Addgene

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Twenty Years of Addgene Sharing: CRISPR

Type

Blog Post

...popular CRISPR tools is both enduring and rapidly recognized. Where is the CRISPR community? Addgene exists...
Plasmids 101: Degron Tags

Type

Blog Post

...protein of interest, there is also a protease recognition site. In unperturbed conditions, the protease...
Plasmids 101: 5 factors to help you choose the right cloning method

Type

Blog Post

... end construct does not contain the type IIS recognition sites, allowing you to keep the type IIS enzymes...
Addgene's Cancer Collection Pages Connect the Research Community

Type

Blog Post

...safety net is our own immune system, which can recognize antigens on cancerous cells and eliminate them...
Google Forums Round Up: First Impressions of NgAgo

Type

Blog Post

...NgAgo does not require a PAM sequence for target recognition, which gives a researcher unprecedented freedom...
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Type

Blog Post

...For his many efforts in open science, Endy was recognized earlier this year by the White House as a Champion...
Plasmids 101: Secondary Nanobody Toolbox

Type

Blog Post

...subclasses, so that secondary antibodies exclusively recognize only one primary antibody. This process can be...
Transferable Skills Guide: Identifying Your Transferable Skills

Type

Blog Post

...the authors noted that “Many trainees may not recognize the degree of teamwork ability they have developed...
Better Dyeing Through Chemistry & Small Molecule Fluorophores

Type

Blog Post

...solution, but high fluorescence upon binding to its cognate protein. In some cases this property eliminates...
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

Type

Blog Post

...in 2009. However, iChIP requires insertion of recognition sequences of an exogenous DNA-binding protein...
Bioinformatics at Addgene

Type

Blog Post

...the steps into a continuous pipeline. We also recognized the software system that we chose needed to fulfill...
Antibodies 101: Immunoprecipitation

Type

Blog Post

...capture antibody for IP, be sure to choose one that recognizes the target protein in its native conformation...
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Type

Blog Post

...program definitely caused a positive stir and was recognized by meeting organizers in their town hall. Iterative...
Lab to Office Culture Shock

Type

Blog Post

...lentivirus production, I didn’t really start to recognize my transferable (or “soft”) skills until I was...
pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Type

Blog Post

...vectors in human cells [8]. The EBNA-1 protein recognizes OriP and induces amplification of the episomes...
Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

Type

Blog Post

...fashion to the Cre system. The FLP recombinase recognizes FRT sites and initiates site directed gene recombination...
Fluorescent Proteins 101: Aptamer Fluorophores

Type

Blog Post

...immunogenic than proteins. Assays that require recognition of ions or particularly small molecules that ...
Plasmids 101: Gateway Cloning

Type

Blog Post

...proper protein expression elements (ribosome recognition sequences, start codon, stop codons, reading ...
Antibiotic Resistance: An Old Solution but a New Problem

Type

Blog Post

...antibiotics in animal farming is already being recognized as an important step, as you can’t walk into ...
Changing Labor Laws Bring Increased Postdoc Wages

Type

Blog Post

...Harvard) and MIT BCS (Department of Brain and Cognitive Sciences) and the Whitehead Institute (at MIT)...

Showing: 201 - 220 of 258 results

Results per page:

Twenty Years of Addgene Sharing: CRISPR

Plasmids 101: Degron Tags

Plasmids 101: 5 factors to help you choose the right cloning method

Addgene's Cancer Collection Pages Connect the Research Community

Google Forums Round Up: First Impressions of NgAgo

Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

Plasmids 101: Secondary Nanobody Toolbox

Transferable Skills Guide: Identifying Your Transferable Skills

Better Dyeing Through Chemistry & Small Molecule Fluorophores

Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More

Bioinformatics at Addgene

Antibodies 101: Immunoprecipitation

Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

Lab to Office Culture Shock

pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

Fluorescent Proteins 101: Aptamer Fluorophores

Plasmids 101: Gateway Cloning

Antibiotic Resistance: An Old Solution but a New Problem

Changing Labor Laws Bring Increased Postdoc Wages

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