We narrowed to 952 results for: org

...nonprofit or U.S. governmental research organizations, and organizations that require the H-1B employee to ...entity, or nonprofit research organizations or governmental research organizations. Since there is no numerical...Additionally, since most universities and non-profit organizations do not have the April 1 H-1B queue process or... or quota like industry, these organizations can be a good choice for individuals needing sponsorship.... in your field. Membership in a professional organization – this is not referring to one that you can ...program self-driving cars, tissue engineering for organ replacement).  In addition to presenting your expert...These candidates should also join professional organizations and attend the annual conferences – i.e. Society...

...the 4D Nucleome with CRISPR/Cas9 Resources at Addgene.org Read our CRISPR Guide to Epigenetics References...somatic enhancers. Nat Genet 52, 819–827. https://doi.org/10.1038/s41588-020-0639-9.  Feinberg A, Vogelstein...normal counterparts. Nature 301, 89–92. https://doi.org/10.1038/301089a0. Galonska C, Charlton J, Mattei...methyltransferase footprints. Nat Commun 9, 597. https://doi.org/10.1038/s41467-017-02708-5 Hrit J, Goodrich L, ...function in development. eLife 7:e34870. https://doi.org/10.7554/eLife.34870.  Kaas GA, Zhong C, Eason DE...Memory Formation. Neuron 79, 6 P1086-1093. https://doi.org/10.1016/j.neuron.2013.08.032.  Morita S, Noguchi...fusions. Nat Biotechnol 34, 1060–1065. https://doi.org/10.1038/nbt.3658. Sapozhnikov DM, Szyf M (2021)...

...multiplex immunolabeling of brain. eLife 8: . https://doi.org/10.7554/elife.43322 Baker M (2015) Reproducibility...on the antibodies. Nature 521:274–276 . https://doi.org/10.1038/521274a Begley CG, Ellis LM (2012) Raise... cancer research. Nature 483:531–533 . https://doi.org/10.1038/483531a Bradbury A, Plückthun A (2015) ...antibodies used in research. Nature 518:27–29 . https://doi.org/10.1038/518027a Bradbury AM, Plückthun A (2015)...recombinants once. Nature 520:295–295 . https://doi.org/10.1038/520295b Bradbury ARM, Trinklein ND, Thie...functional variable regions. mAbs 10:539–546 . https://doi.org/10.1080/19420862.2018.1445456 Crosnier C, Staudt...monoclonal antibodies. BMC Biology 8:76 . https://doi.org/10.1186/1741-7007-8-76 Greenfield, E.A. (Eds.),...

... Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520 Chavez A, Scheiman J, Vora S... programming. Nat Methods 12:326–328 . https://doi.org/10.1038/nmeth.3312 Chavez A, Tuttle M, Pruitt BW...multiple species. Nat Methods 13:563–567 . https://doi.org/10.1038/nmeth.3871 Konermann S, Brigham MD, Trevino...CRISPR-Cas9 complex. Nature 517:583–588 . https://doi.org/10.1038/nature14136 Perez-Pinera P, Kocak DD, Vockley...transcription factors. Nat Methods 10:973–976 . https://doi.org/10.1038/nmeth.2600 Qi LS, Larson MH, Gilbert LA... Gene Expression. Cell 152:1173–1183 . https://doi.org/10.1016/j.cell.2013.02.022 Sajwan S, Mannervik ...differ in target preference. Sci Rep 9: . https://doi.org/10.1038/s41598-019-54179-x Tanenbaum ME, Gilbert...

...different organizations and individuals, to identify ways of collaborating, and to organize events that...professional development (PD) workshops for teachers; organizing Congressional briefings in Washington DC; convening...Hollywood; and working directly with community organizations, libraries, youth groups, and faith institutions...studies (STS). I also worked with a number of organizations in public engagement efforts, including giving...middle and high school students. One of these organizations was pgEd. After obtaining my PhD and working...often “all hands on deck” to take care of the organizational logistics. Setting yourself on the path to ...audiences. Many universities or cities have organizations (often volunteer-based) that put on public lectures...

..."href":"https://datahub.addgene.org/","href_with_scheme":"https://datahub.addgene.org/","type":"EXTERNAL...Nature Reviews Immunology, 10(5), 301–316. https://doi.org/10.1038/nri2761 Chiu, M. L., Goulet, D. R., Teplyakov...Engineering therapeutics. Antibodies, 8(4), 55. https://doi.org/10.3390/antib8040055 Holliger, P., & Hudson, P....Nature Biotechnology, 23(9), 1126–1136. https://doi.org/10.1038/nbt1142 Kang, B. H., Lax, B. M., & Wittrup.... Methods in Molecular Biology, 29–62. https://doi.org/10.1007/978-1-0716-2285-8_2 McMahon, C., Baier,...Structural & Molecular Biology, 25(3), 289–296. https://doi.org/10.1038/s41594-018-0028-6 Packer, M. S., & Liu,...Nature Reviews Genetics, 16(7), 379–394. https://doi.org/10.1038/nrg3927 Wang, Y., Xue, P., Cao, M., Yu,...

... https://doi.org/10.1016/j.ymthe.2005.02.021 Zuk, P., Sanchez, C. E., Kostick, K., Torgerson, L., Muñoz..., 1–12. https://doi.org/10.3389/fnhum.2020.578687 Additional Resources on addgene.org  Download Addgene's...AAV PHP.B. Gene Therapy, 25(5), 392–400. https://doi.org/10.1038/s41434-018-0028-5 de Backer, M. W. A., ...Hypothalamus. Human Gene Therapy, 21(6), 673–682. https://doi.org/10.1089/hum.2009.169 Gruntman, A. M., Su, L., Su...Human Gene Therapy Methods, 26(2), 71–76. https://doi.org/10.1089/hgtb.2015.040 Initiative, F. B. (2019).... Gene Therapy Methods, 30(6), 214–225. https://doi.org/10.1089/hgtb.2019.105 Rumachik, N. G., Malaker,...Clinical Development, 18(September), 98–118. https://doi.org/10.1016/j.omtm.2020.05.018 Tedersoo, L., Küngas...

...CRISPR effector. Science 353:aaf5573 . https://doi.org/10.1126/science.aaf5573 Broughton JP, Deng X, Yu...detection of SARS-CoV-2. Nature Biotechnology. https://doi.org/10.1038/s41587-020-0513-4 Chen JS, Ma E, Harrington... DNase activity. Science 360:436–439 . https://doi.org/10.1126/science.aar6245 Gootenberg JS, Abudayyeh...CRISPR-Cas13a/C2c2. Science 356:438–442 . https://doi.org/10.1126/science.aam9321 Gootenberg JS, Abudayyeh...Cas12a, and Csm6. Science 360:439–444 . https://doi.org/10.1126/science.aaq0179 Myhrvold C, Freije CA, ...using CRISPR-Cas13. Science 360:444–448 . https://doi.org/10.1126/science.aas8836 Niewoehner O, Jinek M (...CRISPR-associated protein Csm6. RNA 22:318–329 . https://doi.org/10.1261/rna.054098.115 Shmakov S, Abudayyeh OO,...

...path.   Yeast as a model organism Yeast are single cell, eukaryotic organisms formally classified as fungi...know that yeast is a powerful eukaryotic model organism – its genome is easy to manipulate, it’s affordable...cerevisiae (budding). Yeast make for an excellent model organism for a few reasons: Affordable to culture and...studies Peroxisomes are small, membrane-bound organelles essential for sequestering certain enzymes and... methanol metabolism occurs in methylotrophic organisms. K. phaffii is an excellent model for peroxisome...et al., Komagataella phaffi as Emerging Model Organisms in Fundamental Research. Front. Microbiol. 11-...Resources on the Addgene Blog Five Popular Model Organisms Plasmids 101: Yeast Vectors Tips for Screening...

... Vectors. Microorganisms, 12(2), 310. https://doi.org/10.3390/microorganisms1202031038399714. PMID: 38399714...Vectors 101: Systemic Capsids More resource on Addgene.org Addgene's AVV guide AAV Viral Preps References...Expression. Hum Gene Ther, 31(3–4), 151–162. https://doi.org/10.1089/hum.2019.274. PMID: 31914802. Savy, A.,...Hum Gene Ther Methods, 28(5), 277–289. https://doi.org/10.1089/hgtb.2016.133. PMID: 28967288. Shitik, ... modification. Front Med, 10, 1106085. https://doi.org/10.3389/fmed.2023.1106085. PMID: 36817775. Wilmott...Hum Gene Ther Methods, 30(6), 206–213. https://doi.org/10.1089/hgtb.2019.276. PMID: 31752513. Zhou, Q....transgene expression. Sci Rep, 7(1), 5432. https://doi.org/10.1038/s41598-017-04054-4. PMID: 28710345....

...Collection are sorted by organism (see screenshot above). Don’t see your favorite organism listed? Help us serve...us know about other organisms we should include by emailing us at help@addgene.org. If you are looking...plasmids made in your lab, see www.addgene.org/deposit or email deposit@addgene.org for more information or ...to narrow your keyword search to one of these organisms. Help us continue to develop resources for your...like to get the word out about a particular microorganism, microbial technique, or microbial technology...

...with Addgene Senior Scientist, Matt Ferenc, to organize the CRISPR forum's most popular questions and ...Genes Genomes Genetics, 3(4), 657–664. https://doi.org/10.1534/g3.112.005439  Cong, L., Ran, F. A., Cox... systems. Science, 339(6121), 819–823. https://doi.org/10.1126/science.1231143  Elliott, B., Richardson...Molecular and Cellular Biology, 18(1), 93–101. https://doi.org/10.1128/mcb.18.1.93  Ran, F. A., Hsu, P. D., ... specificity. Cell, 154(6), 1380–1389. https://doi.org/10.1016/j.cell.2013.08.021  Ran, F. A., Hsu, ...system. Nature Protocols, 8(11), 2281–2308. https://doi.org/10.1038/nprot.2013.143 Sander, J. D., & Joung... Nature Biotechnology, 32(4), 347–355. https://doi.org/10.1038/nbt.2842  Soldner, F., Laganière, J., ...

... ACS Chemical Neuroscience 6:476–484 . https://doi.org/10.1021/cn500325v Goutaudier, R., Coizet, V., Carcenac...Ligands. Eneuro, 6(5), ENEURO.0171-19.2019. https://doi.org/10.1523/ENEURO.0171-19.2019Jendryka M, Palchaudhuri...chemogenetics in mice. Scientific Reports 9: https://doi.org/10.1038/s41598-019-41088-2 Magnus C, Lee P, Bonaventura...applications. Science 6436: eaav5282. https://science.sciencemag.org/content/364/6436/eaav5282.long Redfern ...mice. Nature Biotechnology 17:165–169 . https://doi.org/10.1038/6165 Roth BL (2016) DREADDs for Neuroscientists...Neuroscientists. Neuron 89:683–694 . https://doi.org/10.1016/j.neuron.2016.01.040 Saloman, J. L., Scheff, N... of Neuroscience, 36(42), 10769–10781. https://doi.org/10.1523/JNEUROSCI.3480-15.2016 Weston M, Kaserer...

...from organization to organization, so you’ll need to be familiar with your specific organization/department...-2 labs. When working with lentivirus, many organizations require or recommend disposable gowns over your...cabinet, even if it is not required by their organization. It’s always okay to add another level of protection..., 10% bleach, or 1% iodine are useful. Some organizations or cities may require a higher percentage of...cut. Following all sharps procedures for your organization will significantly reduce the risk of an accident...also need to familiarize yourself with your organization’s reporting mechanisms before you start an experiment.... Happy transducing! Resources Resources on Addgene.org Addgene viral services Addgene Biosafety Guide...

...CRISPR-Cas systems. A full list of Acr proteins organized by the type of CRISPR-Cas system they block can...anti-CRISPR protein. Nature 546:436–439 . https://doi.org/10.1038/nature22377 Harrington LB, Doxzen KW, Ma... CRISPR-Cas9. Cell 170:1224-1233.e15 . https://doi.org/10.1016/j.cell.2017.07.037 Li C, Psatha N, Gil ...Methods & Clinical Development 9:390–401 . https://doi.org/10.1016/j.omtm.2018.04.008 Marino ND, Pinilla-Redondo...technologies. Nat Methods 17:471–479 . https://doi.org/10.1038/s41592-020-0771-6 Mayo-Muñoz D, He F, Jørgensen...islandicus Rod-Shaped Virus 2. Viruses 10:695 . https://doi.org/10.3390/v10120695 Pawluk A, Amrani N, Zhang Y, ...for CRISPR-Cas9. Cell 167:1829-1838.e9 . https://doi.org/10.1016/j.cell.2016.11.017 Shin J, Jiang F, Liu...

...}{"url":{"content_id":null,"href":"https://www.addgene.org/crispr/empty-grna-vectors/","type":"EXTERNAL...prime editors. Nat Biotechnol 38:824–844 . https://doi.org/10.1038/s41587-020-0561-9 Dempster JM, Pacini C...dependency data sets. Nat Commun 10: . https://doi.org/10.1038/s41467-019-13805-y Doench JG (2017) Am ...genetic screens. Nat Rev Genet 19:67–80 . https://doi.org/10.1038/nrg.2017.97 Hanna RE, Doench JG (2020) ...experiments. Nat Biotechnol 38:813–823 . https://doi.org/10.1038/s41587-020-0490-7 Radzisheuskaya A, Shlyueva...repression. Nucleic Acids Res 44:e141–e141 . https://doi.org/10.1093/nar/gkw583 Rees HA, Liu DR (2018) Base ...living cells. Nat Rev Genet 19:770–788 . https://doi.org/10.1038/s41576-018-0059-1 Veres A, Gosis BS, Ding...

...to deliver a standard AdV vector to the targeted organs or tissues with Cas9 and a gRNA designed to target...Genome Editing. Circ Res 115:488–492 . https://doi.org/10.1161/circresaha.115.304351 Holkers M, Maggio... nucleases. Nat Methods 11:1051–1057 . https://doi.org/10.1038/nmeth.3075 Lukashev AN, Zamyatnin AA Jr...perspectives. Biochemistry Moscow 81:700–708 . https://doi.org/10.1134/s0006297916070063 Maddalo D, Manchado E...CRISPR/Cas9 system. Nature 516:423–427 . https://doi.org/10.1038/nature13902 Maggio I, Holkers M, Liu J,...diverse array of human cells. Sci Rep 4: . https://doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva...Journal of Controlled Release 298:128–141 . https://doi.org/10.1016/j.jconrel.2019.02.009 Wang D, Mou H, Li...

...CRISPR–Cas system. Nature 577:271–274 . https://doi.org/10.1038/s41586-019-1849-0 Klompe SE, Vo PLH, Halpin-Healy... DNA integration. Nature 571:219–225 . https://doi.org/10.1038/s41586-019-1323-z Peters JE, Makarova KS...Proc Natl Acad Sci USA 114:E7358–E7366 . https://doi.org/10.1073/pnas.1709035114 Rubin BE, Diamond S, Cress...Within Microbial Communities. bioRxiv https://www.biorxiv.org/content/10.1101/2020.07.17.209189v2 Strecker...CRISPR-associated transposases. Science 365:48–53 . https://doi.org/10.1126/science.aax9181 Strecker J, Ladha A, Makarova... transposases.” Science 368:eabb2920 . https://doi.org/10.1126/science.abb2920 Vo PLH, Ronda C, Klompe...bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587-020-00745-y Zhang Y, Sun X, Wang...

...blog posts about plasmid cloning Resources on Addgene.org Download the Plasmids 101 eBook  Browse plasmids...low-copy number plasmids. Plasmid 80:54–62 . https://doi.org/10.1016/j.plasmid.2015.03.007 Pubmed PMID: 25889267... Molecular Microbiology 56:1430–1440 . https://doi.org/10.1111/j.1365-2958.2005.04643.x Pubmed PMID: 15916596...plasmids in vivo. PLOS ONE 12:e0172913 . https://doi.org/10.1371/journal.pone.0172913 Pubmed PMID: 28245276...Plasmid Incompatibility. PLoS ONE 7:e29875 . https://doi.org/10.1371/journal.pone.0029875 Pubmed PMID: 22253811...Letters in Applied Microbiology 47:235–240 . https://doi.org/10.1111/j.1472-765x.2008.02426.x Pubmed PMID: 19241516...Opinion in Structural Biology 22:72–79 . https://doi.org/10.1016/j.sbi.2011.11.001 Pubmed PMID: 22153351...

...Why have zebrafish become such a popular model organism? Zebrafish (Figure 1) were initially recognized...reasons that zebrafish have been embraced as a model organism, I’ll outline a few of the available genetic resources...reason for the success of zebrafish as a model organism is its amenability to genetic manipulation. Tools...Europe, and China. In addition, the Zebrafish Model Organism Database ZFIN actively curates information on ...Generating zebrafish transgenics Creating transgenic organisms by introducing foreign DNA into the genome is ...fertilized zebrafish zygote transforms into an organism with a clearly established body plan including...pinhead—contribute to the “sculpting” of tissues and organs during embryo development. Single cell sequencing-based...