Making CRISPR Plasmids Using Fragmid
Type
Blog Post
...contain an ampicillin resistance cassette and an origin of replication, which are not schematized. Sizes...
We narrowed to 303 results for: rigi
-
-
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...check out these two excellent articles: On the Origin of Science Writers How to create a successful science... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post... Förster Resonance Energy Transfer (FRET) was originally described by Theodor Förster in 1948 as a variation... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog Post... higher titer of lentiviral particles than the original lentiCRISPR v1. Special note from the Zhang lab... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...yeast overexpression vector. This plasmid contains origins of replication and selectable markers that allow... -
Viral Vectors 101: Chemogenetics
TypeBlog Post...our chemogenetics poster! This blog post was originally written by Maya Peters Kostman in May 2020 and... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog Post...health status, diet, cage enrichment, stress level, origin (vendor or in-house mouse breeding colony) Environment... -
Mouse Modeling, Part 1: Genetically Engineered Mice
TypeBlog Post.../c, and 129. Conversely, outbred strains were originally derived from random crosses between mice of uncharacterized... -
Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation
TypeBlog Post...dCas9. Within a few rounds of cell division, the original methylated strand is diluted to undetectable amounts... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post... the amount of your protein of interest in the original sample. The competitive ELISA is best used for... -
A Look Back at One Year of Plasmid Sharing for COVID-19 Research
TypeBlog Post...Originally published Apr 30, 2020 and last updated Jul 12, 2021. What started out as a small collection... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog Post...Ward lab created a tool-kit to expand upon these original tools that allows for both spatiotemporal protein... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog Post...consists of 3 components: a deaminase (TadA, originally from E.coli, named TadA7.10 in ABE7.10) a catalytically... -
Plasmids 101: Modular Cloning
TypeBlog Post...functional relevance following a set hierarchy. MoClo originally developed from Golden Gate Cloning, in which ... -
Adapting Toehold Switches to Detect Zika Virus
TypeBlog Post...-specific diagnostics for the Ebola virus. Our original proof-of-concept work was exciting because it ... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...PCR product that comes from amplification of the original plasmid which can over-inflate results. A packing... -
Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015
TypeBlog Post...Szostak. Dr. Szostak’s lab continues its work on the origin of cellular life. His lab has long hypothesized... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog Post...expressed in normal individuals (in a parent-of-origin-specific manner), but the expression or silencing... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog Post...human neuronal cells by human adenoviruses and the origin of HEK 293 cells. The FASEB Journal, 16(8), 869...