurotrophic factor receptor...glucagon-like peptide 2 receptor - GMFB glia maturation factor, beta GMF GMFG glia maturation factor, gamma GMF-...vascular endothelial growth factor A MGC70609, MVCD1, VEGF, VEGF-A, VPF VEGFB vascular endothelial growth...Categories Plasmid Tables Additional Resources Mammalian organisms are exposed to millions of potential...histocompatibility complex, class II, DQ alpha 1 CD, CELIAC1, DQ-A1, FLJ27088, FLJ27328, GSE, HLA-DQA, MGC149527...histocompatibility complex, class II, DQ beta 1 CELIAC1, HLA-DQB, IDDM1 HLA-DRA major histocompatibility...HsT17083, P58, PI-PLC PROCR protein C receptor, endothelial (EPCR) CCCA, CCD41, CD201, EPCR, MGC23024, bA42O4.2...
Showing: 1 - 20 of 96 results